LOCUS       sequence-490748-       10112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CaMV 35S driving the expression of dCas9-ZAT10(2x).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, 
            Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, 
            Secco D, Small I, Kidd BN, Lister R
  TITLE     CRISPRi-based circuits to control gene expression in plants.
  JOURNAL   Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 
            10.1038/s41587-024-02236-w. Epub 2024 May 20.
  PUBMED    38769424
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-024-02236-w"; journalName: "Nat Biotechnol"; date: 
            "2025-03"; volume: "43"; issue: "3"; pages: "416-430"
COMMENT     Sequence Label: LLP1016
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1224..1244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1248..1268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1662..1914
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(1939..1957)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1978..1994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1978..1994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1991..2013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2002..2018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2026..2032,2033..2050,2051..2056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2071..2092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2209..2226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2380..2968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2460..2479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3139..3930,3931..3999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3762..3781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4000..4104)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4130..4585)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4267..4288)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4479..4498
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4712..4734
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4726..4743
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4727..4743
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4750..4769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4750..4768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4804..5056
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      5086..5286
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     CDS             5652..5672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6478..6526
                     /note="synthetic polyadenylation signal"
     misc_feature    6540..6631
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6580..6599
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        6638..6982
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6926..6946
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6998..7018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7034..8470,8660..10112,1..1211)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          8471..8659
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 atttcagaaa ggatttccag ttctacaagg ttagagagat caacaactac caccacgctc
       61 acgatgctta ccttaacgct gttgttggaa ccgctcttat caagaagtac ccaaagttgg
      121 agtctgagtt cgtttacgga gattacaagg tttacgatgt tagaaagatg atcgctaagt
      181 ctgagcagga gatcggaaag gctaccgcta agtacttctt ctactctaac atcatgaact
      241 tcttcaagac cgagatcacc cttgctaacg gagagatcag aaagagacca cttatcgaga
      301 ccaacggaga gaccggagag atcgtttggg ataagggaag agatttcgct accgttagaa
      361 aggttctttc tatgccacag gttaacatcg ttaagaaaac cgaggttcag accggaggat
      421 tctctaagga gtctatcctt ccaaagagaa actctgataa gttgatcgct agaaagaagg
      481 attgggaccc aaagaagtac ggaggattcg attctccaac cgttgcttac tctgttcttg
      541 ttgttgctaa ggttgagaag ggaaagtcta agaagttgaa gtctgttaag gagcttcttg
      601 gaatcaccat catggagcgt tcttctttcg agaagaaccc aatcgatttc cttgaggcta
      661 agggatacaa ggaggttaag aaggatctta tcatcaagtt gccaaagtac tctcttttcg
      721 agcttgagaa cggaagaaag agaatgcttg cttctgctgg agagcttcag aagggaaacg
      781 agcttgctct tccatctaag tacgttaact tcctttacct tgcttctcac tacgagaagt
      841 tgaagggatc tccagaggat aacgagcaga agcagctttt cgttgagcag cacaagcact
