LOCUS       sequence-490602-       11733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible AIRE-FLAG expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11733)
  TITLE     Sun Hur lab AIRE plasmids
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pInducer20 AIRE-FLAG
FEATURES             Location/Qualifiers
     source          1..11733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..32
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(251..355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     423..441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             560..793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    868..1456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             974..985
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1383..1403
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1625..2419)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1625..2419)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1736..1755)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2346..2365
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    2435..3008
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2595..2614)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2824..2841
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(3060..3068,3069..3104,3105..3107,3108..3143,
                     3144..3146,3147..3182,3183..3188,3189..3806))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(3865..5076)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4009..4028)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(5162..5188)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(5202..5225)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6857..6866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6868..6877
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6987..7011)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7008..7046)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(7079..7097,7098..7120,7121..7139,7140..7162,7163..7181,
                     7182..7204,7205..7223,7224..7246,7247..7265,7266..7288,
                     7289..7307,7308..7330,7331..7349)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    7404..7521
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7814..7858)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8043..8276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8773..8898
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8945..9578
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9770..9789)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9839..9855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9839..9855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9852..9874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9863..9879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9887..9893,9894..9911,9912..9917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9932..9953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10193..10210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10364..10952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10444..10463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11123..11733,1..181,182..250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
       61 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      361 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      421 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatg atctagccct ttccttaatt
      481 aacccgggcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
      541 agatgacatg aactactact gctagagatt ttccacactg actaaaaggg tctgagggat
      601 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
      661 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
      721 tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga
      781 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
      841 taagtcattg gtcttaaagg atctcaggcg gggaggcggc ccaaagggag atccgactcg
      901 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
      961 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     1021 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
     1081 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     1141 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     1201 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     1261 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     1321 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     1381 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     1441 aatccagagg ttgattagga tctatcgcga taagcttgat atcgaattgg gagggactaa
     1501 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1561 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tctttacttg
     1621 tacatcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     1681 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     1741 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     1801 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     1861 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
     1921 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     1981 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     2041 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     2101 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     2161 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     2221 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     2281 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     2341 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     2401 atccatcttg ttcaatcatg gtggaccggt aagcttatca tcgtgttttt caaaggaaaa
     2461 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
     2521 agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
     2581 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
     2641 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
     2701 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
     2761 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
     2821 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
     2881 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
     2941 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg gggggggagg
     3001 gagagggggg gggggagagg ggcggaattc ctctagtgcg ggcgcggatc cttacttagt
     3061 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     3121 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     3181 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     3241 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtctcaga
     3301 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     3361 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     3421 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     3481 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     3541 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     3601 gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     3661 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     3721 ttccttgtcg tcaggccttc gataccgaca ccattgagta attccagagc tccgtttatg
     3781 actttgctct tgtccagtct agacatggtg aattcgactg caggaccggt acggggtgga
     3841 gatccgagct cggtaccaag cttcgtctaa caaaaaagcc aaaaacggcc agaatttagc
     3901 ggacaattta ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa
