LOCUS       sequence-490464-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CaMV 35S driving the expression of dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, 
            Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, 
            Secco D, Small I, Kidd BN, Lister R
  TITLE     CRISPRi-based circuits to control gene expression in plants.
  JOURNAL   Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 
            10.1038/s41587-024-02236-w. Epub 2024 May 20.
  PUBMED    38769424
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-024-02236-w"; journalName: "Nat Biotechnol"; date: 
            "2025-03"; volume: "43"; issue: "3"; pages: "416-430"
COMMENT     Sequence Label: LLP1015
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      680..932
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      962..1162
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             1528..1548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2354..2402
                     /note="synthetic polyadenylation signal"
     misc_feature    2416..2507
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2456..2475
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2514..2858
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2802..2822
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2874..2894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2907..4343,4533..7196)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          4344..4532
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             7209..7229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7489..7507)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7528..7544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7528..7544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7541..7563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7552..7568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7576..7582,7583..7600,7601..7606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7621..7642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7759..7776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7930..8518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8010..8029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8689..9480,9481..9549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9312..9331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9550..9654)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtacaag
      661 cttatctaac gatgagctcg atcgttcaaa catttggcaa taaagtttct taagattgaa
      721 tcctgttgcc ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt
      781 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtcca
      841 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
      901 atcgcgcgcg gtgtcatcta tgttactaga tcctgcagac tgactaacta ggatgagcta
      961 agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac
     1021 ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca
     1081 ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt
     1141 taaactatat ttctttcaag atgggaatta acatctacaa attgcctttt cttatcgacc
     1201 atgtaccata tgccaacact cgaatcccca cccgttggac caaacccggc tcattaagcg
     1261 tcggttcaga tttatttcct ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg
     1321 aaacagttaa agcccaaaat tgtaatttac cgagaattgt aaatttacct gaaaacccta
     1381 cgctatagtt tcgactataa ataccaaact taggacctca cttcagaatc ccctcgtcgc
     1441 tgcgtctctc tcccgcaacc ttcgattttc gtttattcgc atccatcgga gagagaaaac
     1501 aatcaataag cgacccctag gatggctcca aaaaagaaaa gaaaggttgc tagcgaccat
     1561 tacttagata ttagattgag accagaccca gagttccctc cagcacagtt gatgtcagtg
