LOCUS       sequence-490594-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTNNA1 M-domain deletion mutant with enhanced actin-binding domain 
            mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Wang Y, Yemelyanov A, Go CD, Kim SK, Quinn JM, Flozak AS, Le PM, 
            Liang S, Gingras AC, Ikura M, Ishiyama N, Gottardi CJ
  TITLE     alpha-Catenin force-sensitive binding and sequestration of LZTS2 
            leads to cytokinesis failure.
  JOURNAL   J Cell Biol. 2025 Mar 3;224(3):e202308124. doi: 
            10.1083/jcb.202308124. Epub 2025 Jan 9.
  PUBMED    39786338
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-03-3"; volume: "224(3):e202308124. doi"; pages: 
            " 10.1083/jcb.202308124. Epub 2025 Jan 9"
COMMENT     Sequence Label: pLV-mEGFP-hCTNNA1_DM1-3_H0-ABD+
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2010..2313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2314..2517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2468..2488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2538..2562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2570..2579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2576..2578,2579..2581,2582..3292)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2621..2642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2882..2901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3229..3250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4961..4985)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5017..5228
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5241..5509
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5537..6136
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5537..5556)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6033..6053
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6137..6157)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6170..6758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6223..6243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6641..6652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6846..7079
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7151..7272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7188..7207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7242..7261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7312..7447
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7467..7486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7468..7486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7496..7513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7496..7512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7505..7527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7654..8109
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7741..7760)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7951..7972
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8135..8239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8240..8308,8309..9100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8458..8477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9271..9859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9760..9779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10013..10030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10147..10168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10183..10188,10189..10206,10207..10213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10221..10237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10226..10248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10245..10261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10245..10261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10280..10300
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10282..10300
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgt agttattaat agtaatcaat tacggggtca
     1981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2161 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2221 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2461 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     2521 ttagtgaacc gtcagatcca agtttgtaca aaaaagcagg ctctcgagtg ccaccatggt
     2581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3181 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg
     3301 cagcggcagc tctagaatga ctgctgtcca tgcaggcaac ataaacttca agtgggatcc
     3361 taaaagtcta gagatcagga ctctggcagt tgagagactg ttggagcctc ttgttacaca
     3421 ggttacaacc cttgtaaaca ccaatagtaa agggccctct aataagaaga gaggtcgttc
     3481 taagaaggcc catgttttgg ctgcatctgt tgaacaagca actgagaatt tcttggagaa
     3541 gggggataaa attgcgaagg agagccagtt tctcaaggag gagcttgtgg ctgctgtaga
     3601 agatgttcga aaacaaggtg atttgatgaa ggctgctgca ggagagttcg cagatgatcc
     3661 ctgctcttct gtgaagcgag gcaacatggt tcgggcagct cgagctttgc tctctgctgt
     3721 tacccggttg ctgattttgg ctgacatggc agatgtctac aaattacttg ttcagctgaa
     3781 agttgtggaa gatggtatct tgaagttgag gaatgctggc aatgaacaag acttaggaat
     3841 ccagtataaa gccctaaaac ctgaagtgga taagctgaac attatggcag ccaaaagaca
     3901 acaggaattg aaagatgttg gccatcgtga tcagatggct gcagctagag gaatcctgca
     3961 gaagaacgtt ccgatcctct atactgcatc ccaggcatgc ctacagcacc ctgatgtcgc
     4021 agcctataag gccaacaggg acctgatata caagcagctg cagcaggcgg tcacaggcat
     4081 ttccaatgca gcccaggcca ctgcctcaga cgatgcctca cagcaccaga ggacccctga
     4141 ggagttggat gactctgact ttgagacaga agattttgat gtcagaagca ggacgagcgt
     4201 ccagacagaa gacgatcagc tgatagctgg ccagagtgcc gggtcgggct cggctcagct
     4261 tccccaggag caaaaagcga agattgcgga acaggtggcc agcttccagg aagaaaagag
     4321 caagctggat gctgaagtgt ccaaatggga cgacagtggc aatgacatca ttgtgctggc
     4381 caagcagatg tgcatgatta tgatggagat gacagacttt acccgaggta aaggaccact
     4441 caaaaataca tcggatgtca tcagtgctgc caagaaaatt gctgaggcag gatccaggat
     4501 ggacaagctt ggccgcacca ttgcagacca ttgccccgac tcggcttgca agcaggacct
     4561 gctggcctac ctgcaacgca tcgccctcta ctgccaccag ctgaacatct gcagcaaggt
     4621 caaggccgag gtgcagaatc tcggcgggga gcttgttgtc tctggggtgg acagcgccat
     4681 gtccctgatc caggcagcca agaacttgat gaatgctgtg gtgcagacag tgaaggcatc
     4741 ctacgtcgcc tctaccaaat accaaaagtc acagggtatg gcttccctca accttcctgc
     4801 tgtgtcatgg aagatgaagg caccagagaa aaagccattg gtgaagagag agaaacagga
     4861 tgagacacag accaagatta aacgggcatc tcagaagaag cacgtgaacc cggtgcaggc
     4921 cctcagcgag ttcaaagcta tggacagcat ctaatctaga acccagcttt cttgtacaaa
     4981 gtggaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     5041 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     5101 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     5161 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     5221 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     5281 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     5341 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     5401 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     5461 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     5521 cggcgcctac gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5581 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5641 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5701 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     5761 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5821 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5881 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5941 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6001 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6061 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6121 caagcccggt gcctgacaac tttattatac atagttgatc aattccgata atcaacctct
     6181 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6241 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6301 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6361 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6421 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6481 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6541 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6601 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6661 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6721 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcc cgcggttcgc
     6781 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6841 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7081 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7141 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7261 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7381 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7441 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     7501 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7561 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7621 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7801 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     8101 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8461 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8761 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9661 gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg gacaggtatc cggtaagcgg
     9721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10141 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10201 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10261 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
    10321 agctt
//
