LOCUS       sequence-490672-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Poetur RFP cargo plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ivancic D, Agudelo A, Lindstrom-Vautrin J, Jaraba-Wallace J, Gallo 
            M, Das R, Ragel A, Herrero-Vicente J, Higueras I, Billeci F, 
            Sanvicente-Garcia M, Petazzi P, Ferruz N, Sanchez-Mejias A, Guell M
  TITLE     Discovery and protein language model-guided design of hyperactive 
            transposases.
  JOURNAL   Nat Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4.
  PUBMED    41039042
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4."
COMMENT     Sequence Label: pTIR_Poetur_RFP
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      668..1256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1157..1176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1489..1700
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1713..1981
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2009..2018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2754..2774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2816..2937
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2853..2872)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2907..2926
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3157..3175)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3243..3347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3348..3416,3417..3711,1..497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3566..3585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
       61 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      121 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      181 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      241 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      301 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      361 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      421 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      481 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      541 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      601 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1261 ccagcaacgc ggccttttta cggttcctgg ccttttgctg ccttaaccct tgtgttatgt
     1321 ttcgggtcaa tttgacccgt ttcgattttt gagttgacca aagtgcaggt tattcttatt
     1381 ttttttccat gaaatcttgt gacttttccg catctagggt catgaacttc tgagtaaaat
     1441 ctggagtctt tgtatgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     1501 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     1561 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     1621 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     1681 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     1741 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     1801 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     1861 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     1921 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     1981 ccaagctgtg accggcgcct acgatatcgc caccatggcc agctccgagg atgtcatcaa
     2041 agagtttatg agatttaagg tcaagatgga gggaagcgtc aacggacacg agttcgagat
     2101 tgagggagaa ggagaaggcc ggccttacga gggcacacaa accgctaagc tcaaggtcac
     2161 aaaaggagga cccctcccct tctcctggga tattctgagc cctcagttcc agtacggaag
     2221 caaagcctat gttaaacacc ctgccgacat ccctgactat ctgaagctct ccttccctga
     2281 aggcttcaag tgggagagat tcatgaactt cgaggacgga ggcgtggtga cagtcacaca
     2341 agatagcacc ctccaggacg gagagtttat ttataaggtg aaactcagag gaaccaactt
     2401 cccctccgat ggccctgtca tgcaaaaaaa aacaatggga tgggaagcct ccaccgagag
     2461 aatgtatcct gaggatggcg ctctgaaagg cgaaattaaa atgagactga aactcaaaga
     2521 cggaggacac tacgatgccg aggtcaaaac aacctacaag gccaagaaac aagtgcagct
     2581 gcctggcgcc tacatgactg atattaaact cgacattatc agccataatg gggactacac
     2641 catcgtggaa caatatgaga gagctgaggg cagacatagc acaggcgctg gataagtcga
     2701 caatcaacct ctgattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgcgtt aactaaactt
     2821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     2941 tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctatctg gtctcccttg
     3001 agctctttca taagcgtgta tgtaatctaa aataaagttc tgactggttt gacttcattt
     3061 tttgactgtt ttcagacgga ttacacaata cgggtcaaaa tgacccgcaa catcataaat
     3121 gtaattattt ctcaacataa tacaagggtt aaaggagcct cgtgatacgc ctatttttat
     3181 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3241 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc a
//