     1621 ttattcggaa aattgcacca ggctttggtt gcacaaggtg gagatagaat tggtgtgagt
     1681 tttcctgatt tggacgagtc taggtcaaga ttgggagaaa ggttaagaat ccatgcttca
     1741 gcagatgacc ttagggcttt gttagcaaga ccttggttag agggtcttag agatcatttg
     1801 cagttcggag aaccagctgt tgtgccacac cctacaccat atagacaagt ttctagagtg
     1861 caggcaaaat caaatcctga gagacttaga aggagattga tgaggagaca cgacttatct
     1921 gaagaggaag ctaggaagag aatacctgat accgttgcta gagcacttga cttgccattt
     1981 gtgactttga gaagtcaatc cactggtcag cattttaggt tattcattag acacggacca
     2041 cttcaagtta ctgctgagga gggaggattc acctgttatg gattgagtaa gggaggattc
     2101 gtgccttggt tctgaggtac ccggccatgc tagagtccgc aaaaatcacc agtctctctc
     2161 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     2221 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     2281 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     2341 gtgacctact agaaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     2401 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     2461 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctgaa ttctgagact
     2521 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
     2581 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
     2641 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     2701 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     2761 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     2821 tcctctatat aaggaagttc atttcatttg gagaggacgg cactagtgat atgccaaaga
     2881 agaagcggaa ggtcgggggt ggttctgaca agaagtactc tatcggactt gccatcggaa
     2941 ccaactctgt tggatgggct gttatcaccg atgagtacaa ggttccatct aagaagttca
     3001 aggttcttgg aaacaccgat agacactcta tcaagaagaa ccttatcggt gctcttcttt
     3061 tcgattctgg agagaccgct gaggctacca gattgaagag aaccgctaga agaagataca
     3121 ccagaagaaa gaacagaatc tgctaccttc aggaaatctt ctctaacgag atggctaagg
     3181 ttgatgattc tttcttccac agacttgagg agtctttcct tgttgaggag gataagaagc
     3241 acgagagaca cccaatcttc ggaaacatcg ttgatgaggt tgcttaccac gagaagtacc
     3301 caaccatcta ccaccttaga aagaagttgg ttgattctac cgataaggct gatcttagac
     3361 ttatctacct tgctcttgct cacatgatca agttcagagg acacttcctt atcgagggag
     3421 accttaaccc agataactct gatgttgata agttgttcat ccagcttgtt cagacctaca
     3481 accagctttt cgaggagaac ccaatcaacg cttctggagt tgatgctaag gctatccttt
     3541 ctgctagact ttctaagtct cgtagacttg agaaccttat cgctcagctt ccaggagaga
     3601 agaagaacgg acttttcgga aaccttatcg ctctttctct tggacttacc ccaaacttca
     3661 agtctaactt cgatcttgct gaggatgcta agttgcagct ttctaaggat acctacgatg
     3721 atgatcttga taaccttctt gctcagatcg gagatcagta cgctgatctt ttccttgctg
     3781 ctaagaacct ttctgatgct atccttcttt ctgacatcct tagagttaac accgagatca
     3841 ccaaggctcc actttctgct tctatgatca agagatacga tgagcaccac caggatctta
     3901 cccttttgaa ggctcttgtt agacagcagc ttccagagaa gtacaaggaa atcttcttcg
     3961 atcagtctaa gaacggatac gctggataca tcgatggagg agcttctcag gaggagttct
     4021 acaagttcat caagccaatc cttgagaaga tggatggaac cgaggagctt cttgttaagt
     4081 tgaacagaga ggatcttctt agaaagcaga gaaccttcga taacggatct atcccacacc
     4141 agatccacct tggagagctt cacgctatcc ttcgtagaca ggaggatttc tacccattct
     4201 tgaaggataa cagagagaag atcgagaaga tccttacctt cagaatccca tactacgttg
     4261 gaccacttgc tagaggaaac tctcgtttcg cttggatgac cagaaagtct gaggagacca
     4321 tcaccccttg gaacttcgag gaggtaagtt tctgcttcta cctttgatat atatataata
     4381 attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt
     4441 agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt
     4501 ctaatatatg accaaaattt gttgatgtgc aggttgttga taagggagct tctgctcagt
     4561 ctttcatcga gagaatgacc aacttcgata agaaccttcc aaacgagaag gttcttccaa
     4621 agcactctct tctttacgag tacttcaccg tttacaacga gcttaccaag gttaagtacg
