LOCUS       sequence-490895-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for ANKIB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag ANKIB1 binding E2 variant L3AN-2
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..37
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(566..588)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(733..751)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        820..922
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctccaaaa tcggatgaaa acctgtattt tcagggatcc tgggcggcct cgaagaggct
       61 gaggtgggag cttgaagaaa tccgcaaagc agggatgaaa aacttccgta acatccaggt
      121 tgatgaagct aatttattga cttggcaagg gcttattgtt cctgacaacc ctccatatga
      181 taagggagcc ttcagaatcg aaatcaactt tccagcagag tacccattca aaccaccgaa
      241 gatcacattt aaaacaaaga tctatcaccc aaacatcgac gaaaaggggc aggtctgtct
      301 gccagtaatt agtgccgaaa actggaagcc agcaaccaaa accgaccaag taatccagtc
      361 cctcatagca ctggtgaatg acccccagcc tgagcacccg cttcgggctg acctagctga
      421 agaatactct aaggaccgta aaaaattcgc aaagaatgct gaagagttta caaagaaata
      481 tggggaaaag cgacctgtgg actaagcggc cgcatcgtga ctgactgacg atctgcctcg
      541 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      601 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      661 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt
      721 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      781 tttcttagac gtcaggtggc acttttcggg ggaaatgtgc gcggaacccc tatttgttta
      841 tttttctaat acattcaaat atgtatccgc tcatgagaca ataaccctga taatgcttca
      901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttatcccttt
      961 tttg
//
