LOCUS       sequence-490573-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for multi-site piggybac transposon insertion of luciferase 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  TITLE     Bartel lab plasmids 2025
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DBJS-p8.38
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(245..349)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(375..830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(512..533)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     724..743
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     957..979
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     971..988
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     972..988
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1118..1180
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1626..1911
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     CDS             2643..3119
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             3129..3644
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        7198..7409
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             7422..7690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      7718..7727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7724..8122
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8155..8276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8192..8211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8246..8265
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9039..9073
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9564..9580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9564..9580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9577..9599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9588..9604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9612..9618,9619..9636,9637..9642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9657..9678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9795..9812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9966..10554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10046..10065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10725..11341,1..175,176..244))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
       61 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      361 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      421 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      481 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      541 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      601 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      661 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      721 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      781 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
      841 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      901 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      961 gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg tttttgaacc
     1021 cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa
     1081 gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata atcatattgt
     1141 gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg gtctgtatat
     1201 cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca cttacatact
     1261 aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa aaacaaaaac
     1321 tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc cccaaagccc
     1381 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     1441 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     1501 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     1561 cagacacctg gggggatacg gggaaaaggc ctccacggcc actagtcttt cgtcttcaag
     1621 aattccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1681 ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1741 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1801 attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg
     1861 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     1921 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     1981 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcc
     2041 rggsgggggg gggggggggg gggggggggg gggggsgrgg sggrgrggkg cggcggcagc
     2101 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     2161 ctataaaaag cgaagcgcgc ggcgggcggg rgtcgctgcg cgctgccttc gccccgtgcc
     2221 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     2281 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg
     2341 gtttaaggga tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga
     2401 aatcactttt tttcaggttg gaccggtgct actgcgagga gccggcgcag ggtggcccgg
     2461 gaggggtgag cagggtgccg ctggctgctg gggtctgcag gtcaccgagt ccccaggaga
     2521 ggggactcct aagaagccac ctgcctgtgt ttacccggca gcgagcgcgc aggcccccgc
     2581 gaactcctgg cagcgctcag gaaaggccgt tgcgcctcgc gaaggaaaca gagccgttga
     2641 ccatgatcag tctgattgcg gcgttagcgg tagattacgt tatcggcatg gaaaacgcca
     2701 tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg caacacctta aataaacccg
     2761 tgattatggg ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga cgcaaaaata
     2821 ttatcctcag cagtcaaccg agtacggacg atcgcgtaac gtgggtgaag tcggtggatg
     2881 aagccatcgc ggcgtgtggt gacgtaccag aaatcatggt gattggcggc ggtcgcgtta
     2941 ttgaacagtt cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac gcagaagtgg
     3001 aaggcgacac ccatttcccg gattacgagc cggatgactg ggaatcggta ttcagcgaat
     3061 tccacgatgc tgatgcgcag aactctcaca gctattgctt tgagattctg gagcggcgag
     3121 gctccggaat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct
     3181 acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg
     3241 tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca
     3301 tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa
     3361 tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca
     3421 cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag
     3481 gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca
     3541 aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca
     3601 acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaagggcca gggaagaact
     3661 ggctcggaaa gaggacccca catgttgtag cttcaagttt aaacggatcc aacctacaaa
     3721 cgggtggagg atcaccccac ccgacacttc acaatcaagg ggtacaatac acaagggtgg
     3781 aggaacaccc caccctccag acacattaca cagaaatcca atcaaacaga agcaccatca
     3841 gggcttctgc taccaaattt atctcaaaaa actacaacaa ggaatcacca tcagggattc
     3901 cctgtgcaat atacgtcaaa cgagggccac gacgggagga cgatcacgcc tcccgaatat
     3961 cggcatgtct ggctttcgaa ttcagtgcgt ggagcatcag cccacgcagc caatcagagt
     4021 cgaatacaag tcgactttcg cgaagagcat cagccttcgc gccattctta cacaaaccac
