LOCUS       sequence-490708-       22054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant co-editing vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22054)
  AUTHORS   Huang X, Jia H, Xu J, Wang Y, Wen J, Wang N
  TITLE     Transgene-free genome editing of vegetatively propagated and 
            perennial plant species in the T0 generation via a co-editing 
            strategy.
  JOURNAL   Nat Plants. 2023 Oct;9(10):1591-1597. doi: 
            10.1038/s41477-023-01520-y. Epub 2023 Sep 18.
  PUBMED    37723203
REFERENCE   2  (bases 1 to 22054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-023-01520-y"; journalName: "Nat Plants"; date: 
            "2023-10"; volume: "9"; issue: "10"; pages: "1591-1597"
COMMENT     Sequence Label: CBE-Cas12a-GFP
FEATURES             Location/Qualifiers
     source          1..22054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    52..226
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(293..313)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     908..936
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          973..1161
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             4281..4301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5433..5456,5457..5459,5460..9533)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9552..9599
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9621..9869
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9891..9911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9935..10183
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     terminator      10201..10453
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             10968..10988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14694..14741
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      14756..15004
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     15030..15049
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15167..15341
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    15419..15443
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15868..16662
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16749..17337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17238..17257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17491..17508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    17523..17663
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(17589..17608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17749..17771
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      18007..18201
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(18267..19340)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(19769..20398)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    21699..21723
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(21745..21997)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(22032..22049)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgttaacact agtcagatct accatggtgg actcctctta aagcttcggg ggatctggat
       61 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
      121 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
      181 cctaattccc ttatctggga actactcaca cattattatg gagaaatttt cgagctctta
      241 aagctcatca tgtttgtata gttcatccat gccatgtgta atcccagcag ctgttacaaa
      301 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      361 ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg
      421 ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttagc
      481 tttgattcca ttcttttgtt tgtctgccgt gatgtatacg ttgtgggagt tgtagttgta
      541 ttccaacttg tggccgagga tgtttccgtc ctccttgaaa tcgattccct taagctcgat
      601 cctgttgacg agggtgtctc cctcaaactt gacttcagca cgtgtcttgt agttcccgtc
      661 gtccttgaaa gagatggtcc tctcctgcac gtatccctca ggcatggcgc tcttgaagaa
      721 gtcgtgccgc ttcatatgat ctgggtatct tgaaaagcat tgaacaccat aagtgaaagt
      781 agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa atttaagggt
      841 aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt tgtgcccatt
      901 aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt cttctccttt
      961 actgaattgt acctgcacat caacaaattt tggtcatata ttagaaaagt tataaattaa
     1021 aatatacaca cttataaact acagaaaagc aattgctata tactacattc ttttattttg
     1081 aaaaaaatat ttgaaatatt atattactac taattaatga taattattat atatatatca
     1141 aaggtagaag cagaaactta cgtacaattc ggccgaggat aatgatagga gaagtgaaaa
     1201 gatgagaaag agaaaaagat tagtcttcat ctcgacaaac ttacaaattt ctctgaagtt
     1261 gtatcctcag tacttcaaag aaaatagctt acaccaaatt ttttcttgtt ttcacaaatg
     1321 ccgaacttgg ttccttatat aggaaaactc aagggcaaaa atgacacgga aaaatataaa
     1381 aggataagta gtgggggata agattccttt gtgataaggt tactttccgc ccttacattt
     1441 tccaccttac atgtgtcctc tatgtctctt tcacaatcac cgaccttatc ttcttctttt
     1501 cattgttgtc gtcagtgctt acgtcttcaa gattcttttc ttcgcctggt tcttcttttt
     1561 caatttctac gtattcttct tcgtattctg gcagtatagg atcttgtatc tgtacattct
     1621 tcatttttga acataggttg catatgtgcc gcatattgat ctgcttcttg ctgagctcac
     1681 ataatacttc catagttttt cccgtaaaca ttggattctt gatgctacat cttggataat
     1741 taccttctgc agcccttaca ttttccacct tacatgtgtc ctctatgtct ctttcacaat
     1801 caccgacctt atcttcttct tttcattgtt gtcgtcagtg cttacgtctt caagattctt
     1861 ttcttcgcct ggttcttctt tttcaatttc tacgtattct tcttcgtatt ctggcagtat
     1921 aggatcttgt atctgtacat tcttcatttt tgaacatagg ttgcatatgt gccgcatatt
     1981 gatctgcttc ttgctgagct cacataatac ttccatagtt tttcccgtaa acattggatt
     2041 cttgatgcta catcttggat aattaccttc tggcgcgggc atgcaagctt gcatgccgcg
     2101 ctcaggagcc ggttgaattt gattgttgtt tgatgtttag gtatgcttac aattttttac
     2161 taaataaggt aataattgtt ccttttattt tattcattta gcttggtact tgtcgtaatc
