LOCUS       sequence-49079-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RPS6KA1
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg tttttagtga cggctacgtg gtaaaggaga caattggtgt gggctcctac
      121 tctgagtgca agcgctgtgt ccacaaggcc accaacatgg agtatgctgt caaggtcatt
      181 gataagagca agcgggatcc ttcagaagag attgagattc ttctgcggta tggccagcac
      241 cccaacatca tcactctgaa agatgtgtat gatgatggca aacacgtgta cctggtgaca
      301 gagctgatgc ggggtgggga gctgctggac aagatcctgc ggcagaagtt cttctcagag
      361 cgggaggcca gctttgtcct gcacaccatt ggcaaaactg tggagtatct gcactcacag
      421 ggggttgtgc acagggacct gaagcccagc aacatcctgt atgtggacga gtccgggaat
      481 cccgagtgcc tgcgcatctg tgactttggt tttgccaaac agctgcgggc tgagaatggg
      541 ctcctcatga caccttgcta cacagccaac tttgtggcgc ctgaggtgct gaagcgccag
      601 ggctacgatg aaggctgcga catctggagc ctgggcattc tgctgtacac catgctggca
      661 ggatatactc catttgccaa cggtcccagt gacacaccag aggaaatcct aacccggatc
      721 ggcagtggga agtttaccct cagtggggga aattggaaca cagtttcaga gacagccaag
      781 gacctggtgt ccaagatgct acacgtggat ccccaccagc gcctcacagc taagcaggtt
      841 ctgcagcatc catgggtcac ccagaaagac aagcttcccc aaagccagct gtcccaccag
      901 gacctacagc ttgtgaaggg agccatggct gccacgtact ccgcactcaa cagctccaag
      961 cccacccccc agctgaagcc catcgagtca tccatcctgg cccagcggcg agtgaggaag
     1021 ttgccatcca ccaccctgtg acagtaaagg tggatacgga tccgaa
//