     4081 actctcccct acaggaacag catcagcgtt cctgcccagt acccaactca agaaaattta
     4141 tgtccccatg cagcatcagc gcatgggccc caagaataca tccccaacaa aatcacatcc
     4201 gagcaccaac agggctcgga gtgttgtttc ttgtccaact ggacaaaccc tccatggacc
     4261 atcaggccat ggactctcac caacaagaca aaaactactc ttctcgaagc agcatcagcg
     4321 cttcgaaaca ctcgagcata cattgtgcct atttcttggg tggacgatca cgccacccat
     4381 gctctcacga atttcaaaac acggacaagg acgagcacca ccagggctcg tcgttccacg
     4441 tccaatacga ttacttacct ttcgggatca cgatcacgga tcccgcagct acatcacttc
     4501 cactcaggac attcaagcat gcacgatcac ggcatgctcc acaagtctca accacagaaa
     4561 ctaccaaatg ggttcagcac cagcgaaccc actcctacct caaacctctt cccacaaaac
     4621 tggcaagcag gatcaccgct tgcccattcc aacataccaa atcaaaaaca attactggta
     4681 cagcatcagc gtaccagccc acatctctca ctactatcaa aaaccaaacc gttcagcaac
     4741 agcgaacggt acacacggaa aaatcaactg gtttacaaat acgaaagacg atcacgcttt
     4801 cgtccagcgc aaactattac gaaaaacatc cgacgggaag agcaacagcc ttcccgcggc
     4861 ggaaaacctc acaaaaacac gacaaacgga tgcacgaaca cggcatccgc cgacaaccca
     4921 caaacttaca accaggcaaa cggtgcagga tcaccgcacc gtacatcaaa cacctcagat
     4981 ctcatttcgc gcgggatcca gaccacctcc cctgcgagct aagctggaca gccaatgacg
     5041 ggtaagagag tgacattttt cactaaccta agacaggagg gccgtcagag ctactgccta
     5101 atccaaagac gggtaaaagt gataaaaatg tatcactcca acctaagaca ggcgcagctt
     5161 ccgagggatt tgaatcgatt tcgcgcaaag ggaaaatata taaagattat ttcatgattg
     5221 ggtagtaaaa ctattcagct agtcagctaa agtcatttgt agtttgcccc acctgcccta
     5281 aataagaaac cccaaatgta gtctcttttc tttctgtgtt tcacattcat agcaactgca
     5341 gctaacaggc tgattttctg gcctttggag aagtgattca aaatagtgta gattttctgc
     5401 atagatccca tttttgtaca gaattgaatg ggatggaata ggtaagcaaa agtagaagcc
     5461 catttgagtt ttacatttga ttccacaatt tggtttcagg taggcttggt aatagactat
     5521 ataaaccaga tttgcctatt ttgattttca tatggctttt ttttctgtaa gttttcagag
     5581 gattttttaa atcacagaat catactaaat gatatttagc ctatcaaaac ttccaaaagc
     5641 ccacaccacc agttcctgac tcaaatttga agggttttta gacaggaggg taggattaag
     5701 taggtgagtt taattaaagc ttaaccctag gtaagagtaa atgagaaata ttacggcaat
     5761 aatggaactg cttcactgtt tcttggtgac ttcctcactc taatgtttta aagaggcaac
     5821 aaaagcttgt ggtgccattt cagtaaccac ggtgttgttt tagatgcctt tataagctca
     5881 gtttcccctg ttcttaagtg ttgaatactg tctttaaact agaaaaatgc aaaatattga
     5941 actgatattt ttgtgtgtag ttgattactc ttccattgag tgaatgatga atacctgtga
     6001 ggataggaaa ttagttctga gatctagtcc ctctctgatt cacttagtaa tctatcctct
     6061 tttcagtatt acatgtgctt aatctcagat gaaccatttc accatggcag tgttatctca
     6121 tctctgggct tttctgggaa ttgaagtatc tctccttaac cccaattgtc aagggtagta
     6181 gctgtatact accactttga attattgaaa cgggtcaatt tacgaagtct gcattggcta
     6241 tggagatatg gtttatagta cagcctagag aatgaaactc accgtccaga taaccatgca
     6301 tgcacccaga ttttttccac cttggatacc tgtcactagg gaataataaa ggcctgattt
     6361 tttgtcttat tccaactaag tagatcatta tctctttcct tttttatgtt aatgagagaa
     6421 tttagcctcc actcaacaat gttcaattca gcaaggcttt catatccttg ctgtgggtcg
     6481 tggataagga gcttattcag gtttcctgcc ctagctatta gctccacttc acatgctgga
     6541 gaccggcgta gggacagatg tattcatcct ggtgttactg aaaaacaggt gtgatcctgt
     6601 tactgatact ataagtgacc taaaatgtca ctgttcaaat tagccagtgt tctaacaaac
     6661 taaactcttc aaatgcttgg aaagatacta caaagccaat ctttatagaa ttgggccaag
     6721 ataaatctat gttgttttgc atggctattg ttaagctcca aaggttcact gtgtttctgc
     6781 cgctgtcctg gagttgtcac cactgactgg gcaaggcttc ttgggcatgg atgtagaact
     6841 gttgtccttt tcccactaac agttatcttt gactctcttg cctgttatgc ttacaaaatg
     6901 gtgatggctt atggaaggct gttaaattaa tattcctgtt aaaggaaatt aaagtttgtc
     6961 tatttttgac aataaaacat tatatatttt taattctctt gtcctctttt tttttctttc
     7021 acctacttgg tgcagtggtc ataggatcag ggctgcaatt acaagggctg taagtaggga
     7081 tggctcatta taaaccttat atcagaattc cttatgggcc tgatagaagt gggttgtgcc
     7141 ttaaatcgga tccagctgaa tctaagtgga tctgcgatcg ctccggtgcc cgtcagtggg
     7201 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     7261 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     7321 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     7381 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     7441 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     7501 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     7561 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     7621 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     7681 gttacagatc taagctgtga ccggcgccta ctctagagcc gccatggcca agcctttgtc
     7741 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     7801 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     7861 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     7921 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     7981 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     8041 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     8101 tggttatgtg tgggagggct gaaatcaacc tctggattac aaaatttgtg ggtaaacttg
     8161 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8221 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8281 gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg gtctcccttc
     8341 cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac tgggttctta
     8401 tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga acgaagtccc
     8461 taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt ttttagggcc
     8521 cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     8581 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     8641 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     8701 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     8761 ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     8821 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     8881 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     8941 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
     9001 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     9061 tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt ttttccggct
     9121 cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc gtgccttttc
     9181 ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct gcattgacgt
     9241 tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc acgcctttaa
     9301 cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt tccggacaca
     9361 gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg acagccggaa
     9421 ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt acgtcagcga
     9481 ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg agttacccgg
     9541 cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10381 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11341 a
//