     2221 tatctccgtc aatgtcagct tcttctgtgt cggaagaagc tgaaaaatgt tgttgaaggc
     2281 cctttcattt attattatca ttattatgtt taattttgtt cggcaaatgt gtactaattt
     2341 ggttcgaatc gttcgatctc ataatacaaa catgtaatga gttatttatg acgtcatgga
     2401 tgcggttagt ttgcgtggtc tgaaatttca accaaattat tcaatcatgg tggtggccgg
     2461 ctggtgccta tcccttgatt gaaatatgca attaaaattc tcgtaataat gttgatttgt
     2521 tctatatact tgaattgatg gtattaatta ttgttataaa agcactaact tgtttgagaa
     2581 aggggataac taaaaggtaa taataataat aataaaataa agaaaaggcc caacacatgg
     2641 gcgccctatc tgttgggcca aatgccataa gaggatccag ctagcccgtt gaagaaaatc
     2701 ccacatcgaa agaaaaaact gaataaacat ggtctatata tacaaggact cccaggttgg
     2761 ttgaacaaag caccagtggt ctagtggtag aatagtaccc tgccacggta cagacccggg
     2821 ttcgattccc ggctggtgca aaatgcaggt gatgactcac ctgcataagt ttaagagcta
     2881 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     2941 caccgagtcg gtgcaacaaa gcaccagtgg tctagtggta gaatagtacc ctgccacggt
     3001 acagacccgg gttcgattcc cggctggtgc attttttttt cccctgtttt ttcctaatta
     3061 gatttctttc ggagctgttc gaagaacatg tttttgccgt ctgactcgtt tgttattgcc
     3121 gttctagtta gtttcagcta ttggttttcg gtttctttct gttttgaagg gtaatagttt
     3181 ggaaaatata atacaaatta atttaggatt aaaaagtaat aaaacaatat tatccctgca
     3241 ggcgatcggc gcgccagatt tgccttttca atttcagaaa gaatgctaac ccacagatgg
     3301 ttagagaggc ttacgcagca ggtatcatca agacgatcta cccgagcaat aatctccagg
     3361 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
     3421 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
     3481 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
     3541 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
     3601 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
     3661 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
     3721 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggatggcaga
     3781 catactgtcc cacaaatgaa gatggaatct gtaaaagaaa acgcgtgaaa taatgcgtct
     3841 gacaaaggtt aggtcggctg cctttaatca ataccaaagt ggtccctacc acgatggaaa
     3901 aactgtgcag tcggtttggc tttttctgac gaacaaataa gattcgtggc cgacaggtgg
     3961 gggtccacca tgtgaaggca tcttcagact ccaataatgg agcaatgacg taagggctta
     4021 cgaaataagt aagggtagtt tgggaaatgt ccactcaccc gtcagtctat aaatacttag
     4081 cccctccctc attgttaagg gagcaaaatc tcagagagat agtcctagag agagaaagag
     4141 agcaagtagc ctagaagtag tcaaggcggc gaagtattca ggcacgtggc caggaagaag
     4201 aaaagccaag acgacgaaaa caggtaagag ctaagctttc tagaatgaaa agaacagctg
     4261 acggcagtga gttcgagagc cccaagaaga agcgtaaagt tgaggcaagc ccagcttcag
     4321 gccccagaca cctcatggac ccacacatct tcacatcaaa tttcaataat ggaattggga
     4381 gacataaaac atatctatgc tacgaagtgg agcgtctcga taatggcacc agcgtcaaga
     4441 tggaccaaca tcgtggattt ctgcataatc aagccaaaaa cctgctatgt gggttttacg
     4501 gacgacatgc agagttacgt tttcttgacc tcgtgccttc actacagctt gatcccgccc
     4561 aaatatatcg agttacctgg tttatttcct ggtctccatg tttctcttgg gggtgtgctg
     4621 gggaagtccg agccttttta caggagaata cacatgtcag gttgaggatc tttgctgcca
     4681 gaatctatga ttatgatccc ctttacaagg aagcactcca gatgttaaga gacgctggcg
     4741 cacaagtgag cattatgact tatgacgagt tcaaacattg ctgggatacc tttgtcgatc
     4801 atcaaggatg tccttttcag ccttgggacg gtctggacga acattcccag gcattaagcg
     4861 gacgtttgcg tgctatactt caaaatcaag gtaactccgg cgggtcatca ggtggcagca
     4921 gtggatctga gacccccggt acttctgaat cagcaactcc tgaatcctct ggtggctctt
     4981 ctgggggcag cgcaatgcaa atgcagcttg aagcaaacgc agatacttcc gttgaagaag
     5041 agtctttcgg accccagcca atatcccgac ttgagcagtg cggcataaat gctaatgacg
     5101 taaaaaaact ggaggaggct gggttccata ctgtcgaagc agtggcctac gcacctaaga
     5161 aagaattaat aaatatcaag ggcatatcag aggcaaaggc tgacaaaata ctggctgagg
     5221 ccgccaagtt agtccctatg ggatttacca ctgccaccga gttccatcag cgtcgatcag
     5281 agattataca aatcacaact ggatccaaag aactagataa actgttacaa gtcgactccg
     5341 gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac
     5401 ctgaaagcag cgggggcagc agcggggggt cagacaagaa gtacagcatc ggcctggcta
     5461 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga
     5521 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc
     5581 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa
     5641 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg
     5701 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata
     5761 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga
     5821 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc
     5881 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg
     5941 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga
     6001 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta
     6061 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg
     6121 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta
     6181 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct
     6241 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc
     6301 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg
     6361 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg
     6421 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt
     6481 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag
     6541 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg
     6601 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc
     6661 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc
     6721 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact
     6781 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg
     6841 aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt
     6901 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
