LOCUS       sequence-49080-e        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GOT1
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1204..1224
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1225..1242
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1243..1266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagcctg tcctggtctt caagctcact gccgacttca gggaggatcc ggacccccgc
       61 aaggtcaacc tgggagtggg agcatatcgc acggatgact gccatccctg ggttttgcca
      121 gtagtgaaga aagtggagca gaagattgct aatgacaata gcctaaatca cgagtatctg
      181 ccaatcctgg gcctggctga gttccggagc tgtgcttctc gtcttgccct tggggatgac
      241 agcccagcac tcaaggagaa gcgggtagga ggtgtgcaat ctttgggggg aacaggtgca
      301 cttcgaattg gagctgattt cttagcgcgt tggtacaatg gaacaaacaa caagaacaca
      361 cctgtctatg tgtcctcacc aacctgggag aatcacaatg ctgtgttttc cgctgctggt
      421 tttaaagaca ttcggtccta tcgctactgg gatgcagaga agagaggatt ggacctccag
      481 ggcttcctga atgatctgga gaatgctcct gagttctcca ttgttgtcct ccacgcctgt
      541 gcacacaacc caactgggat tgacccaact ccggagcagt ggaagcagat tgcttctgtc
      601 atgaagcacc ggtttctgtt ccccttcttt gactcagcct atcagggctt cgcatctgga
      661 aacctggaga gagatgcctg ggccattcgc tattttgtgt ctgaaggctt cgagttcttc
      721 tgtgcccagt ccttctccaa gaacttcggg ctctacaatg agagagtcgg gaatctgact
      781 gtggttggaa aagaacctga gagcatcctg caagtccttt cccagatgga gaagatcgtg
      841 cggattactt ggtccaatcc ccccgcccag ggagcacgaa ttgtggccag caccctctct
      901 aaccctgagc tctttgagga atggacaggt aatgtgaaga caatggctga ccggattctg
      961 accatgagat ctgaactcag ggcacgacta gaagccctca aaacccctgg gacctggaac
     1021 cacatcactg atcaaattgg catgttcagc ttcactgggt tgaaccccaa gcaggttgag
     1081 tatctggtca atgaaaagca catctacctg ctgccaagtg gtcgaatcaa cgtgagtggc
     1141 ttaaccacca aaaatctaga ttacgtggcc acctccatcc atgaagcagt caccaaaatc
     1201 gcagagaacc tctacttcca atcgcaccat catcaccacc atgattacaa ggatgacgac
     1261 gataagtgag gatcc
//
