LOCUS       sequence-490369-       13327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor construct for introduction of hNIL factors to CLYBL safe 
            harbor site and iPSC differentiation to motor neuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13327)
  AUTHORS   Fernandopulle MS, Prestil R, Grunseich C, Wang C, Gan L, Ward ME
  TITLE     Transcription Factor-Mediated Differentiation of Human iPSCs into 
            Neurons.
  JOURNAL   Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. 
            Epub 2018 May 18.
  PUBMED    29924488
REFERENCE   2  (bases 1 to 13327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Cell Biol."; date: "2018-06"; volume: "79(1):e51. doi"; pages: " 
            10.1002/cpcb.51. Epub 2018 May 18"
COMMENT     Sequence Label: pUCM-CLYBL-hNIL
FEATURES             Location/Qualifiers
     source          1..13327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1391..1409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1427..1445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1463..1481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1499..1517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1535..1553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1571..1589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1607..1625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1670..1694
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2614..2670
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      3461..3470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3742..3795
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5027..5148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5064..5083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5118..5137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5197..5213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5197..5213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5210..5232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5221..5237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5245..5251,5252..5269,5270..5275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5290..5311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5428..5445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5737..5756
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5755..5810
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5856..5875
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(5966..6712)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6744..6763)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6771..7787
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6825..6847)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        8111..8490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8496..8720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8709..8726
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    8742..8775
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9051..9070)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9320..9338
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      9480..9489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9527..9795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9808..10019)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    10055..10279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(10284..11084)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GPRIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10395..10414)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11005..11024
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(11079..11132)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    11156..11181
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    11182..11215
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(12073..12141,12142..12933)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12291..12310)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13104..13327,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc gaattcgccc ttagagcaga
      421 gccaggaacc cctgtaggga aggggcagga gagccagggg catgagatgg tggacgagga
      481 agggggacag ggaagcctga gcgcctctcc tgggcttgcc aaggactcaa acccagaagc
      541 ccagagcagg gccttaggga agcgggacgg ctctgttgga gagtccagta ttgaattagt
      601 gatagataca cagaaaaaaa gcaaattgaa taggtgaatg gataaacaaa ctgtggaaca
      661 tccagacaat gaaatattca ccaataaaaa gaaatgagct atcaagccac attggctatg
      721 cccttcacat ttaaggagga accttaaatg catattgcca aattttaaaa gctagtctga
      781 aaatgctaca gactgtatga ttccaattat atgacatttt agaaaaggca aaactataga
      841 gatcagtggt tgccagcggc tcatggaggc aggatgaatt ggtggaacac agggatgctt
      901 tcagggtaat gaaaatatct tgtgtgatcc tgtaataatg gatacgtggc ataatgcatt
