LOCUS       sequence-490905-       14705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for PaqCI Golden Gate assembly of ISAam1 guides into 
            ISAam1 plant expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14705)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 14705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pMK024
FEATURES             Location/Qualifiers
     source          1..14705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             438..1067
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1496..2569
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2635..2829
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3065..3087)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3173..3313
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3228..3247
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3328..3345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3499..4087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3579..3598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4174..4968)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5393..5417
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5495..5669
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5710..6735)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6802..7478)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6845..6865)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7669..7690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7705..7710,7711..7728,7729..7735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7743..7759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7748..7770
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7767..7783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7767..7783
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     ncRNA           complement(8434..8501)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    8513..8637
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8678..8983)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8780..8799)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9303..9983)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFATVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        complement(join(10037..10043,10044..10061,10062..10067))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10092..10216)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(11478..11498)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11505..11552)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     primer_bind     complement(13599..13616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13599..13615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13608..13630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    13818..13842
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
       61 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
      121 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
      181 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
      241 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
      301 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
      361 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
      421 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
      481 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
      541 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
      601 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
      661 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
      721 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
      781 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
      841 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
      901 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
      961 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     1021 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     1081 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     1141 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     1201 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     1261 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     1321 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     1381 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     1441 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     1501 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     1561 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     1621 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     1681 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     1741 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     1801 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     1861 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     1921 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     1981 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2041 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2101 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2161 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     2221 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     2281 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     2341 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     2401 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     2461 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     2521 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     2581 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     2641 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     2701 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     2761 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     2821 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     2881 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     2941 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3001 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3061 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3181 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     3241 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3301 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3901 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4141 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     4201 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     4261 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     4321 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     4381 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     4441 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     4501 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     4561 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     4621 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     4681 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     4741 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     4801 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     4861 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     4921 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     4981 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     5041 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     5101 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     5161 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     5221 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     5281 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     5341 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     5401 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     5461 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     5521 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     5581 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     5641 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag atcccggtcg
     5701 gcatctactc tatttctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc
     5761 gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg
     5821 cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc
     5881 atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat
     5941 atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt
     6001 ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg
     6061 ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt
     6121 caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc
     6181 ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat
     6241 cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt
     6301 agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc
     6361 ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg gcagttcggt
     6421 ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg gagatgcaat aggtcaggct
     6481 ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg
     6541 ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata
     6601 tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat
     6661 caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag
     6721 ttcaggcttt ttcatatctc attgcccccc ggatctgcga aagctcgaga gagatagatt
