LOCUS       sequence-490896-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for ARIH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag ARIH1 binding E2 variant L3A1-2
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             508..552
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(728..750)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(895..913)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        981..1085
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatgcctgc ggccagtcat
       61 aggctgaaga aggagcttga agaaatccgc aaagcaggga tgaaaaactt ccgtaacatc
      121 caggttgatg aagctaattt attgacttgg caagggctta ttgttcctga caaccctcca
      181 tatgataagg gagccttcag aatcgaaatc aactttccag cagagtaccc attcaaacca
      241 ccgaagatca catttaaaac aaagatctat cacccaaaca tcgacgaaaa ggggcaggtc
      301 tgtctgccag taattagtgc cgaaaactgg aagccagcaa ccaaaaccga ccaagtaatc
      361 cagtccctca tagcactggt gaatgacccc cagcctgagc acccgcttcg ggctgaccta
      421 gctgaagaat actctaagga ccgtaaaaaa ttcgcaaaga atgctgaaga gtttacaaag
      481 aaatatgggg aaaagcgacc tgtggacggt cttaacgaca tcttcgaggc tcaaaagatt
      541 gaatggcatg aataagcggc cgcatcgggc tgacctagct gaagaatact ctaaggaccg
      601 taaaaaattc gcaaagaatg ctgaagagtt tacaaagaaa tatggggaaa agcgacctgt
      661 ggactaagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      721 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      781 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      841 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
      901 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tggaaaagga
     1081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gsattttgcc
     1141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa gatgctgaag atcagttggg
     1201 gggccc
//