     6961 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
     7021 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
     7081 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
     7141 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
     7201 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
     7261 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
     7321 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
     7381 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
     7441 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
     7501 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
     7561 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
     7621 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
     7681 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
     7741 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
     7801 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
     7861 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
     7921 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
     7981 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
     8041 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
     8101 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
     8161 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
     8221 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
     8281 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
     8341 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
     8401 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
     8461 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
     8521 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
     8581 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
     8641 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
     8701 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
     8761 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
     8821 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
     8881 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
     8941 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
     9001 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
     9061 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
     9121 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
     9181 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
     9241 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
     9301 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
     9361 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
     9421 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
     9481 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg
     9541 ctgatgaatt caagagaccc gcagcaacca agaaggcagg gcaagcaaag aagaagaaga
     9601 cgcgtgactc cggaggcagc accaacctgt ccgacatcat cgagaaggag acgggcaagc
     9661 aactcgtgat ccaggagagc atcctcatgc tgccagagga ggtggaggag gtcatcggca
     9721 acaagccaga gtccgacatc ctggtgcaca ccgcctacga cgagtccacc gacgagaacg
     9781 tcatgctcct gaccagcgac gccccagagt acaagccatg ggccctcgtc atccaggaca
     9841 gcaacgggga gaacaagatc aagatgctgt cgggggggag ccaattcgag cccaagaaga
     9901 agaggaaagt ctaaccggtc atcatcactc gagatatgaa gatgaagatg aaatatttgg
     9961 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
    10021 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
    10081 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
    10141 gtatgtgcta tggtatggac tatggaatat gattaaagat aaggagcctc gaatttcccc
    10201 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    10261 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    10321 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    10381 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    10441 atgttactag atcggcgcgc cgggccctgt caaacactga tagtttaaac tggcagacat
    10501 actgtcccac aaatgaagat ggaatctgta aaagaaaacg cgtgaaataa tgcgtctgac
    10561 aaaggttagg tcggctgcct ttaatcaata ccaaagtggt ccctaccacg atggaaaaac
    10621 tgtgcagtcg gtttggcttt ttctgacgaa caaataagat tcgtggccga caggtggggg
    10681 tccaccatgt gaaggcatct tcagactcca ataatggagc aatgacgtaa gggcttacga
    10741 aataagtaag ggtagtttgg gaaatgtcca ctcacccgtc agtctataaa tacttagccc
    10801 ctccctcatt gttaagggag caaaatctca gagagatagt cctagagaga gaaagagagc
    10861 aagtagccta gaagtagtca aggcggcgaa gtattcaggc acgtggccag gaagaagaaa
    10921 agccaagacg acgaaaacag gtaagagcta agcttcctag gatggctcct aagaagaagc
    10981 ggaaggttgg tattcacggg gtgcctgcgg cttcaaagct cgagaaattc accaactgtt
    11041 attcgttgag caaaacactg cggtttaaag cgattccagt cggcaagact caagagaata
    11101 tagacaataa gcggctgttg gtggaagatg aaaagcgcgc ggaagactac aaaggggtga
    11161 agaagttgtt ggacagatac tacctctctt ttatcaatga tgtcttgcac tcaatcaaat
    11221 tgaagaatct gaacaactac atctccctct tcagaaagaa aacaaggaca gaaaaggaga
    11281 ataaggaact tgaaaatttg gagatcaatc tgaggaaaga gatcgcgaaa gcctttaaag
    11341 gcaacgaagg atacaaaagt ctgttcaaga aggatataat tgagacaatt ttgccagagt
    11401 tcctcgatga caaggacgag attgcgctgg tcaattcgtt caacggattc acaacagcat
    11461 tcacaggctt ctttcggaat cgggaaaata tgttctctga ggaggcaaag tccacttcta
    11521 ttgcgttcag gtgtatcaat gagaatctca ctaggtacat ttccaacatg gatatctttg
    11581 agaaggttga cgcaattttt gacaagcacg aagttcagga gattaaggag aagatcctca
    11641 attccgatta tgacgttgag gacttcttcg aaggtgagtt ttttaatttc gtgctcactc
    11701 aagagggtat cgacgtgtat aatgcgatca tcggtgggtt cgtgactgag tccggtgaaa
    11761 agattaaggg attgaacgag tatatcaacc tttacaacca aaagacgaaa cagaagctgc
    11821 caaagttcaa gcctctttac aaacaggttc tttcagaccg cgagtcactc tcgttctatg
    11881 gggagggcta cacttcggat gaggaagtcc tggaggtgtt caggaatact ctcaataaga
