LOCUS       sequence-490462-       14681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CaMV 35S driving the expression of dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14681)
  AUTHORS   Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, 
            Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, 
            Secco D, Small I, Kidd BN, Lister R
  TITLE     CRISPRi-based circuits to control gene expression in plants.
  JOURNAL   Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 
            10.1038/s41587-024-02236-w. Epub 2024 May 20.
  PUBMED    38769424
REFERENCE   2  (bases 1 to 14681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-024-02236-w"; journalName: "Nat Biotechnol"; date: 
            "2025-03"; volume: "43"; issue: "3"; pages: "416-430"
COMMENT     Sequence Label: LLP1018
FEATURES             Location/Qualifiers
     source          1..14681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    62..86
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1417..2046
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2475..3548
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3614..3808
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4044..4066)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4152..4292
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4207..4226
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4307..4324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4478..5066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4558..4577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5312..6103)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6624..6648
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6741..6993)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7013..7564)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7606..7785)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8126..8326
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        8377..8721
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8734..8754
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9560..9608
                     /note="synthetic polyadenylation signal"
     misc_feature    9622..9713
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     9662..9681
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        9720..10064
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             10080..10100
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(10113..11549,11739..14402)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          11550..11738
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             14415..14435
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcttagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
       61 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
      121 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
      181 agggttcccc tcgggatcaa agtactttaa agtactttaa agtactttaa agtactttga
      241 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
      301 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
      361 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
      421 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
      481 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
      541 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
      601 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
      661 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
      721 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
      781 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
      841 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
      901 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
      961 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     1021 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     1081 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     1141 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     1201 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     1261 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     1321 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     1381 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     1441 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     1501 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     1561 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     1621 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     1681 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     1741 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     1801 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     1861 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     1921 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     1981 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     2041 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     2101 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     2161 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     2221 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     2281 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     2341 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     2401 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
