LOCUS       sequence-490900-        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for LUBAC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag LUBAC binding E2 variant D3LU-1
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(550..572)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(717..735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        803..907
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatggcact gaaacgcatt
       61 aacaaagaac tgagcgatct ggcacgtgat ccgcctgcac agattagcgc aggtccggtt
      121 ggtgatgata tgtttcattg gcaggcaacc attatgggtc cgaatgatag cccgtatcaa
      181 ggtggtgttt tttttctgac cattcacttt ccgaccgact atccgtttaa accgcctaaa
      241 gttgcattta ccacgcgtat ttatcatccg aacattaata gcaatggcag catttgtctg
      301 gatattctgc gttggtcttg gctgaacgca ctgaccatta gcaaagttct gctgagcatt
      361 gcaagcctgc tggatgatcc gaatccggat gatcctctgg ttccggaaat tgcacgtatc
      421 tataaaaccg atcgcgataa atacaatcgc attagccgtg aatggaccca gaaatatgca
      481 atgtaatgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
      541 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      601 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
      661 agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct
      721 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1081 tcgccccgaa gaacgttttc catgatgagc mcttttaaag ttctgctatg tggcgcggta
//
