LOCUS       sequence-490865-        3255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern 
            in the presence of ZFa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: (loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(116..118,119..121,122..835)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(161..182)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    847..1071
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1182..1201)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1193..2002
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1260..1279)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2092..2680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2581..2600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2845..2931
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3023..3050
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3058..3081
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    complement(join(3249..3255,1..27))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgtatagcat acattatacg aagttatcgg atttgatctg atcaagcaac tctagagggt
       61 atataatggg ggccactagt ctactaccag agctcatcgc tagcaatgag ccaccatggt
      121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      181 cgtaaacggc cacaagttca gcgtccgcgg cgagggcgag ggcgatgcca ccaacggcaa
      241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      301 gaccacctta ggctacggcg tggcctgctt cgcccgctac cccgaccaca tgaagcagca
      361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tctctttcaa
      421 ggacgacggt acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      481 ccgcatcgtg ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      541 ggagtacaac ttcaacagcc acaaggtcta tatcacggcc gacaagcaga agaacggcat
      601 caaggctaac ttcaagaccc gccacaacgt tgaggacggc ggcgtgcagc tcgccgacca
      661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      721 gagccatcag tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgaa
      781 ggagagggtg accgccgccg ggattacaca tgacatggac gagctgtaca agtaacaacg
      841 ttgcacctgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      901 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      961 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     1021 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcgctcgctg
     1081 actgcaggtg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1141 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     1201 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1261 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     1321 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1381 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1441 gcattttatc cgtactcctg gtgatgcatg gttactcacc actgcgatcc ccggaaaaac
     1501 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1561 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1621 cgtatttcgt ctagctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     1681 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     1741 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     1801 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     1861 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     1921 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1981 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacatt attcagattg
     2041 ggccccgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2761 tgattctgtg gataaccgta ttaccgctag catggatctc ggggacgtct aactactaag
     2821 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga agactgggcc
     2881 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     2941 gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     3001 actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3061 caaactcttc ctgttagtta gttacttaag ctcgggccca cctgcagtct acttactcgg
     3121 atcgggagat ctgaattgga gtgaggacgt gtagagtgag gacgtgcgga gtgaggacgt
     3181 gtagagtgag gacgtgcgga gtgaggacgt gtagagtgag gacgtgccct cctctggatc
     3241 aaatccgaat aactt
//