      901 accttgatga gatcatcgag caaatctctg agttctctaa gagagttatc cttgctgatg
      961 ctaaccttga taaggttctt tctgcttaca acaagcacag agataagcca atcagagagc
     1021 aggctgagaa catcatccac cttttcaccc ttaccaacct tggtgctcca gctgctttca
     1081 agtacttcga taccaccatc gatagaaaaa gatacacctc taccaaggag gttcttgatg
     1141 ctacccttat ccaccagtct atcaccggac tttacgagac cagaatcgat ctttctcagc
     1201 ttggaggaga tgaaggagct gatcctaaga agaaaagaaa ggttgatcca aagaaaaaga
     1261 ggaaagttgg tggaggtgga tcaggaggtg gaggatctgg tgggggagga tccgaaggag
     1321 ctggatctac ttctcatgtt tcttcttctc ataggggatt tgaccttaac attccaccaa
     1381 tccctgaatt ttctatggtt aacggagacg acgaggttat gtctccaatg cctgctaaaa
     1441 aacctagatt cgattttcca gttaaacttc agcttggagg tggtggttca gagggagcag
     1501 gatcaacttc acatgtgtct tcatctcaca gaggttttga ccttaacatt ccacctatcc
     1561 ctgagttttc aatggtgaat ggtgatgacg aagtgatgtc acctatgcca gctaaaaagc
     1621 caagattcga tttccctgtg aagttgcaat tgtgagtcga cgatcgttca aacatttggc
     1681 aataaagttt cttaagattg aatcctgttg ccggtcttgc gacgattatc atataatttc
     1741 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     1801 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     1861 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcagtcac
     1921 tcgagccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     1981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     4141 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4201 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4261 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4321 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4381 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4441 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4501 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4561 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     4621 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4681 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4741 agtgaattgt aatacgactc actatagggc gaattgggta caagcttatc taacgatgag
     4801 ctcgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     4861 gcgacgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     4921 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     4981 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     5041 tctatgttac tagatcctgc agactgacta actaggatga gctaagctag ctatatcatc
     5101 aatttatgta ttacacataa tatcgcactc agtctttcat ctacggcaat gtaccagctg
     5161 atataatcag ttattgaaat atttctgaat ttaaacttgc atcaataaat ttatgttttt
     5221 gcttggacta taatacctga cttgttattt tatcaataaa tatttaaact atatttcttt
     5281 caagatggga attaacatct acaaattgcc ttttcttatc gaccatgtac catatgccaa
     5341 cactcgaatc cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt
     5401 tcctttattt aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca
     5461 aaattgtaat ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact
     5521 ataaatacca aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc
     5581 aaccttcgat tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgaccc
     5641 ctaggatggc tccaaaaaag aaaagaaagg ttgctagcga ccattactta gatattagat
     5701 tgagaccaga cccagagttc cctccagcac agttgatgtc agtgttattc ggaaaattgc
     5761 accaggcttt ggttgcacaa ggtggagata gaattggtgt gagttttcct gatttggacg
     5821 agtctaggtc aagattggga gaaaggttaa gaatccatgc ttcagcagat gaccttaggg
     5881 ctttgttagc aagaccttgg ttagagggtc ttagagatca tttgcagttc ggagaaccag
     5941 ctgttgtgcc acaccctaca ccatatagac aagtttctag agtgcaggca aaatcaaatc
     6001 ctgagagact tagaaggaga ttgatgagga gacacgactt atctgaagag gaagctagga
     6061 agagaatacc tgataccgtt gctagagcac ttgacttgcc atttgtgact ttgagaagtc
     6121 aatccactgg tcagcatttt aggttattca ttagacacgg accacttcaa gttactgctg
     6181 aggagggagg attcacctgt tatggattga gtaagggagg attcgtgcct tggttctgag
     6241 gtacccggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc
     6301 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     6361 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     6421 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tactagaaat
     6481 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     6541 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     6601 agtgcaagtg caggtgccag aacatttctc tgaattctga gacttttcaa caaagggtaa
