LOCUS       sequence-49036-e        1488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FH
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga tggcaagcca aaattccttc cggatagaat atgatacctt tggtgaacta
      121 aaggtgccaa atgataagta ttatggcgcc cagaccgtga gatctacgat gaactttaag
      181 attggaggtg tgacagaacg catgccaacc ccagttatta aagcttttgg catcttgaag
      241 cgagcggccg ctgaagtaaa ccaggattat ggtcttgatc caaagattgc taatgcaata
      301 atgaaggcag cagatgaggt agctgaaggt aaattaaatg atcattttcc tctcgtggta
      361 tggcagactg gatcaggaac tcagacaaat atgaatgtaa atgaagtcat tagcaataga
      421 gcaattgaaa tgttaggagg tgaacttggc agcaagatac ctgtgcatcc caacgatcat
      481 gttaataaaa gccagagctc aaatgatact tttcccacag caatgcacat tgctgctgca
      541 atagaagttc atgaagtact gttaccagga ctacagaagt tacatgatgc tcttgatgca
      601 aaatccaaag agtttgcaca gatcatcaag attggacgta ctcatactca ggatgctgtt
      661 ccacttactc ttgggcagga atttagtggt tatgttcaac aagtaaaata tgcaatgaca
      721 agaataaaag ctgccatgcc aagaatctat gagctcgcag ctggaggcac tgctgttggt
      781 acaggtttaa atactagaat tggctttgca gaaaaggttg ctgcaaaagt ggctgcactt
      841 acaggcttgc cttttgtcac tgctccgaat aaatttgaag ctctggctgc tcatgacgct
      901 ctggttgagc tcagtggagc catgaacact actgcctgca gtctgatgaa gatagcaaat
      961 gatattcgat ttttgggttc tggtcctcgg tcaggtctgg gagaattgat cttgcctgaa
     1021 aatgaaccag gaagcagtat catgccaggc aaggtgaacc ctactcagtg tgaagcaatg
     1081 accatggttg cagcccaagt catggggaac catgttgctg tcactgtcgg aggcagcaat
     1141 ggacattttg agttgaatgt tttcaagcca atgatgatta aaaatgtgtt acactcagcc
     1201 aggctgctgg gggatgcttc agtttccttt acagaaaact gcgtggtggg aatccaggcc
     1261 aatacagaaa ggatcaacaa gctgatgaat gagtctctaa tgttggtgac agctctcaat
     1321 cctcatatag ggtatgacaa ggcagcaaag attgctaaga cagcacacaa aaatggatca
     1381 accttaaagg aaactgctat cgaacttggc tatctcacag cagagcagtt tgacgaatgg
     1441 gtaaaaccta aggacatgct gggtccaaag tgacagtaaa ggtggata
//