     2461 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     2521 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     2581 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     2641 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     2701 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     2761 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     2821 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     2881 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     2941 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     3001 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     3061 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     3121 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     3181 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     3241 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     3301 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     3361 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     3421 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     3481 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     3541 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     3601 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     3661 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     3721 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     3781 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     3841 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     3901 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     3961 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     4021 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4081 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4141 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4201 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4261 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     4321 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata tctcccaatt
     5161 tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc tgatagtttg
     5221 gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg
     5281 gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg gtgatctcgc
     5341 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     5401 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     5461 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     5521 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     5581 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     5641 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     5701 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     5761 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     5821 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     5881 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     5941 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     6001 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     6061 ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt aactttgttt
     6121 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
     6181 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt
     6241 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     6301 tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt
     6361 atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc atcgttacaa
     6421 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
     6481 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
     6541 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
     6601 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
     6661 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattgaat
     6721 tatcagcttg catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc
     6781 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     6841 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     6901 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     6961 taagaaactt tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt
     7021 cggtgacggg caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac
     7081 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     7141 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     7201 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     7261 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     7321 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     7381 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     7441 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     7501 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     7561 tcatggtaga tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     7621 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     7681 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     7741 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     7801 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     7861 tcatgatcga taattcgagg gtacaagctt tcgtacgcgt aatcggatct aacgatgagc
     7921 tcaaaacctg agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa
     7981 tatatcaccc gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg
     8041 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaatactgc
     8101 agactgacta actaggatga gctaagctag ctatatcatc aatttatgta ttacacataa
     8161 tatcgcactc agtctttcat ctacggcaat gtaccagctg atataatcag ttattgaaat
     8221 atttctgaat ttaaacttgc atcaataaat ttatgttttt gcttggacta taatacctga
     8281 cttgttattt tatcaataaa tatttaaact atatttcttt caagatggga attaacatct
     8341 acaaattgcc ttttcttatc gaccatgtac catatgtgag acttttcaac aaagggtaat
     8401 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     8461 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     8521 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     8581 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     8641 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     8701 ttcatttcat ttggagagga ccctaggatg gctccaaaaa agaaaagaaa ggttgctagc
     8761 gaccattact tagatattag attgagacca gacccagagt tccctccagc acagttgatg
     8821 tcagtgttat tcggaaaatt gcaccaggct ttggttgcac aaggtggaga tagaattggt
     8881 gtgagttttc ctgatttgga cgagtctagg tcaagattgg gagaaaggtt aagaatccat
     8941 gcttcagcag atgaccttag ggctttgtta gcaagacctt ggttagaggg tcttagagat
     9001 catttgcagt tcggagaacc agctgttgtg ccacacccta caccatatag acaagtttct
     9061 agagtgcagg caaaatcaaa tcctgagaga cttagaagga gattgatgag gagacacgac
     9121 ttatctgaag aggaagctag gaagagaata cctgataccg ttgctagagc acttgacttg
     9181 ccatttgtga ctttgagaag tcaatccact ggtcagcatt ttaggttatt cattagacac
     9241 ggaccacttc aagttactgc tgaggaggga ggattcacct gttatggatt gagtaaggga
     9301 ggattcgtgc cttggttctg aggtacccgg ccatgctaga gtccgcaaaa atcaccagtc
     9361 tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag
     9421 ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     9481 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     9541 aatccagtga cctactagaa ataaaatatc tttattttca ttacatctgt gtgttggttt