      961 tgttaaaacc tgtaaactag ccaggtatag tggctttttt gcacctatag tctcagctac
     1021 ttggtaggct gagacaggaa gatcacttga gcccaggact tcaagaccag cctgggtaat
     1081 atagtaagac cccatctcta ttttttaaag aaattaagaa agaaaagaaa cccatcaact
     1141 gtataacaca aagaatcgta atgtaaacta cagtcctgag taatgaaaca tttttaaaga
     1201 aataaataaa ttcaaggcaa ttattaactt caggaaaaac agtaaagtaa acataagtat
     1261 acaaatcaaa catggctcag ttgtgaaaat atttacaagg tcactttaag taacattgaa
     1321 tgttgatata atcccaaaat atatttatgt tggaaggatg aggagatatc tccaccccac
     1381 agtggtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     1441 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     1501 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     1561 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     1621 agagaacgta taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
     1681 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt
     1741 accacttcct accctcgtaa acttaaggtt aattaagccg ccaccatgtt cgtcaaatcc
     1801 gagaccttgg agttgaagga ggaagaggac gtgttagtgc tgctcggatc ggcctccccc
     1861 gccttggcgg ccctgacccc gctgtcatcc agcgccgacg aagaagagga ggaggagccg
     1921 ggcgcgtcag gcggggcgcg tcggcagcgc ggggctgagg ccgggcaggg ggcgcggggc
     1981 ggcgtggctg cgggtgcgga gggctgccgg cccgcacggc tgctgggtct ggtacacgat
     2041 tgcaaacggc gcccttcccg ggcgcgggcc gtctcccgag gcgccaagac ggccgagacg
     2101 gtgcagcgca tcaagaagac ccgtagactg aaggccaaca accgcgagcg aaaccgcatg
     2161 cacaacctca acgcggcact ggacgcgctg cgcgaggtgc tccccacgtt ccccgaggac
     2221 gccaagctca ccaagatcga gaccctgcgc ttcgcccaca actacatctg ggcactcacc
     2281 gagaccctgc gcctggcgga tcactgcggg ggcggcggcg ggggcctgcc gggggcgctc
     2341 ttctccgagg cagtgttgct gagcccggga ggcgccagcg ccgccctgag cagcagcgga
     2401 gacagcccct cgcccgcctc cacgtggagt tgcaccaaca gccccgcgcc gtcctcctcc
     2461 gtgtcctcca attccacctc cccctacagc tgcactttat cgcccgccag cccggccggg
     2521 tcagacatgg actattggca gcccccacct cccgacaagc accgctatgc acctcacctc
     2581 cccatagcca gggattgtat cttgggatcc ggagctacta acttcagcct gctgaagcag
     2641 gctggagacg tggaggagaa ccctggacct atgggagaca tgggagatcc accaaaaaaa
     2701 aaacgtctga tttccctatg tgttggttgc ggcaatcaga ttcacgatca gtatattctg
     2761 agggtttctc cggatttgga atggcatgcg gcatgtttga aatgtgcgga gtgtaatcag
     2821 tatttggacg agagctgtac atgctttgtt agggatggga aaacctactg taaaagagat
     2881 tatatcaggt tgtacgggat caaatgcgcc aagtgcagca tcggcttcag caagaacgac
     2941 ttcgtgatgc gtgcccgctc caaggtgtat cacatcgagt gtttccgctg tgtggcctgc
     3001 agccgccagc tcatccctgg ggacgaattt gcgcttcggg aggacggtct cttctgccga
     3061 gcagaccacg atgtggtgga gagggccagt ctaggcgctg gcgacccgct cagtcccctg
     3121 catccagcgc ggccactgca aatggcagcg gagcccatct ccgccaggca gccggccctg
     3181 cggccccacg tccacaagca gccggagaag accacccgcg tgcggactgt gctgaacgag
     3241 aagcagctgc acaccttgcg gacctgctac gccgcaaacc cgcggccaga tgcgctcatg
     3301 aaggagcaac tggtagagat gacgggcctc agtccccgtg tgatccgggt ctggtttcaa
     3361 aacaagcggt gcaaggacaa gaagcgaagc atcatgatga agcaactcca gcagcagcag
     3421 cccaatgaca aaactaatat ccaggggatg acaggaactc ccatggtggc tgccagtcca
     3481 gagagacacg acggtggctt acaggctaac ccagtggaag tacaaagtta ccagccacct
     3541 tggaaagtac tgagcgactt cgccttgcag agtgacatag atcagcctgc ttttcagcaa
     3601 ctggtcaatt tttcagaagg aggaccgggc tctaattcca ctggcagtga agtagcatca
     3661 atgtcctctc aacttccaga tacacctaac agcatggtag ccagtcctat tgaggcattg
     3721 atgcacagca gaggctctgg cgagggcaga ggcagcctgc tgacatgtgg cgacgtggaa
     3781 gagaaccctg gccccggtac catggaagcc agaggcgaac tgggccctgc cagagaatct
     3841 gccggcggag atctgctgct ggccctgctg gctagaaggg ccgacctgag aagagaaatc
     3901 cccctgtgcg ccggctgcga ccagcacatc ctggaccggt ttatcctgaa ggccctggac
     3961 agacactggc acagcaagtg cctgaagtgc agcgactgcc acacccccct ggccgagaga
     4021 tgctttagca gaggcgagag cgtgtactgc aaggacgact tcttcaagag attcggcacc
     4081 aagtgcgccg cctgccagct gggaattcct cctacacagg tcgtgcggag agcccaggac
     4141 ttcgtgtacc atctgcactg cttcgcctgc gtcgtgtgca agagacagct ggccaccggc
     4201 gacgagttct acctgatgga agatagccgg ctcgtgtgta aagccgacta cgagacagcc
     4261 aagcagagag aggccgaggc caccgccaaa agacccagaa ccaccatcac cgccaagcag
     4321 ctggaaaccc tgaagtccgc ctacaacacc agccccaagc ccgccagaca tgtgcgggaa
     4381 cagctgagca gcgagacagg cctggacatg agagtggtgc aagtgtggtt ccagaacaga
     4441 cgggccaaag agaagcggct gaagaaggac gccggcaggc agagatgggg ccagtacttc
     4501 cggaacatga agcggtccag aggcggcagc aagagcgaca aggacagcgt gcaggaaggc
     4561 caggacagcg acgccgaggt gtccttccca gatgagccta gcctggccga aatgggccca
     4621 gccaatggcc tgtatggcag cctgggagag ccaacccagg ctctgggcag accttctggc
     4681 gccctgggca acttctctct ggaacacggc ggactggccg gacccgagca gtatagagaa
