LOCUS       sequence-49084-e        1781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: UGDH
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..129
                     /codon_start=1
                     /product="solubility-enhancement tag derived from T7 phage 
                     gene 10B"
                     /label=SET1
                     /translation="DPEEASVTSTEETLTPAQEAARTRAANKARKEAELAAATAEQ"
     CDS             136..246
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             277..297
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1761..1778
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaccccg aagaggcgag tgttacttca acagaagaaa ccttaacgcc agcacaggag
       61 gccgcacgca cccgcgctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      121 gctgaacaaa ctagtgacga gaagaccacc ggctggcggg gcggccacgt ggtggagggc
      181 ctggccggcg agctggagca gctgcgggcc aggctggagc accaccctca gggccagcgg
      241 gagccctccg gcggctgcaa gctgggcctg ggtaccgaga acctgtactt ccaatccatg
      301 tttgaaatta agaagatctg ttgcattggt gcaggctatg ttggaggacc cacatgtagt
      361 gtcattgctc atatgtgtcc tgaaatcagg gtaacggttg ttgatgtcaa tgaatcaaga
      421 atcaatgcgt ggaattctcc tacacttcct atttatgagc caggactaaa agaagtggta
      481 gaatcctgtc gaggaaaaaa tctttttttt tctaccaata ttgatgatgc catcaaagaa
      541 gctgatcttg tatttatttc tgtgaatact ccaacaaaaa cctatggaat ggggaaaggc
      601 cgggcagcag atctgaagta tattgaagct tgtgctagac gcattgtgca aaactcaaat
      661 gggtacaaaa ttgtgactga gaaaagcgca gttccggtgc gggcagcaga aagtatccgt
      721 cgcatatttg atgcaaacac aaaacccaac ttgaatttac aggtgctgtc caaccctgag
      781 tttctggcag agggaacagc catcaaggac ctaaagaacc cagacagagt actgattgga
      841 ggggatgaaa ctccagaggg ccagagagct gtgcaggccc tgtgtgctgt atatgagcac
      901 tgggttccca gagaaaagat cctcaccact aatacttggt cttcagagct ttccaaactg
      961 gcagcaaatg cttttcttgc ccagagaata agcagcatta actccataag tgctctgtgt
     1021 gaagcaacag gagctgatgt agaagaggta gcaacagcga ttggaatgga ccagagaatt
     1081 ggaaacaagt ttctaaaagc cagtgttggg tttggtggga gctgtttcca aaaggatgtt
     1141 ctgaatttgg tttatctctg tgaggctctg aatttgccag aagtagctcg ttattggcag
     1201 caggtcatag acatgaatga ctaccagagg aggaggtttg cttcccggat catagatagt
     1261 ctgtttaata cagtaactga taagaagata gctattttgg gatttgcatt caaaaaggac
     1321 actggtgata caagagaatc ttctagtata tatattagca aatatttgat ggatgaaggt
     1381 gcacatctac atatatatga tccaaaagta cctagggaac aaatagttgt ggatctttct
     1441 catccaggtg tttcagagga tgaccaagtg tcccggctcg tgaccatttc caaggatcca
     1501 tatgaagcat gtgatggtgc ccatgctgtt gttatttgca ctgagtggga catgtttaag
     1561 gaattggatt atgaacgcat tcataaaaaa atgctaaagc cagcctttat cttcgatgga
     1621 cggcgtgtcc tggatgggct ccacaatgaa ctacaaacca ttggcttcca gattgaaaca
     1681 attggcaaaa aggtgtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
     1741 agcttgcggc cgcactcgag caccaccacc accaccactg a
//