     6661 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     6721 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     6781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     6841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     6901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     6961 gttcatttca tttggagagg acggcactag tgatatgcca aagaagaaga gaaaggtcgg
     7021 aggaggagga tctgacaaga agtactctat cggacttgcc atcggaacca actctgttgg
     7081 atgggctgtt atcaccgatg agtacaaggt tccatctaag aagttcaagg ttcttggaaa
     7141 caccgataga cactctatca agaagaacct tatcggtgct cttcttttcg attctggaga
     7201 gaccgctgag gctaccagat tgaagagaac cgctagaaga agatacacca gaagaaagaa
     7261 cagaatctgc taccttcagg aaatcttctc taacgagatg gctaaggttg atgattcttt
     7321 cttccacaga cttgaggagt ctttccttgt tgaggaggat aagaagcacg agagacaccc
     7381 aatcttcgga aacatcgttg atgaggttgc ttaccacgag aagtacccaa ccatctacca
     7441 ccttagaaag aagttggttg attctaccga taaggctgat cttagactta tctaccttgc
     7501 tcttgctcac atgatcaagt tcagaggaca cttccttatc gagggagacc ttaacccaga
     7561 taactctgat gttgataagt tgttcatcca gcttgttcag acctacaacc agcttttcga
     7621 ggagaaccca atcaacgctt ctggagttga tgctaaggct atcctttctg ctagactttc
     7681 taagtctcgt agacttgaga accttatcgc tcagcttcca ggagagaaga agaacggact
     7741 tttcggaaac cttatcgctc tttctcttgg acttacccca aacttcaagt ctaacttcga
     7801 tcttgctgag gatgctaagt tgcagctttc taaggatacc tacgatgatg atcttgataa
     7861 ccttcttgct cagatcggag atcagtacgc tgatcttttc cttgctgcta agaacctttc
     7921 tgatgctatc cttctttctg acatccttag agttaacacc gagatcacca aggctccact
     7981 ttctgcttct atgatcaaga gatacgatga gcaccaccag gatcttaccc ttttgaaggc
     8041 tcttgttaga cagcagcttc cagagaagta caaggaaatc ttcttcgatc agtctaagaa
     8101 cggatacgct ggatacatcg atggaggagc ttctcaggag gagttctaca agttcatcaa
     8161 gccaatcctt gagaagatgg atggaaccga ggagcttctt gttaagttga acagagagga
     8221 tcttcttaga aagcagagaa ccttcgataa cggatctatc ccacaccaga tccaccttgg
     8281 agagcttcac gctatccttc gtagacagga ggatttctac ccattcttga aggataacag
     8341 agagaagatc gagaagatcc ttaccttcag aatcccatac tacgttggac cacttgctag
     8401 aggaaactct cgtttcgctt ggatgaccag aaagtctgag gagaccatca ccccttggaa
     8461 cttcgaggag gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt
     8521 agtagtaata taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat
     8581 tgcttttctg tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc
     8641 aaaatttgtt gatgtgcagg ttgttgataa gggagcttct gctcagtctt tcatcgagag
     8701 aatgaccaac ttcgataaga accttccaaa cgagaaggtt cttccaaagc actctcttct
     8761 ttacgagtac ttcaccgttt acaacgagct taccaaggtt aagtacgtta ccgagggaat
     8821 gagaaagcca gctttccttt ctggagagca gaagaaggct atcgttgatc ttcttttcaa
     8881 gaccaacaga aaggttaccg ttaagcagtt gaaggaggat tacttcaaga agatcgagtg
     8941 cttcgattct gttgaaatct ctggagttga ggatagattc aacgcttctc ttggaaccta
     9001 ccacgatctt ttgaagatca tcaaggataa ggatttcctt gataacgagg agaacgagga
     9061 catccttgag gacatcgttc ttacccttac ccttttcgag gatagagaga tgatcgagga
     9121 gagactcaag acctacgctc accttttcga tgataaggtt atgaagcagt tgaagagaag
     9181 aagatacacc ggatggggta gactttctcg taagttgatc aacggaatca gagataagca
     9241 gtctggaaag accatccttg atttcttgaa gtctgatgga ttcgctaaca gaaacttcat
     9301 gcagcttatc cacgatgatt ctcttacctt caaggaggac atccagaagg ctcaggtttc
     9361 tggacaggga gattctcttc acgagcacat cgctaacctt gctggatctc cagctatcaa
     9421 gaagggaatc cttcagaccg ttaaggttgt tgatgagctt gttaaggtta tgggtagaca
     9481 caagccagag aacatcgtta tcgagatggc tagagagaac cagaccaccc agaagggaca
     9541 gaagaactct cgtgagagaa tgaagagaat cgaggaggga atcaaggagc ttggatctca
     9601 aatcttgaag gagcacccag ttgagaacac ccagcttcag aacgagaagt tgtaccttta
     9661 ctaccttcag aacggaagag atatgtacgt tgatcaggag cttgacatca acagactttc
     9721 tgattacgat gttgatgcta tcgttccaca gtctttcttg aaggatgatt ctatcgataa
     9781 caaggttctt acccgttctg ataagaacag aggaaagtct gataacgttc catctgagga
     9841 ggttgttaag aagatgaaga actactggag acagcttctt aacgctaagt tgatcaccca
     9901 gagaaagttc gataacctta ccaaggctga gagaggagga ctttctgagc ttgataaggc
     9961 tggattcatc aagagacagc ttgttgagac cagacagatc accaagcacg ttgctcagat
    10021 ccttgattct cgtatgaaca ccaagtacga tgagaacgat aagttgatca gagaggttaa
    10081 ggttatcacc ttgaagtcta agttggtttc tg
//