     4741 ctgaggcccg gcagccctta cggcgtgcca ccttctccag ctgcccctca gtctctgcca
     4801 ggacctcagc ctctgctgtc cagcctggtg taccccgata caagcctggg cctggtgcca
     4861 tctggcgctc caggcggacc tcctccaatg agagtgctgg ccggcaatgg ccctagcagc
     4921 gatctgagca caggcagctc tggcggctac cccgattttc ctgccagccc tgcctcctgg
     4981 ctggacgaag tggatcacgc ccagttctga atcgatcgcc caccccaact tgtttattgc
     5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5161 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5221 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5281 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5461 gctgcggcga gcggtatcag ctcactcaaa ggacggccgc acatgtaagg aaaattttag
     5521 ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa
     5581 ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact gatgacctct
     5641 ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca aactctaaac
     5701 caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc cgagtgagag
     5761 acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt
     5821 cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctca
     5881 gtggtatttg tgagccaggg cattggccac accagtcacc accttctgat aggcagcctg
     5941 cacctgagga gtgaattcac gcgttttagc ctggcagcat atcgagatcg aagtcatcga
     6001 gagcgtcagc agggagcata tccagatcaa aatcatccag ggcatcggcg ggcagcatgt
     6061 ccaggtcgaa gtcgtccagg gcgtcggtgg gtccgccgct ctcgcacttc agctgcttct
     6121 cgaggccgca gatgatcagt tccaggccga acaggaaggc gggctcggcg ccctgtctgt
     6181 cgaacagctc gatggcctgc ttcagcaggg ggggcatgct gtcggtggtg ggtgtctctc
     6241 tttcctcttt ggcgacctgg tgttcctgtt cttccagcac gcagcccagg gtgaagtggc
     6301 ccacggcgct cagggcgtac agggcgtttt ccaggctgaa gccctgctgg cacaggaagg
     6361 ccagctggtt ttccagtgtc tcgtactgct tctcggtggg tctggtgccc aggtgcactt
     6421 tggcgccgtc ccggtggctc agcagggcgc atctgtagct cttggcgttg ttccgcagga
     6481 agtcctgcca gctctcgcct tccagagggc agctgtgggt gtggtgccgg tccagcatct
     6541 cgatgggcag ggcgtccagc agggcccgct tgttcttcac gtgccagtac agggtgggct
     6601 gttccacgcc cagtttctgg gccagcttcc gggtggtcag gccctcgatg cccacgccgt
     6661 tcagcagttc cagggcgctg ttgatcactt tgctcttgtc cagccggctc atgtcgacat
     6721 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa
     6781 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
     6841 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga
     6901 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt
     6961 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc
     7021 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac
     7081 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg
     7141 gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc
     7201 acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg
     7261 agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt
     7321 tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg
     7381 cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc
     7441 ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg
     7501 ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc
     7561 gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac
     7621 agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg
     7681 tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag
     7741 gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg
     7801 cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc
     7861 gcgccgctct gattggctgc cgccgcacct ctccgcctcg cccccccccc cccccccccc
     7921 cccccccccc ccccccgcgc cccccccccc cccccccccc cccccccccc cccccccccg
     7981 gccccgcccc cccccccccc cccgccccca tcgctgcaca aaataattaa aaaataaata
     8041 aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag aacgtggggc
     8101 tcacctcgac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     8161 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     8221 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     8281 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     8341 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     8401 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     8461 actattaata actagtcaat aatcaatgtc gagacccata gagcccaccg catccccagc
     8521 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     8581 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     8641 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     8701 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt tataacttcg tataatgtat
     8761 gctatacgaa gttatggtac cttactcgtc catgccgccg gtggagtggc ggccctcggc
