LOCUS       sequence-490716-        1600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SNAP-Rab6a-T27N (dominant negative) in mammalian cells. 
            Generated from Addgene #46782 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Hekmatara M, Thompson SL, Haggerty KN, Hagen S, Brothers BA, Daniels
            B, Luxardi G, Moshiri A, Deng WT, Robichaux MA
  TITLE     Super-resolution microscopy reveals a Rab6a-dependent trafficking 
            hub for rhodopsin at the mammalian rod photoreceptor Golgi.
  JOURNAL   bioRxiv [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 
            10.1101/2025.09.24.678292.
  PUBMED    41040232
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Sep 25:2025.09.24.678292. doi: 
            10.1101/2025.09.24.678292."
COMMENT     Sequence Label: pCMV-SNAP-Rab6a_T27N
FEATURES             Location/Qualifiers
     source          1..1600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          166..298
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     281..300
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        343..361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             373..918
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagtgact
      121 ctcttaaggt agccttgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt
      181 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
      241 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
      301 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag
      361 gctagcctcg acatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg
      421 ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa
      481 ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca
      541 gagccactga tgcaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc
      601 gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc
      661 caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag ctacagccac
      721 ctggccgccc tggccggcaa tcccgccgcc accgccgccg tgaaaaccgc cctgagcgga
      781 aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacct ggacgtgggg
      841 ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg
      901 ggcaagcctg ggctgggttc tagaggatcc accatgtcca cgggcggaga cttcgggaat
      961 ccgctgagga aattcaagct ggtgttcctg ggggagcaaa gcgttggaaa gaattctttg
     1021 atcaccagat tcatgtatga cagttttgac aacacctatc aggcaacaat tggcattgac
     1081 tttttatcaa aaactatgta cttggaggat cgaacagtac gattgcaatt atgggacaca
     1141 gcaggtcaag agcggttcag gagcttgatt cctagctaca ttcgtgactc cactgtggca
     1201 gttgttgttt atgatatcac aaatgttaac tcattccagc aaactacaaa gtggattgat
     1261 gatgtcagaa cagaaagagg aagtgatgtt atcatcatgc tagtaggaaa taaaacagat
     1321 cttgctgaca agaggcaagt gtcaattgag gagggagaga ggaaagccaa agagctgaat
     1381 gttatgttta ttgaaactag tgcaaaagct ggatacaatg taaagcagct ctttcgacgt
     1441 gtagcagcag ctttgccggg aatggaaagc acacaggaca gaagcagaga agatatgatt
     1501 gacataaaac tggaaaagcc tcaggagcaa ccagtcagtg aaggaggctg ttcctgctaa
     1561 ctcgagcatg catctagagg gccctattct atagtgtcac
//
