LOCUS       sequence-49078-e        1155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1155)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ADA
FEATURES             Location/Qualifiers
     source          1..1155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1084..1104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1105..1122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1123..1146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcccgcct tcgacaagcc caaagtagaa ctgcatgtcc acctagacgg atccatcaag
       61 cctgaaacca tcttatacta tggcaggagg agagggatcg ccctcccagc taacacagca
      121 gaggggctgc tgaacgtcat tggcatggac aagccgctca cccttccaga cttcctggcc
      181 aagtttgact actacatgcc tgctatcgcg ggctgccggg aggctatcaa aaggatcgcc
      241 tatgagtttg tagagatgaa ggccaaagag ggcgtggtgt atgtggaggt gcggtacagt
      301 ccgcacctgc tggccaactc caaagtggag ccaatcccct ggaaccaggc tgaaggggac
      361 ctcaccccag acgaggtggt ggccctagtg ggccagggcc tgcaggaggg ggagcgagac
      421 ttcggggtca aggcccggtc catcctgtgc tgcatgcgcc accagcccaa ctggtccccc
      481 aaggtggtgg agctgtgtaa gaagtaccag cagcagaccg tggtagccat tgacctggct
      541 ggagatgaga ccatcccagg aagcagcctc ttgcctggac atgtccaggc ctaccaggag
      601 gctgtgaaga gcggcattca ccgtactgtc cacgccgggg aggtgggctc ggccgaagta
      661 gtaaaagagg ctgtggacat actcaagaca gagcggctgg gacacggcta ccacaccctg
      721 gaagaccagg ccctttataa caggctgcgg caggaaaaca tgcacttcga gatctgcccc
      781 tggtccagct acctcactgg tgcctggaag ccggacacgg agcatgcagt cattcggctc
      841 aaaaatgacc aggctaacta ctcgctcaac acagatgacc cgctcatctt caagtccacc
      901 ctggacactg attaccagat gaccaaacgg gacatgggct ttactgaaga ggagtttaaa
      961 aggctgaaca tcaatgcggc caaatctagt ttcctcccag aagatgaaaa gagggagctt
     1021 ctcgacctgc tctataaagc ctatgggatg ccaccttcag cctctgcagg gcagaacctc
     1081 gcagagaacc tctacttcca atcgcaccat catcaccacc atgattacaa ggatgacgac
     1141 gataagtgag gatcc
//
