LOCUS       sequence-490664-        9064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-Dendra2 from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9064)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-Dendra2.W
FEATURES             Location/Qualifiers
     source          1..9064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2035..2338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2616..3632
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3556..3578
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3640..3659
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3754..4131
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             4141..4830
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     misc_feature    4915..5503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4968..4988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5386..5397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5590..5823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5895..6016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5932..5951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5986..6005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6056..6191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6211..6230)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6212..6230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6240..6257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6240..6256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6249..6271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6398..6853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6485..6504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6695..6716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6879..6983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6984..7052,7053..7844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7202..7221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8015..8603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8504..8523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8757..8774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8891..8912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8927..8932,8933..8950,8951..8957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8965..8981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8970..8992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8989..9005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8989..9005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9024..9044
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9026..9044
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag aagcttgata attctgcagg aatctagtta
     1981 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2041 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2101 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2161 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2221 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2281 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2341 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     2401 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     2461 gcgcgccrgg gggggggggg gggggggggg gggggggggg ggsgrggsgg rgrggkgsgg
     2521 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2581 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     2641 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     2701 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     2761 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     2821 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     2881 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     2941 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     3001 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     3061 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     3121 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     3181 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     3241 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     3301 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     3361 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     3421 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     3481 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     3541 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     3601 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3661 tgtctcatca ttttggcaaa gaattgattt gataccgcgg gcccgggatc tcgagctcaa
     3721 gcttcgaatt ctgcagtcga cggtaccgcc accatgccag agccagcgaa gtctgctccc
     3781 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
     3841 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
     3901 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac
     3961 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
     4021 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
     4081 cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa ggatcgatcc
     4141 atgaacaccc cgggaattaa cctgatcaag gaggacatgc gcgtgaaggt gcacatggag
     4201 ggcaacgtga acggccacgc cttcgtgatc gagggcgagg gcaagggcaa gccctacgag
     4261 ggcacccaga ccgccaacct gaccgtgaag gagggcgccc ccctgccctt cagctacgac
     4321 atcctgacca ccgccgtgca ctacggcaac cgggtgttca ccaagtaccc cgaggacatc
     4381 cccgactact tcaagcagag cttccccgag ggctacagct gggagcgcac catgaccttc
     4441 gaggacaagg gcatctgcac catccgcagc gacatcagcc tggagggcga ctgcttcttc
     4501 cagaacgtgc gcttcaaggg caccaacttc ccccccaacg gccccgtgat gcagaagaag
     4561 accctgaagt gggagcccag caccgagaag ctgcacgtgc gcgacggcct gctggtgggc
     4621 aacatcaaca tggccctgct gctggagggc ggcggccact acctgtgcga cttcaagacc
     4681 acctacaagg ccaagaaggt ggtgcagctg cccgacgccc acttcgtgga ccaccgcatc
     4741 gagatcctgg gcaacgacag cgactacaac aaggtgaagc tgtacgagca cgccgtggcc
     4801 cgctacagcc ccctgcccag ccaggtgtgg tccggcgaca gcggcgtgta caagtgaaag
     4861 cttaattagc tgagcttgga ctcctgttga aagggcgaat tctgcagatt cgacaatcaa
     4921 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4981 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5041 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5101 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5161 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5221 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5281 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     5341 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5401 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5461 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tcgagctcgg
     5521 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5581 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5641 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5701 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5761 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5821 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5881 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6001 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6121 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6181 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6361 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6841 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8761 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8821 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8881 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8941 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9001 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9061 tgca
//
