LOCUS       sequence-49073-e         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTS
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gccgtcgctg ccaggcacaa gtgtcccgcc gcatctcctt cagcgcgagc
      121 caccgattgt acagtaaatt tctaagtgat gaagaaaact tgaaactgtt tgggaaatgc
      181 aacaatccaa atggccatgg gcacaattat aaagttgtgg tgacagtaca tggagagatt
      241 gaccctgcta cgggaatggt tatgaatctg gctgatctca aaaaatatat ggaggaggcg
      301 attatgcagc cccttgatca taagaatctg gatatggatg tgccatactt tgcagatgtg
      361 gtgagcacga ctgaaaatgt agctgtttat atctgggaca acctccagaa agttcttcct
      421 gtaggagttc tttataaagt aaaagtatac gaaactgaca ataatattgt ggtttataaa
      481 ggagaatgac agtaaaggtg gatacggatc cgaa
//
