LOCUS       sequence-49090-e        1544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1544)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PHF8
FEATURES             Location/Qualifiers
     source          1..1544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..357
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             364..384
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      1335..1344
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt atgagcgata aaattattca cctgactgac
       61 gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga tttctgggca
      121 gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc tgacgaatat
      181 cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
      241 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc
      301 aaagtgggcg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggccggt
      361 accgagaact tgtacttcca atccatgcca gtgaagaccg ggagccctac gttcgtcaga
      421 gagctccgga gtaggacttt tgacagctca gatgaagtga ttctgaagcc cactggaaat
      481 caactgaccg tggaattcct ggaagaaaat agcttcagtg tgcccatcct ggtcctgaag
      541 aaggatgggt tgggcatgac gctgccctcg ccatcattca ctgtgaggga tgttgaacac
      601 tatgttggtt ctgacaaaga gattgatgtg attgatgtga cccgccaggc tgactgcaag
      661 atgaagcttg gtgattttgt gaaatactat tacagcggga agagggagaa agtcctcaat
      721 gtcattagtt tggaattctc tgataccaga ctttctaacc ttgtggagac accgaagatt
      781 gttcgaaagc tgtcatgggt cgaaaacttg tggccagagg aatgtgtctt tgagagaccc
      841 aatgtacaga agtactgcct catgagtgtg cgagatagct atacagactt tcacattgac
      901 tttggtggca cctctgtctg gtaccatgta ctcaagggtg aaaagatctt ctacctgatc
      961 cgcccaacaa atgccaatct gactctcttt gagtgctgga gcagttcctc taatcagaat
     1021 gagatgttct ttggggacca ggtggacaag tgctacaagt gttccgtgaa gcaaggacag
     1081 acacttttca ttcccacagg gtggatccat gctgtgctga cgcctgtgga ctgccttgcc
     1141 tttggaggga acttcttaca cagccttaac atcgagatgc agctcaaagc ctatgagatt
     1201 gagaagcggc tgagcacagc agacctcttc agattcccca actttgagac catctgttgg
     1261 tatgtgggaa agcacatcct ggacatcttt cgcggtttgc gagagaacag gagacaccct
     1321 gcctcctacc tggtccatgg tggcaaagcc ttgaacttgg cctttagagc ctggacaagg
     1381 aaagaagctc tgccagacca tgaggatgag atcccggaga cagtgcgaac cgtacagctc
     1441 attaaagatc tggccaggga gatccgcctg gtggaagaca tcttccaaca gaactgacag
     1501 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gctt
//
