LOCUS       sequence-49069-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             714..722
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             781..801
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             802..819
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             820..843
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggcagcat gccgtgcatt aaaagctgtt ttggtagatc tcagtggcac acttcacatt
       61 gaagatgcag ctgtgccagg cgcacaggaa gctcttaaaa ggttacgtgg tgcttctgta
      121 atcattaggt ttgtgaccaa tacaaccaaa gagagcaagc aagacctgtt agaaaggttg
      181 agaaaattgg aatttgatat ctctgaagat gaaatattca catctctgac tgcagccaga
      241 agtttactag agcggaaaca agtcagaccc atgctgctag ttgatgatcg ggcactacct
      301 gatttcaaag gaatacaaac aagtgatcct aatgctgtgg tcatgggatt ggcaccagaa
      361 cattttcatt atcaaattct gaatcaagca ttccggttac tcctggatgg agcacctctg
      421 atagcaatcc acaaagccag gtattacaag aggaaagatg gcttagccct ggggcctgga
      481 ccatttgtga ctgctttaga gtatgccaca gataccaaag ccacagtcgt ggggaaacca
      541 gagaagacgt tctttttgga agcattgcgg ggcactggct gtgaacctga ggaggctgtc
      601 atgataggag atgattgcag ggatgatgtt ggtggggctc aagatgtcgg catgctgggc
      661 atcttagtaa agactgggaa atatcgagca tcagatgaag aaaaaattaa tccacctcct
      721 tacttaactt gtgagagttt ccctcatgct gtggaccaca ttctgcagca cctattggca
      781 gagaacctct acttccaatc gcaccatcat caccaccatg attacaagga tgacgacgat
      841 aagtgaggat cc
//
