LOCUS       sequence-49091-e        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MMAA
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga aggaccacac agaaggactt tctgataaag agcaaagatt tgtggataaa
      121 ctttatactg gtttaatcca agggcaaagg gcctgtttag cagaggccat aactcttgta
      181 gaatcaactc acagcaggaa aaaggagtta gcccaggtgc ttcttcagaa agtattactt
      241 taccacagag aacaagaaca atcaaataaa ggaaaaccac tagcatttcg agtaggattg
      301 tctgggcccc ctggtgctgg aaaatcaaca tttatagaat attttggaaa aatgcttact
      361 gagagagggc acaaattatc tgtgctagct gtggaccctt cttcttgtac tagtggtgga
      421 tcactcttag gtgataaaac ccgaatgact gagttatcaa gagatatgaa tgcatacatc
      481 aggccatctc ctactagagg aactttagga ggcgtgacaa ggaccacaaa tgaagctatt
      541 ctgttgtgtg aaggagcggg atatgacata attcttattg aaaccgttgg tgtgggtcag
      601 tcggagtttg ctgttgctga catggttgac atgtttgttt tactactgcc accagcagga
      661 ggagatgagc tgcagggtat caaaaggggt ataatcgaga tggcagatct ggtagctgta
      721 actaaatctg atggagactt gattgtgcca gctcgaagga tacaagcgga atatgtgagt
      781 gcactgaaat tactccgcaa acgttcacaa gtctggaaac caaaggtaat tcgtatttct
      841 gcccgaagtg gagaggggat ctctgaaatg tgggataaaa tgaaagattt ccaggaccta
      901 atgcttgcca gtggggagct gactgccaaa cgacggaagc aacagaaagt ttggatgtgg
      961 aatctcattc aggaaagtgt gttagagcat ttcaggaccc accccacagt ccgggaacag
     1021 attccacttc tggaacaaaa ggttctcatt ggggccctgt ccccaggact agcagcagac
     1081 ttcttgttaa aagcttttaa aagcagagac taacagtaaa ggtggatacg gatccgaa
//