    11941 attcggagat tttctcttct ataaaaaaac tggaaaagtt gtttaagaat tttgacgaat
    12001 actctagcgc cggcatattt gtgaaaaacg gcccggccat atcaacgata agtaaagata
    12061 tcttcggcga atggaacgtg atcagagaca aatggaacgc ggagtatgac gatattcacc
    12121 tgaagaagaa ggctgtcgta acggagaagt acgaggatga tcgcaggaaa agcttcaaaa
    12181 agatcggaag tttcagcctg gaacagttgc aggagtatgc tgacgccgat cttagcgtcg
    12241 tcgagaagtt gaaggagata atcatccaaa aggtcgacga gatatataaa gtctatggat
    12301 caagtgaaaa actgttcgac gccgacttcg ttttggagaa gtccctgaag aagaacgacg
    12361 ctgttgttgc cattatgaag gatctgctcg acagcgtgaa gagtttcgag aactatatta
    12421 aggctttttt cggggagggg aaggagacta acagagatga gtccttctac ggagacttcg
    12481 tcctcgcgta cgatatactc cttaaggtag accacatcta cgacgcaatc agaaattacg
    12541 tgacacaaaa gccgtacagc aaggacaagt tcaaactcta cttccagaac ccccagttca
    12601 tgggcggctg ggacaaggac aaggaaacgg attacagggc tacgatcctg aggtatggtt
    12661 caaaatacta cttggcgatt atggacaaga agtacgccaa gtgtctccag aagattgaca
    12721 aagacgatgt caatggcaat tatgagaaga tcaactacaa gctgcttccg ggtccgaaca
    12781 agatgctccc aaaggttttc ttcagcaaga aatggatggc atactataac ccaagcgagg
    12841 acatccagaa gatttataag aacggtacgt tcaagaaggg cgacatgttc aatcttaacg
    12901 actgtcacaa gctgatcgac ttcttcaaag actcaattag ccggtaccca aagtggtcta
    12961 acgcctatga cttcaacttt tcggaaaccg agaagtacaa ggatatagcc ggattttata
    13021 gagaggtgga agagcagggc tacaaggtgt cattcgagtc cgccagcaag aaggaagtgg
    13081 acaagctcgt ggaagagggt aagctctaca tgttccagat ttataataaa gactttagcg
    13141 ataagagcca cgggacacct aatctccaca caatgtattt caagctgctc ttcgacgaga
    13201 ataaccacgg ccaaatcagg ttgtcaggag gggctgaact cttcatgcgg cgcgctagcc
    13261 ttaagaagga ggagcttgta gtccaccctg cgaatagtcc aattgcgaat aagaacccgg
    13321 acaatcctaa aaagactaca acattgagct acgacgtgta caaggataag aggttttccg
    13381 aggatcagta cgagctccac atcccgattg cgatcaacaa gtgcccaaag aatattttca
    13441 agataaacac agaggtgcgt gtactcctga agcatgacga caatccttac gtcattggga
    13501 ttgatcgggg cgagaggaac ctcctctata ttgtggtggt ggacgggaag gggaacatag
    13561 tcgaacagta ctcccttaac gaaataatta acaatttcaa cggcatccgt atcaagaccg
    13621 actaccattc gttgctggac aagaaggaga aggagagatt tgaggcgcgg caaaattgga
    13681 caagtatcga gaacatcaag gaactcaaag caggttatat ctctcaagtt gtgcataaga
    13741 tatgcgagct ggttgagaag tatgacgcag tgatcgctct tgaggacctc aactcgggct
    13801 ttaagaattc tagagttaaa gtggagaagc aggtctatca aaagttcgag aagatgctta
    13861 tagataagct caactacatg gtcgataaga aatcgaaccc atgtgccacc ggcggcgcac
    13921 tcaaaggtta ccaaataaca aacaaattcg agtccttcaa atcgatgagt actcagaatg
    13981 ggttcatatt ttatataccg gcgtggctta cgtctaagat cgacccgtca actggttttg
    14041 tcaacctgtt gaagacgaaa tacacgtcca ttgccgattc gaaaaagttc atatctagtt
    14101 ttgatcgtat tatgtacgtc ccagaggaag atcttttcga gtttgctctc gactacaaaa
    14161 acttttcgcg gaccgatgcg gattacatta aaaaatggaa actctattcg tacggcaaca
    14221 gaatcaggat ttttcgcaac cctaagaaga ataacgtctt tgattgggag gaagtttgct
    14281 tgactagcgc gtacaaggag ctctttaata agtatggcat taactaccaa cagggtgata
    14341 tcagagcact gctttgcgaa caatctgaca aggctttcta ctcatccttc atggctttga
    14401 tgagcctgat gctccagatg agaaattcaa ttacaggcag aaccgacgtg gatttcttga
    14461 tctccccggt taaaaattct gatggcatct tttacgatag caggaactat gaagcgcaag
    14521 agaatgcgat tctgccaaaa aatgcagacg ccaacggtgc ctataacatc gccaggaaag
    14581 tcctgtgggc gatcggccag ttcaaaaagg ccgaagacga aaaattggac aaggtcaaaa
    14641 tcgctatcag caacaaagag tggctggagt atgctcagac atccgtaaag cataagcgtc
    14701 ctgctgccac caaaaaggcc ggacaggcta agaaaaagaa gtgagacgac tagtatatga
    14761 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
    14821 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
    14881 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
    14941 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
    15001 taaggtggct gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
    15061 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
    15121 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctaa gtcgagtttc tccataataa
    15181 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
    15241 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
    15301 tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt
    15361 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
    15421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
    15481 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
    15541 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
    15601 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
    15661 gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata
    15721 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
    15781 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
    15841 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
    15901 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
    15961 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
    16021 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
    16081 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
    16141 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
    16201 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
    16261 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
    16321 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
    16381 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
    16441 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
    16501 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
    16561 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
    16621 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc
