LOCUS       sequence-49081-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KLHL7
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gcacaagacc tagaagaaag aaacatgact accgcatagc cctatttgga
      121 ggctctcaac cacagtcttg tagatatttt aacccaaagg attatagctg gacagacatc
      181 cgctgcccct ttgaaaaacg aagagatgca gcatgcgtgt tttgggacaa tgtagtatac
      241 attttgggag gctctcagct tttcccaata aagcgaatgg actgctataa tgtagtgaag
      301 gatagctggt attcgaaact gggtcctccg acacctcgag acagccttgc tgcatgtgct
      361 gcagaaggca aaatttatac atctggaggt tcagaagtag gaaactcagc tctgtattta
      421 tttgagtgct atgatacgag aactgaaagc tggcacacaa agcccagcat gctgacccag
      481 cgctgcagcc atgggatggt ggaagccaat ggcctaatct atgtttgtgg tggaagttta
      541 ggaaacaatg tttctgggag agtgcttaat tcctgtgaag tttatgatcc tgccacagaa
      601 acatggactg agctgtgtcc aatgattgaa gccaggaaga atcatgggct ggtatttgta
      661 aaagacaaga tatttgctgt gggtggtcag aatggtttag gtggtctgga caatgtggaa
      721 tattacgata ttaagttgaa cgaatggaag atggtctcac caatgccatg gaagggtgta
      781 acagtgaaat gtgcagcagt tggctctata gtttatgtct tggctggttt tcagggtgtt
      841 ggtcgattag gacacattct cgaatataat accgaaacag acaaatgggt tgccaactcc
      901 aaagttcgtg cttttccagt cacaagttgt ttaatttgtg ttgtcgatac ttgtggagca
      961 aatgaagaga cccttgaaac atgacagtaa aggtggatac ggatccgaa
//
