LOCUS       sequence-490889-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for ARIH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag ARIH2 binding E2 variant L3A2-4
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..30
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             499..543
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(719..741)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(886..904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaatcggatg aaaacctgta ttttcaggga tccatgcggg cggccctgct gaggctgccg
       61 aaggagcttg aagaaatccg caaagcaggg atgaaaaact tccgtaacat ccaggttgat
      121 gaagctaatt tattgacttg gcaagggctt attgttcctg acaaccctcc atatgataag
      181 ggagccttca gaatcgaaat caactttcca gcagagtacc cattcaaacc accgaagatc
      241 acatttaaaa caaagatcta tcacccaaac atcgacgaaa aggggcaggt ctgtctgcca
      301 gtaattagtg ccgaaaactg gaagccagca accaaaaccg accaagtaat ccagtccctc
      361 atagcactgg tgaatgaccc ccagcctgag cacccgcttc gggctgacct agctgaagaa
      421 tactctaagg accgtaaaaa attcgcaaag aatgctgaag agtttacaaa gaaatatggg
      481 gaaaagcgac ctgtggacgg tcttaacgac atcttcgagg ctcaaaagat tgaatggcat
      541 gaataagcgg ccgcatcggg ctgacctagc tgaagaatac tctaaggacc gtaaaaaatt
      601 cgcaaagaat gctgaagagt ttacaaagaa atatggggaa aagcgacctg tggactaagc
      661 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
      721 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
      781 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
      841 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc
      901 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      961 ggggaaatgt gcgcggaacc ccta
//
