LOCUS       sequence-490897-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for HOIL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag HOIL1 binding E2 variant L3HL-1
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             505..549
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(725..747)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(892..910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        978..1082
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatggcggc cagcaggagg
       61 ctgatgaagg agcttgaaga aatccgcaaa gcagggatga aaaacttccg taacatccag
      121 gttgatgaag ctaatttatt gacttggcaa gggcttattg ttcctgacaa ccctccatat
      181 gataagggag ccttcagaat cgaaatcaac tttccacagc agtacccaat tccgccaccg
      241 aagatcacat ttaaaacaaa gatctatcac ccaaacatcg acgaaaaggg gcaggtctgt
      301 ctgccagtaa ttagtgccga aaactggaag ccagcaacca aaaccgacca agtaatccag
      361 tccctcatag cactggtgaa tgacccccag cctgagcacc cgcttcgggc tgacctagct
      421 gaagaatact ctaaggaccg taaaaaattc gcaaagaatg ctgaagagtt tacaaagaaa
      481 tatggggaaa agcgacctgt ggacggtctt aacgacatct tcgaggctca aaagattgaa
      541 tggcatgaat aagcggccgc atcgggctga cctagctgaa gaatactcta aggaccgtaa
      601 aaaattcgca aagaatgctg aagagtttac aaagaaatat ggggaaaagc gacctgtgga
      661 ctaagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
      721 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      781 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
      841 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga
      901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1141 ctgtttttgc tcacccaaaa accctgg
//
