LOCUS       sequence-490899-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for LUBAC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag LUBAC binding E2 variant L3LU-2
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(569..591)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(736..754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        822..926
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccatcctcca aaatcggatg aaaacctgta ttttcaggga tccatggcgg ccagcaggag
       61 gctgatgaag gagcttgaag aaatccgcaa agcagggatg aaaaacttcc gtaacatcca
      121 ggttgatgaa gctaatttat tgacttggca agggcttatt gttcctgaca accctccata
      181 tgataaggga gccttcagaa tcgaaatcaa ctttccagca gagtacccat tcaaaccacc
      241 gaagatcaca tttaaaacaa agatctatca cccaaacatc gacgaaaagg ggcaggtctg
      301 tctgccagta attagtgccg aaaactggat ggcgccgacc aaaaccgacc aagtaatcca
      361 gtccctcata gcactggtga atgaccccca gcctgagcac ccgcttcggg ctgacctagc
      421 tgaagaatac tctaaggacc gtaaaaaatt cgcaaagaat gctgaagagt ttacaaagaa
      481 atatggggaa aagcgacctg tggactaagc ggccgcatcg tgactgactg acgatctgcc
      541 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
      601 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      661 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt
      721 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      781 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      961 ccttgytt
//
