LOCUS       sequence-49046-e        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CRYL1
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ccggctgcgt ggtgatcgtt ggcagtggag tcattgggcg aagctgggcc
      121 atgctgtttg ccagtggagg cttccaggtg aaactctatg acattgagca acagcagata
      181 aggaacgccc tggaaaacat cagaaaggag atgaagttgc tggagcaggc aggttctctg
      241 aaaggctccc tgagtgtgga agagcagctg tcactcatca gtggttgtcc caatatccaa
      301 gaagcagtag agggtgccat gcacattcag gaatgtgttc cagaagatct agaactgaag
      361 aagaagattt ttgctcagtt agattccatc attgatgatc gagtgatctt aagcagttcc
      421 acttcttgtc tcatgccttc caagttgttt gctggcttgg tccatgtgaa gcaatgcatc
      481 gtggctcatc ctgtgaatcc gccatactac atcccgctgg ttgagctggt cccccacccg
      541 gagacggccc ctacgacagt ggacagaacc cacgccctga tgaagaagat tggacagtgc
      601 cccatgcgag tccagaagga ggtggccggc ttcgttctga accgcctgca atatgcaatc
      661 atcagcgagg cctggcggct agtggaggaa ggaatcgtgt ctcctagtga cctggacctt
      721 gtcatgtcag aagggttggg catgcggtat gcattcattg gacccctgga aaccatgcat
      781 ctcaatgcag aaggtatgtt aagctactgc gacagataca gcgaaggcat aaaacatgtc
      841 ctacagactt ttggacccat tccagagttt tccagggcca ctgctgagaa ggttaaccag
      901 gacatgtgca tgaaggtccc tgatgacccg gagcacttag ctgccaggag gcagtggagg
      961 gacgagtgcc tcatgagact cgccaagttg aagagtcaag tgtgacagta aaggtggata
//