     4681 ttaccgaggg aatgagaaag ccagctttcc tttctggaga gcagaagaag gctatcgttg
     4741 atcttctttt caagaccaac agaaaggtta ccgttaagca gttgaaggag gattacttca
     4801 agaagatcga gtgcttcgat tctgttgaaa tctctggagt tgaggataga ttcaacgctt
     4861 ctcttggaac ctaccacgat cttttgaaga tcatcaagga taaggatttc cttgataacg
     4921 aggagaacga ggacatcctt gaggacatcg ttcttaccct tacccttttc gaggatagag
     4981 agatgatcga ggagagactc aagacctacg ctcacctttt cgatgataag gttatgaagc
     5041 agttgaagag aagaagatac accggatggg gtagactttc tcgtaagttg atcaacggaa
     5101 tcagagataa gcagtctgga aagaccatcc ttgatttctt gaagtctgat ggattcgcta
     5161 acagaaactt catgcagctt atccacgatg attctcttac cttcaaggag gacatccaga
     5221 aggctcaggt ttctggacag ggagattctc ttcacgagca catcgctaac cttgctggat
     5281 ctccagctat caagaaggga atccttcaga ccgttaaggt tgttgatgag cttgttaagg
     5341 ttatgggtag acacaagcca gagaacatcg ttatcgagat ggctagagag aaccagacca
     5401 cccagaaggg acagaagaac tctcgtgaga gaatgaagag aatcgaggag ggaatcaagg
     5461 agcttggatc tcaaatcttg aaggagcacc cagttgagaa cacccagctt cagaacgaga
     5521 agttgtacct ttactacctt cagaacggaa gagatatgta cgttgatcag gagcttgaca
     5581 tcaacagact ttctgattac gatgttgatg ctatcgttcc acagtctttc ttgaaggatg
     5641 attctatcga taacaaggtt cttacccgtt ctgataagaa cagaggaaag tctgataacg
     5701 ttccatctga ggaggttgtt aagaagatga agaactactg gagacagctt cttaacgcta
     5761 agttgatcac ccagagaaag ttcgataacc ttaccaaggc tgagagagga ggactttctg
     5821 agcttgataa ggctggattc atcaagagac agcttgttga gaccagacag atcaccaagc
     5881 acgttgctca gatccttgat tctcgtatga acaccaagta cgatgagaac gataagttga
     5941 tcagagaggt taaggttatc accttgaagt ctaagttggt ttctgatttc agaaaggatt
     6001 tccagttcta caaggttaga gagatcaaca actaccacca cgctcacgat gcttacctta
     6061 acgctgttgt tggaaccgct cttatcaaga agtacccaaa gttggagtct gagttcgttt
     6121 acggagatta caaggtttac gatgttagaa agatgatcgc taagtctgag caggagatcg
     6181 gaaaggctac cgctaagtac ttcttctact ctaacatcat gaacttcttc aagaccgaga
     6241 tcacccttgc taacggagag atcagaaaga gaccacttat cgagaccaac ggagagaccg
     6301 gagagatcgt ttgggataag ggaagagatt tcgctaccgt tagaaaggtt ctttctatgc
     6361 cacaggttaa catcgttaag aaaaccgagg ttcagaccgg aggattctct aaggagtcta
     6421 tccttccaaa gagaaactct gataagttga tcgctagaaa gaaggattgg gacccaaaga
     6481 agtacggagg attcgattct ccaaccgttg cttactctgt tcttgttgtt gctaaggttg
     6541 agaagggaaa gtctaagaag ttgaagtctg ttaaggagct tcttggaatc accatcatgg
     6601 agcgttcttc tttcgagaag aacccaatcg atttccttga ggctaaggga tacaaggagg
     6661 ttaagaagga tcttatcatc aagttgccaa agtactctct tttcgagctt gagaacggaa
     6721 gaaagagaat gcttgcttct gctggagagc ttcagaaggg aaacgagctt gctcttccat
     6781 ctaagtacgt taacttcctt taccttgctt ctcactacga gaagttgaag ggatctccag
     6841 aggataacga gcagaagcag cttttcgttg agcagcacaa gcactacctt gatgagatca
     6901 tcgagcaaat ctctgagttc tctaagagag ttatccttgc tgatgctaac cttgataagg
     6961 ttctttctgc ttacaacaag cacagagata agccaatcag agagcaggct gagaacatca
     7021 tccacctttt cacccttacc aaccttggtg ctccagctgc tttcaagtac ttcgatacca
     7081 ccatcgatag aaaaagatac acctctacca aggaggttct tgatgctacc cttatccacc
     7141 agtctatcac cggactttac gagaccagaa tcgatctttc tcagcttgga ggagatggtg
     7201 ggggcagtcc taaaaagaaa aggaaggtgt aagtcgaccg gccatgctag agtccgcaaa
     7261 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga
     7321 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     7381 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     7441 cctaaaacca aaatccagtg acctagtcac tcgagccagc ttttgttccc tttagtgagg
     7501 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8341 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9661 tccccgaaaa gtgc
//