     3961 aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg
     4021 gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac
     4081 ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc
     4141 cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca
     4201 ccctgcatta taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc
     4261 aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac
     4321 aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc
     4381 ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt
     4441 gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct
     4501 tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag
     4561 ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc
     4621 ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca
     4681 agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa
     4741 ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag
     4801 atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa
     4861 agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact
     4921 ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag
     4981 gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg aggagggggg
     5041 cgcccgcggg aggcgccaaa acccggcgcg gaggccagat cttgggtggg ttactccaga
     5101 ctgccttggg aaaagcgcct cccctacccg gtagaatttc tagtttaatt aatcattact
     5161 aagcgtagtc tgggacgtcg tatgggtaag ggcgcgcctc acttgtcgtc atcatccttg
     5221 taatcggagg ggaagggggc cgccggacgg gccatgctct ggatggccca ctgcaggatg
     5281 ccatcgaagg tgtgctcgct cagaagggac tccaggtcat ccctgtgcag agcgggctcg
     5341 tgactggcag tgtcatcctt ggcaggccca ggggccaggc gggcggggct gggggccagc
     5401 accccctcca caggggctgg ggtcacgtct cctgagcagg atctgcagcg caggcccgtc
     5461 ccgggccggg aggtgccggc tgggaagtgg cagcgccagt ggaaggcagc ggcgcagtga
     5521 gtacaccgca gcacgtccgt accatctccg cacaccccgc aacgcgcacc aggagccagg
     5581 ttctgctgac cctcaggacc cacacacagt agggggtgca gggccgagga gtccagccct
     5641 ggcagcgggg ctgcagaagg cggagccggc aggtgcttgt agacaagagt cgtgtccatg
     5701 ccggctaggg gttccccagg tggacctctt acctcctctc ccgccgacct aagccccggg
     5761 gggagcgggg tctccacggg tggctcctgg ggccggggct cctctgcccg gggctgcacc
     5821 tcctggactg ttgcctgcag gcagctggag cacctccagg tcccactggg gatctcccgg
     5881 agcggagggg acaggcaggc caggtggaag gcccgagggc agccgtcaca gcagatgagc
     5941 tccccgccgt cccgacacac ggcacactcg tcctcattct tctggtggag ctgggggtca
     6001 ctggggaggg ccagaggggc gggaacgctg ccctgctggc ccagcctagc ctcacctcca
     6061 ccgggggcag cgccctgggc tcccttggct cgaaccagag gcttcgggcc actgctgctg
     6121 cgggccttgt tcttcccact gccggagtct tcgaacttgc tgggagtgta gaactcccca
     6181 ccaacctgga tgcacttctt ggagccgcct gactcaaaca cctgctggat gaggatcccc
     6241 tccacggccc ctcgggctcc cgggacgtcc ccggaggaca tggccacagc tctctggact
     6301 gaagctgaca tggtctgaat cccgttcccg agtggaaggc gctgctgctc tgcgctgctc
     6361 tccggcttct tggggggctt ggccttcagt tgagagcctg ggctggcggt gccccttgga
     6421 gtcagggctg ctggcgcggc agctcgagcc tcttctgagg ccttcctctt ggtggggagt
     6481 ctgggtggcg gtaccaaagc cttggggacg gccgggggct tcctcccctt ccggggctgg
     6541 ctgaggtcca catctttggg gaagctgtcc aggatgggct gcagccggcc atagcgctcc
     6601 aggttgtagt ccttgaacag caccctccag aagtccagga tggctgtgga gtcctgggtc
     6661 agcagccagg acaggagggc gtggaaggcc tgggggcagc cctccttttc cttcagatga
     6721 agcgtctcct gaaacttgtc ctcggggacc acgtcgtggt cagccagcgc gtgcagcagt
     6781 gggaaggcgc tgtccacggc caccgcgatc tccgtgcggt gcagcctcag aagccggcgt
     6841 agcgccgcgt ccgtcgccat ggtggcggcc gccatggctg atatctgcag aattccacca
     6901 cactggacta gttcggggcc gcggaggctg gatcggtccc ggtgtcttct atggaggtca
     6961 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc tctgcttata
     7021 taggcctccc accgtacacg cctacctcga cccgggtacc gagctcgact ttcacttttc
     7081 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
     7141 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
     7201 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
     7261 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
     7321 ctttcacttt tctctatcac tgatagggag tggtaaagga tcctagtcca aactggatct
     7381 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     7441 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     7501 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtgaatt cggcgatacc
     7561 gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     7621 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     7681 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     7741 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     7801 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     7861 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     7921 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     7981 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     8041 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     8101 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     8161 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     8221 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     8281 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     8341 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     8401 ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt
     8461 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     8521 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     8581 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     8641 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     8701 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     8761 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     8821 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     8881 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     8941 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     9001 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     9061 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     9121 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     9181 agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct tgtagcaagc
     9241 tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt gatgaaatgc
     9301 taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca tcccatgcag
     9361 gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta ccttatctgg
     9421 ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga cccctggccc
     9481 tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc acagatcaag
     9541 gatatcttgt cttctttggg agtgaattag cccttccaac tactaagttt gtagtacata
     9601 tttaacaaat acaatttctt taaaatgaaa ataattcaga ggaatcacag gtttagagta
     9661 aatgaaacca caggtaattg gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat
     9721 tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg atgagtttgg
     9781 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattc gtaatcatgt
     9841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccacggggag ctttttgcaa
    10021 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
    10081 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttgctg cattaatgaa
    10141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    11641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    11701 agtgtatgcg gcgaccgagt tgctcttgcc cgg
//
