LOCUS       sequence-490712-        1584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SNAP-Rab6a (WT) in mammalian cells. Generated from Addgene
            #46781 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels
            B, Luxardi G, Moshiri A, Deng WT, Robichaux MA
  TITLE     Super-resolution microscopy reveals a Rab6a-dependent trafficking 
            hub for rhodopsin at the mammalian rod photoreceptor Golgi.
  JOURNAL   bioRxiv [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 
            10.1101/2025.09.24.678292.
  PUBMED    41040232
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 
            10.1101/2025.09.24.678292."
COMMENT     Sequence Label: pCMV-SNAP-Rab6a
FEATURES             Location/Qualifiers
     source          1..1584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          159..291
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     274..293
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        336..354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             366..911
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc
       61 taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa
      121 ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
      181 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
      241 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
      301 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
      361 tcgacatgga caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc
      421 tggaactgtc tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat
      481 ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac
      541 tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt
      601 tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc
      661 tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg
      721 ccctggccgg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg
      781 tgcccattct gatcccctgc caccgggtgg tgcagggcga cctggacgtg gggggctacg
      841 agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc
      901 ctgggctggg ttctagagga tccaccatgt ccacgggcgg agacttcggg aatccgctga
      961 ggaaattcaa gctggtgttc ctgggggagc aaagcgttgg aaagacatct ttgatcacca
     1021 gattcatgta tgacagtttt gacaacacct atcaggcaac aattggcatt gactttttat
     1081 caaaaactat gtacttggag gatcgaacag tacgattgca attatgggac acagcaggtc
     1141 aagagcggtt caggagcttg attcctagct acattcgtga ctccactgtg gcagttgttg
     1201 tttatgatat cacaaatgtt aactcattcc agcaaactac aaagtggatt gatgatgtca
     1261 gaacagaaag aggaagtgat gttatcatca tgctagtagg aaataaaaca gatcttgctg
     1321 acaagaggca agtgtcaatt gaggagggag agaggaaagc caaagagctg aatgttatgt
     1381 ttattgaaac tagtgcaaaa gctggataca atgtaaagca gctctttcga cgtgtagcag
     1441 cagctttgcc gggaatggaa agcacacagg acagaagcag agaagatatg attgacataa
     1501 aactggaaaa gcctcaggag caaccagtca gtgaaggagg ctgttcctgc taactcgagc
     1561 atgcatctag agggccctat tcta
//
