LOCUS       sequence-49062-e        1715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1715)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACAD11
FEATURES             Location/Qualifiers
     source          1..1715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..357
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             364..384
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt atgagcgata aaattattca cctgactgac
       61 gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga tttctgggca
      121 gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc tgacgaatat
      181 cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
      241 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc
      301 aaagtgggcg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggccggt
      361 accgagaact tgtacttcca atccatgtcc aaacgaactt tcagtactgt actaccacag
      421 attgatacta ctggacagtt gtttgtacag actcggaaag gtcaggaagt tcttattaag
      481 gtgaagcatt tcatgaaaca acacattctt ccagctgaaa aggaggtaac tgagttctat
      541 gttcaaaatg aaaattcagt ggacaagtgg ggaaaacctt tagtgattga taaactcaag
      601 gaaatggcca aagtcgaggg tctctggaac ttgtttttgc cagctgtcag cggactcagc
      661 cacgtggact atgccttgat tgctgaagaa acaggaaaat gcttttttgc tccagatgtc
      721 tttaactgcc aagcaccaga cacagggaat atggaggttc tgcacctgta tggaagtgag
      781 gaacagaaga aacagtggct tgagcctctt cttcaaggga acattacctc ttgcttctgt
      841 atgacagaac ctgatgtagc ttcaagtgat gccacgaata ttgaatgcag catccaacga
      901 gatgaagata gctatgtaat taacggcaaa aaatggtgga gcagtggagc tgggaatccc
      961 aagtgcaaaa ttgcaattgt tttgggaaga actcaaaata cttctctctc cagacacaaa
     1021 cagcacagca tgattcttgt tcccatgaac acacctggag taaaaataat aaggcctttg
     1081 tcagtttttg gctacacaga taattttcat ggaggacatt ttgagatcca ttttaatcaa
     1141 gtgcgagttc ctgccacaaa tctaatacta ggtgaaggta ggggatttga aatttcccaa
     1201 ggccgccttg gacctggcag aatccaccac tgtatgagaa cagtaggttt ggcggaacgc
     1261 gctttgcaga tcatgtgtga gcgggcaaca caaaggatag ctttcaagaa gaagttgtat
     1321 gcacatgagg ttgtggctca ctggattgct gaaagccgca ttgccattga gaagatccgc
     1381 ttgttgactc tgaaagctgc tcacagcatg gacactctgg gcagtgctgg cgctaagaaa
     1441 gagattgcaa tgatcaaagt ggctgcccca cgggctgtca gcaaaatcgt tgactgggcc
     1501 atccaggtgt gcggaggtgc tggtgtttcc caggattacc ctctggctaa catgtatgct
     1561 ataacccgag ttttgcgttt agcagatgga cctgacgaag ttcatctttc agcaatcgca
     1621 acaatggagc tgcgggacca agccaaaaga ctgacagcca agatataaca gtaaaggtgg
     1681 atacggatcc gaattcgagc tccgtcgaca agctt
//
