LOCUS       sequence-49067-e         409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 409)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACBD4
FEATURES             Location/Qualifiers
     source          1..409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gcccagagcc cgactgccag aaacagttcc aggctgcagt gagcgtcatc
      121 cagaacctgc ccaagaacgg ttcttaccgc ccctcctatg aagagatgct gcgattctac
      181 agttactaca agcaggccac catggggccc tgcctggtcc cccggcccgg gttctgggac
      241 cccattggac gatataagtg ggacgcctgg aacagtctgg gcaagatgag cagggaggag
      301 gccatgtctg cctacatcac tgaaatgaaa ctggtggcac agaaggtgat cgacacagtg
      361 cccctgggtg aggtggcaga gtgacagtaa aggtggatac ggatccgaa
//
