LOCUS       sequence-49042-e        1811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1811)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HMGCS2
FEATURES             Location/Qualifiers
     source          1..1811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..357
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             364..384
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt atgagcgata aaattattca cctgactgac
       61 gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga tttctgggca
      121 gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc tgacgaatat
      181 cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
      241 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc
      301 aaagtgggcg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggccggt
      361 accgagaact tgtacttcca atccatgcca aaggacgtgg gcatcctggc cctggaggtc
      421 tacttcccag cccaatatgt ggaccaaact gacctggaga agtataacaa tgtggaagca
      481 ggaaagtata cagtgggctt gggccagacc cgtatgggct tctgctcagt ccaagaggac
      541 atcaactccc tgtgcctgac ggtggtgcaa cggctgatgg agcgcataca gctcccatgg
      601 gactctgtgg gcaggctgga agtaggcact gagaccatca ttgacaagtc caaagctgtc
      661 aaaacagtgc tcatggaact cttccaggat tcaggcaata ctgatattga gggcatagat
      721 accaccaatg cctgctacgg tggtactgcc tccctcttca atgctgccaa ctggatggag
      781 tccagttcct gggatggtcg ttatgccatg gtggtctgtg gagacattgc cgtctatccc
      841 agtggtaatg ctcgtcccac aggtggggcc ggagctgtgg ctatgctgat tgggcccaag
      901 gcccctctgg ccctggagcg agggctgagg ggaacccata tggagaatgt gtatgacttc
      961 tacaaaccaa atttggcctc ggagtaccca atagtggatg ggaagctttc catccagtgc
     1021 tacttgcggg ccttggatcg atgttacaca tcataccgta aaaaaatcca gaatcagtgg
     1081 aagcaagctg gcagcgatcg acccttcacc cttgacgatt tacagtacat gatctttcat
     1141 acaccctttt gcaagatggt ccagaagtct ctggctcgcc tgatgttcaa tgacttcctg
     1201 tcagccagca gtgacacaca aaccagctta tataaggggc tggaggcttt cggggggcta
     1261 aagctggaag acacctacac caacaaggac ctggataaag cacttctaaa ggcctctcag
     1321 gacatgttcg acaagaaaac caaggcttcc ctttacctct ccactcacaa tgggaacatg
     1381 tacacctcat ccctgtacgg gtgcctggcc tcgcttctgt cccaccactc tgcccaagaa
     1441 ctggctggct ccaggattgg tgccttctct tatggctctg gtttagcagc aagtttcttt
     1501 tcatttcgag tatcccagga tgctgctcca ggctctcccc tggacaagtt ggtgtccagc
     1561 acatcagacc tgccaaaacg cctagcctcc cgaaagtgtg tgtctcctga ggagttcaca
     1621 gaaataatga accaaagaga gcaattctac cataaggtga atttctcccc acctggtgac
     1681 acaaacagcc ttttcccagg tacttggtac ctggagcgag tggacgagca gcatcgccga
     1741 aagtatgccc ggcgtcccgt ctaacagtaa aggtggatac ggatccgaat tcgagctccg
     1801 tcgacaagct t
//