     8821 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     8881 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     8941 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     9001 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     9061 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     9121 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     9181 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     9241 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     9301 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     9361 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     9421 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     9481 catggtggcg accggtagcg ctagcgtagg cgccggtcac agcttggatc tgtaacggcg
     9541 cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc aaagcgtgga
     9601 gagccggctg agtctaggta ggctccaagg gagcgccgga caaaggcccg gtctcgacct
     9661 gagctttaaa cttacctaga cggcggacgc agttcaggag gcaccacagg cgggaggcgg
     9721 cagaacgcga ctcaaccggc gtggatggcg gcctcaggta gggcggcggg cgcgtgaagg
     9781 agagatgcga gcccctcgaa gcttcagctg tgttctggcg gcaaacccgt tgcgaaaaag
     9841 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     9901 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     9961 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
    10021 ctgacgggca ccggagcgat cgcagatcat gcatccatag agcccaccgc atccccagca
    10081 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
    10141 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
    10201 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
    10261 tggcaactag aaggcacagg tttttagaag aactcgtcaa gaaggcgata gaaggcgatg
    10321 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
    10381 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
    10441 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
    10501 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
    10561 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
    10621 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
    10681 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
    10741 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
    10801 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
    10861 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
    10921 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
    10981 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
    11041 gccggagaac ctgcgtgcaa tccatcttgt tcaatcctag ggccgggatt ctcctccacg
    11101 tcaccgcatg ttagaagact tcctctgccc tctccgctgc cagatctctc gaggccctgt
    11161 gggaggaaga gaagaggtca gataacttcg tataatgtat gctatacgaa gttatgcggc
    11221 cgctgggaga agggtaagag agctcaatcc tcatcttaca tagaaggaag tcagtagata
    11281 atatcttaca tttaaaagtc aagaaataac agtctgacca tactatctag aaatatggaa
    11341 gttaacagta aggaagttaa aacagttgaa agtagtttcg tctagaaaga aagacttggg
    11401 gaagaacaaa gaaggctaat tttcattatg agctttattg taacagaaaa gagaccacta
    11461 actcagctag gttttcccaa ttcctcaaca ctttggggca cagtgtttag tcagagctgt
    11521 gatcactctc tgagcacatg gattgtatct taattttcat ctcattgaaa atatgtacca
    11581 tttgtccaag tggaaactgg gctgaagcat gactgtgacc accaccaaag aaatggctag
    11641 actcgctaag gtctgctggg gtgacccgtg attgtgttgt gcttgatctt gttgagcatg
    11701 gctcttttaa gtcaccatac ctgcccctgc caatgcacca aacaccaact taaagggact
    11761 ttcagctttt gtaagtcagt ctcagcctta cttctgaacc atttctttga cttctttcat
    11821 gactctgatc ctgtcccagt tttaagcctc ccagccacca gtcatgctgt tttgctctgc
    11881 tatttacatt cttctctacc tactttggac ttgatggtat gcctactata ttagttattt
    11941 attgctgtgt aacaaaccac tctgaaacat agtggcttac aaccacaacc attttatttt
    12001 attatgaaac tgtgggtcag aatgtatatt ggcccattgg catgattctc ctgctattga
    12061 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    12121 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    12181 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    12241 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    12301 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    12361 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    12421 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    12481 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    12541 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    12601 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    12661 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    12721 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    12781 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12841 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12901 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    13021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    13081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    13141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    13201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    13261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    13321 ctaatcc
//