     9601 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     9661 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctgaattct
     9721 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     9781 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     9841 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     9901 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     9961 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
    10021 gacccttcct ctatataagg aagttcattt catttggaga ggacggcact agtgatatgc
    10081 caaagaagaa gcggaaggtc gggggtggtt ctgacaagaa gtactctatc ggacttgcca
    10141 tcggaaccaa ctctgttgga tgggctgtta tcaccgatga gtacaaggtt ccatctaaga
    10201 agttcaaggt tcttggaaac accgatagac actctatcaa gaagaacctt atcggtgctc
    10261 ttcttttcga ttctggagag accgctgagg ctaccagatt gaagagaacc gctagaagaa
    10321 gatacaccag aagaaagaac agaatctgct accttcagga aatcttctct aacgagatgg
    10381 ctaaggttga tgattctttc ttccacagac ttgaggagtc tttccttgtt gaggaggata
    10441 agaagcacga gagacaccca atcttcggaa acatcgttga tgaggttgct taccacgaga
    10501 agtacccaac catctaccac cttagaaaga agttggttga ttctaccgat aaggctgatc
    10561 ttagacttat ctaccttgct cttgctcaca tgatcaagtt cagaggacac ttccttatcg
    10621 agggagacct taacccagat aactctgatg ttgataagtt gttcatccag cttgttcaga
    10681 cctacaacca gcttttcgag gagaacccaa tcaacgcttc tggagttgat gctaaggcta
    10741 tcctttctgc tagactttct aagtctcgta gacttgagaa ccttatcgct cagcttccag
    10801 gagagaagaa gaacggactt ttcggaaacc ttatcgctct ttctcttgga cttaccccaa
    10861 acttcaagtc taacttcgat cttgctgagg atgctaagtt gcagctttct aaggatacct
    10921 acgatgatga tcttgataac cttcttgctc agatcggaga tcagtacgct gatcttttcc
    10981 ttgctgctaa gaacctttct gatgctatcc ttctttctga catccttaga gttaacaccg
    11041 agatcaccaa ggctccactt tctgcttcta tgatcaagag atacgatgag caccaccagg
    11101 atcttaccct tttgaaggct cttgttagac agcagcttcc agagaagtac aaggaaatct
    11161 tcttcgatca gtctaagaac ggatacgctg gatacatcga tggaggagct tctcaggagg
    11221 agttctacaa gttcatcaag ccaatccttg agaagatgga tggaaccgag gagcttcttg
    11281 ttaagttgaa cagagaggat cttcttagaa agcagagaac cttcgataac ggatctatcc
    11341 cacaccagat ccaccttgga gagcttcacg ctatccttcg tagacaggag gatttctacc
    11401 cattcttgaa ggataacaga gagaagatcg agaagatcct taccttcaga atcccatact
    11461 acgttggacc acttgctaga ggaaactctc gtttcgcttg gatgaccaga aagtctgagg
    11521 agaccatcac cccttggaac ttcgaggagg taagtttctg cttctacctt tgatatatat
    11581 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa
    11641 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata
    11701 acttttctaa tatatgacca aaatttgttg atgtgcaggt tgttgataag ggagcttctg
    11761 ctcagtcttt catcgagaga atgaccaact tcgataagaa ccttccaaac gagaaggttc
    11821 ttccaaagca ctctcttctt tacgagtact tcaccgttta caacgagctt accaaggtta
    11881 agtacgttac cgagggaatg agaaagccag ctttcctttc tggagagcag aagaaggcta
    11941 tcgttgatct tcttttcaag accaacagaa aggttaccgt taagcagttg aaggaggatt
    12001 acttcaagaa gatcgagtgc ttcgattctg ttgaaatctc tggagttgag gatagattca
    12061 acgcttctct tggaacctac cacgatcttt tgaagatcat caaggataag gatttccttg
    12121 ataacgagga gaacgaggac atccttgagg acatcgttct tacccttacc cttttcgagg
    12181 atagagagat gatcgaggag agactcaaga cctacgctca ccttttcgat gataaggtta
    12241 tgaagcagtt gaagagaaga agatacaccg gatggggtag actttctcgt aagttgatca
    12301 acggaatcag agataagcag tctggaaaga ccatccttga tttcttgaag tctgatggat
    12361 tcgctaacag aaacttcatg cagcttatcc acgatgattc tcttaccttc aaggaggaca
    12421 tccagaaggc tcaggtttct ggacagggag attctcttca cgagcacatc gctaaccttg
    12481 ctggatctcc agctatcaag aagggaatcc ttcagaccgt taaggttgtt gatgagcttg
    12541 ttaaggttat gggtagacac aagccagaga acatcgttat cgagatggct agagagaacc
    12601 agaccaccca gaagggacag aagaactctc gtgagagaat gaagagaatc gaggagggaa
    12661 tcaaggagct tggatctcaa atcttgaagg agcacccagt tgagaacacc cagcttcaga
    12721 acgagaagtt gtacctttac taccttcaga acggaagaga tatgtacgtt gatcaggagc
    12781 ttgacatcaa cagactttct gattacgatg ttgatgctat cgttccacag tctttcttga
    12841 aggatgattc tatcgataac aaggttctta cccgttctga taagaacaga ggaaagtctg
    12901 ataacgttcc atctgaggag gttgttaaga agatgaagaa ctactggaga cagcttctta
    12961 acgctaagtt gatcacccag agaaagttcg ataaccttac caaggctgag agaggaggac
    13021 tttctgagct tgataaggct ggattcatca agagacagct tgttgagacc agacagatca
    13081 ccaagcacgt tgctcagatc cttgattctc gtatgaacac caagtacgat gagaacgata
    13141 agttgatcag agaggttaag gttatcacct tgaagtctaa gttggtttct gatttcagaa
    13201 aggatttcca gttctacaag gttagagaga tcaacaacta ccaccacgct cacgatgctt
    13261 accttaacgc tgttgttgga accgctctta tcaagaagta cccaaagttg gagtctgagt
    13321 tcgtttacgg agattacaag gtttacgatg ttagaaagat gatcgctaag tctgagcagg
    13381 agatcggaaa ggctaccgct aagtacttct tctactctaa catcatgaac ttcttcaaga
    13441 ccgagatcac ccttgctaac ggagagatca gaaagagacc acttatcgag accaacggag
    13501 agaccggaga gatcgtttgg gataagggaa gagatttcgc taccgttaga aaggttcttt
    13561 ctatgccaca ggttaacatc gttaagaaaa ccgaggttca gaccggagga ttctctaagg
    13621 agtctatcct tccaaagaga aactctgata agttgatcgc tagaaagaag gattgggacc
    13681 caaagaagta cggaggattc gattctccaa ccgttgctta ctctgttctt gttgttgcta
    13741 aggttgagaa gggaaagtct aagaagttga agtctgttaa ggagcttctt ggaatcacca
    13801 tcatggagcg ttcttctttc gagaagaacc caatcgattt ccttgaggct aagggataca
    13861 aggaggttaa gaaggatctt atcatcaagt tgccaaagta ctctcttttc gagcttgaga
    13921 acggaagaaa gagaatgctt gcttctgctg gagagcttca gaagggaaac gagcttgctc
    13981 ttccatctaa gtacgttaac ttcctttacc ttgcttctca ctacgagaag ttgaagggat
    14041 ctccagagga taacgagcag aagcagcttt tcgttgagca gcacaagcac taccttgatg
    14101 agatcatcga gcaaatctct gagttctcta agagagttat ccttgctgat gctaaccttg
    14161 ataaggttct ttctgcttac aacaagcaca gagataagcc aatcagagag caggctgaga
    14221 acatcatcca ccttttcacc cttaccaacc ttggtgctcc agctgctttc aagtacttcg
    14281 ataccaccat cgatagaaaa agatacacct ctaccaagga ggttcttgat gctaccctta
    14341 tccaccagtc tatcaccgga ctttacgaga ccagaatcga tctttctcag cttggaggag
    14401 atggtggggg cagtcctaaa aagaaaagga aggtgtaagt cgaccggcca tgctagagtc
    14461 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
    14521 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
    14581 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
    14641 ctaattccta aaaccaaaat ccagtgacct agtcactcga g
//