     6781 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
     6841 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     6901 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     6961 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     7021 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     7081 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     7141 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     7201 gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg
     7261 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     7321 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     7381 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     7441 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     7501 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
     7561 gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7741 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcgt
     7801 tgtcaatcaa ttggcaagtc ataaaatgca ttaaaaaata ttttcatact caactacaaa
     7861 tccatgagta taactataat tataaagcaa tgattagaat ctgacaagga ttctggaaaa
     7921 ttacataaag gaaagttcat aaatgtctaa aacacaagag gacatacttg tattcagtaa
     7981 catttgcagc ttttctaggt ctgaaaatat atttgttgcc tagtgaataa gcataatggt
     8041 acaactacaa gtgttttact cctcatatta acttcggtca ttagaggcca cgatttgaca
     8101 catttttact caaaacaaaa tgtttgcata tctcttataa tttcaaattc aacacacaac
     8161 aaataagaga aaaaacaaat aatattaatt tgagaatgaa caaaaggacc atatcattca
     8221 ttaactcttc tccatccatt tccatttcac agttcgatag cgaaaaccga ataaaaaaca
     8281 cagtaaatta caagcacaac aaatggtaca agaaaaacag ttttcccaat gccataatac
     8341 tcaaactcag taggattctg gtgtgtgcgc aatgaaactg atgcattgaa cttgacgaac
     8401 gttgtcgaaa ccgatgatac gaacgaaagc tctgtcccat tcgccatgcc gaagcatgtt
     8461 gcccagccgg cgccagcgag gaggctggga ccatgccggc ccatcgcagg tgaccacttt
     8521 gtacaagaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     8581 tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt cactatggtc
     8641 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
     8701 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
     8761 cggaggccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
     8821 gcaccaccgg gtaaagttca cgggggactt tatctgacag cagacgtgca ctggccaggg
     8881 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
     8941 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
     9001 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
     9061 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
     9121 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
     9181 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
     9241 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     9301 ttttagtaag ccggatcctc tagattacgc cccgcctgcc actcatcgca gtactgttgt
     9361 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     9421 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacggtg
     9481 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     9541 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     9601 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     9661 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     9721 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     9781 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     9841 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagcg
     9901 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     9961 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgacggatcc
    10021 taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggttcct
    10081 aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
    10141 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
    10201 ttttgtacaa acttgtcacc tgcttttgtg acatgctccc accacctacg cttcgcttag
    10261 aggtgggggc ttctcaggta atcccatctc atgacgggaa gttgactgag cgatccccgt
    10321 gtgtcccacg gttcgaggac aagttaggct attgctaatc gtttcatgcc ctcatgtttg
    10381 atattgattg ccgcattgac gtccctgtcg ctctcgaatc cacattcaca gcggaatgta
    10441 cgctcagaaa gcgataaaga ttcctttact tgatcgcaac atgaacaagt tttagatgac
    10501 ggaaaccact tatctatttt gataagcttt ttcccttgct cctctaactt gtattggaga
    10561 aatgtcgtga acatccccca gccgttgtca tgaacgctct tgccgaaatt caggacttgc
    10621 gacatccctt tcatgttgag gttttcgatg accacgcaat catactgttt cgctaattcg
    10681 tatgattttt tgtgtagaaa gtcttttcgt tggttcgcta ttttctcatg tagcttcgct
    10741 actttcaaac gctgtttgtg ccaacgatta gaacccttct ttcggcgtga taatatccgt
    10801 tgttctttcg ctaatttttc caatgcttgc cgatagaaac gaggataatt ggctctctta
    10861 ccctcgctat cgacaaataa accattcatg gaaaaatcaa gcccaacaac cgtttgtatt
    10921 tctttgggga caggttgatg ttcgtattcg gtgagaatag aaacatagta ttttcctgtt
    10981 tttgttttct tgatcgtaca agccttgatg atatggtggg caggaatctc ccgatgttgc
    11041 ttgaacttga cccatttcag tttgggtaac ttgatatagc catctgaaag ttgaatattg
    11101 ccattgacca catttgtggt gtacgactgt ttcgccttgc ggtttttgaa ctttggaaat
    11161 ccagcacgac ccgaaaagaa attttgaaac gctttctgca aattcaattg ggcgtttgct
    11221 aacgcaaggc tatccacttc tttaagccaa ggaaactcct ttttgtactt ggcaggggtc
    11281 ggaaatgttt gttttttcaa agcttctttg tcgtctttga acttttcata aatttgtatg
    11341 cgttcttcaa gcattttgtt ataaacgaaa cggacacaac cgaaggtttt ggcaagcagc
    11401 tgttcttgtt cttttgttgg gtacagacgg aatttatatg atttgtttac catagcagct
    11461 ggaactccgt ggattccaac ctttctcttc ttctttggag ccatcttatc atcatcatcc
    11521 ttgtaatcct tatcatcatc atccttgtaa tccatctgtt aatcagaaaa actcagatta
    11581 atcgacaaat tcgatcgcac aaactagaaa ctaacaccag atctagatag aaatcacaaa
    11641 tcgaagagta attattcgac aaaactcaaa ttatttgaac aaatcggatg atatctatga
    11701 aaccctaatc gagaattaag atgatatcta acgatcaaac ccagaaaatc gtcttcgatc
    11761 taagattaac agaatctaaa ccaaagaaca tatacgaaat tgggatcgaa cgaaaacaaa
    11821 atcgaagatt ttgagagaat aaggaacaca gaaatttacc ttgatcacgg tagagagaat
    11881 tgagagaaag tttttaagat tttgagaaat tgaaatctga attgtgaaga agaagagctc
    11941 tttgggtatt gttttataga agaagaagaa gaaaagacga ggacgactag gtcacgagaa
    12001 agctaaggcg gtgaagcaat agctaataat aaaatgacac gtgtattgag cgttgtttac
    12061 acgcaaagtt gtttttggct aattgcctta tttttaggtt gaggaaaagt atttgtgctt
    12121 tgagttgata aacacgactc gtgtgtgccg gctgcaacca ctttgacgcc gtttattact
    12181 gactcgtcga caaccacaat ttctaacggt cgtcataaga tccagccgtt gagatttaac
    12241 gatcgttacg atttatattt ttttagcatt atcgttttat tttttaaata tacggtggag
    12301 ctgaaaattg gcaataattg aaccgtgggt cccactgcat tgaagcgtat ttcgtatttt
    12361 ctagaattct tcgtgcttta tttcttttcc tttttgtttt tttttgccat ttatctaatg
    12421 caagtgggct tataaaatca gtgaatttct tggaaaagta acttctttat cgtataacat
    12481 attgtgaaat tatccatttc ttttaatttt ttagtgttat tggatatttt tgtatgatta
    12541 ttgatttgca taggataatg acttttgtat caagttggtg aacaagtctc gttaaaaaag
    12601 gcaagtggtt tggtgactcg atttattctt gttatttaat tcatatatca atggatctta
    12661 tttggggcct ggtccatatt taacactcgt gttcagtcca atgaccaata atattttttc
    12721 attaataaca atgtaacaag aatgatacac aaaacattct ttgaataagt tcgctatgaa
    12781 gaagggaact tatccggtcc tagatcatca gttcatacaa acctccatag agttcaacat
    12841 cttaaacaag aatatcctga tctgaagaat gtggaggctt tagtcccttg gatacttggg
    12901 aggctgtgga agaacagaaa cgagctggtg cttaaaggga gggaatttgg aaccaatgag
    12961 gtattagtaa ggacacaaga agatgcagat gagtggatta gaaggaaaga ggctcagaat
    13021 gtaaggaaag caccaaccac aacaccgtcg ggacagacga gaaacatggg aagctccacc
    13081 acaatcttgg gttaagtgca actttgatgg ggcatggcca acagaaggat taaaatgtgg
    13141 cttagggtgg gtgcttcgcg atcatacagg gaaggtgtta tggttaggtg caagagctgt
    13201 ggtaaaagta agaagcgtgc tggaagtaga agtggaggct cttagatggg ctgtgctgtc
    13261 attatcccga ttcaattata ggaagatcat ttttgaggtg gattctcagc aacttgtgtc
    13321 tttggttaca tgaaagttat gcttgtcaag tctcaatcca attatccaag acataaagta
    13381 tctacttagc aagtttgagg attttatgct tgtgcataca agccgagaag gaaatggagt
    13441 ggcagataga atagctaagg aatctctttc ttttgagaat tatgatctaa agttgtattc
    13501 tattgtacca atttgggtta aaagctctgt tgagctagac tggtaccttc ctaggatact
    13561 agtgatctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
    13621 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    13681 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    13741 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
    13801 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
    13861 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
    13921 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
    13981 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
    14041 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
    14101 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
    14161 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
    14221 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
    14281 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
    14341 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
    14401 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
    14461 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
    14521 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
    14581 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
    14641 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
    14701 tcgac
//
