LOCUS       sequence-49087-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD3
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             727..747
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             748..765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             766..789
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcgactgc tgacgtggga tgtgaaggac acgctgctca ggctccgcca ccccttaggg
       61 gaggcctatg ccaccaaggc ccgggcccat gggctggagg tggagccctc agccctggaa
      121 caaggcttca ggcaggcata cagggctcag agccacagct tccccaacta cggcctgagc
      181 cacggcctaa cctcccgcca gtggtggctg gatgtggtcc tgcagacctt ccacctggcg
      241 ggtgtccagg atgctcaggc tgtagccccc atcgctgaac agctttataa agacttcagc
      301 cacccctgca cctggcaggt gttggatggg gctgaggaca ccctgaggga gtgccgcaca
      361 cggggtctga gactggcagt gatctccaac tttgaccgac ggctagaggg catcctgggg
      421 ggccttggcc tgcgtgaaca cttcgacttt gtgctgacct ccgaggctgc tggctggccc
      481 aagccggacc cccgcatttt ccaggaggcc ttgcggcttg ctcatatgga accagtagtg
      541 gcagcccatg ttggggataa ttacctctgc gattaccagg ggcctcgggc tgtgggcatg
      601 cacagcttcc tggtggttgg cccacaggca ctggaccccg tggtcaggga ttctgtacct
      661 aaagaacaca tcctcccctc tctggcccat ctcctgcctg cccttgactg cctagagggc
      721 tcagcagaga acctctactt ccaatcgcac catcatcacc accatgatta caaggatgac
      781 gacgataagt gaggatcc
//