    16681 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    16741 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    16801 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    16861 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    16921 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    16981 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    17041 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    17101 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    17161 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    17221 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    17281 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    17341 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    17401 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    17461 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    17521 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
    17581 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
    17641 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
    17701 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    17761 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
    17821 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
    17881 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
    17941 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
    18001 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
    18061 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
    18121 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
    18181 ccaatgtacg tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca
    18241 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
    18301 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
    18361 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
    18421 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    18481 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    18541 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    18601 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    18661 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    18721 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    18781 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    18841 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    18901 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    18961 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    19021 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    19081 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    19141 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    19201 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    19261 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    19321 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    19381 gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    19441 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    19501 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    19561 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    19621 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    19681 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    19741 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    19801 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    19861 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    19921 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    19981 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    20041 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    20101 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    20161 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    20221 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    20281 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    20341 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    20401 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    20461 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    20521 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    20581 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    20641 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    20701 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    20761 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    20821 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    20881 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    20941 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    21001 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    21061 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    21121 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    21181 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    21241 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    21301 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    21361 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    21421 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    21481 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    21541 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    21601 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
    21661 aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
    21721 tcaaacactg atagtttaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt
    21781 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
    21841 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
    21901 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
    21961 atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcgag ctggtcacct
    22021 gtaattcaca cgtggtggtg gtggtggtgg ctag
//
