LOCUS       sequence-490929-        8036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pXeDNA3.1-HsTRPV1 encodes human TRPV1 in CMV/Xenopus beta‑globin UTR
            scaffold for robust expression in mammalian cells and Xenopus 
            oocytes; BGH polyA, AmpR, NeoR, lacZ-alpha for blue‑white 
            screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Guan, Li
  TITLE     Development of a Dual Expression Plasmid for Xenopus and Mammalian 
            Systems
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pXeDNA3.1-HsTRPV1
FEATURES             Location/Qualifiers
     source          1..8036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        823..841
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           849..891
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(3430..3448)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           3449..3574
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3627..3644)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3633..3857
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3903..4331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3990..4009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4200..4221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4340..4360)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4345..4674
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4525..4660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4587..4606
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4741..5535
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4795..4814)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5405..5424
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5709..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5746..5765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5800..5819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5879..5895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5879..5895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5892..5914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5903..5919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5927..5933,5934..5951,5952..5957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5972..5993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6110..6127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6281..6869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6361..6380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7040..7831,7832..7900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7663..7682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7901..8005)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct cataatacga ctcactatag
      841 gcagcttgct tgttcttttt gcagaagctc agaataaacg ctcaactttg gccagcggac
      901 catgaagaaa tggagcagca cagacttggg ggcagctgcg gacccactcc aaaaggacac
      961 ctgcccagac cccctggatg gagaccctaa ctccaggcca cctccagcca agccccagct
     1021 ctccacggcc aagagccgca cccggctctt tgggaagggt gactcggagg aggctttccc
     1081 ggtggattgc cctcacgagg aaggtgagct ggactcctgc ccgaccatca cagtcagccc
     1141 tgttatcacc atccagaggc caggagacgg ccccaccggt gccaggctgc tgtcccagga
     1201 ctctgtcgcc gccagcaccg agaagaccct caggctctat gatcgcagga gtatctttga
     1261 agccgttgct cagaataact gccaggatct ggagagcctg ctgctcttcc tgcagaagag
     1321 caagaagcac ctcacagaca acgagttcaa agaccctgag acagggaaga cctgtctgct
     1381 gaaagccatg ctcaacctgc acgacggaca gaacaccacc atccccctgc tcctggagat
     1441 cgcgcggcaa acggacagcc tgaaggagct tgtcaacgcc agctacacgg acagctacta
     1501 caagggccag acagcactgc acatcgccat cgagagacgc aacatggccc tggtgaccct
     1561 cctggtggag aacggagcag acgtccaggc tgcggcccat ggggacttct ttaagaaaac
     1621 caaagggcgg cctggattct acttcggtga actgcccctg tccctggccg cgtgcaccaa
     1681 ccagctgggc atcgtgaagt tcctgctgca gaactcctgg cagacggccg acatcagcgc
     1741 cagggactcg gtgggcaaca cggtgctgca cgccctggtg gaggtggccg acaacacggc
     1801 cgacaacacg aagtttgtga cgagcatgta caatgagatt ctgatgctgg gggccaaact
     1861 gcacccgacg ctgaagctgg aggaactcac caacaagaag ggaatgacgc cgctggctct
     1921 ggcagctggg accgggaaga tcggggtctt ggcctatatt ctccagcggg agatccagga
     1981 gcccgagtgc aggcacctgt ccaggaagtt caccgagtgg gcctacgggc ccgtgcactc
     2041 ctcgctgtac gacctgtcct gcatcgacac ctgcgagaag aactcggtgc tggaggtgat
     2101 cgcctacagc agcagcgaga cccctaatcg ccacgacatg ctcttggtgg agccgctgaa
     2161 ccgactcctg caggacaagt gggacagatt cgtcaagcgc atcttctact tcaacttcct
     2221 ggtctactgc ctgtacatga tcatcttcac catggctgcc tactacaggc ccgtggatgg
     2281 cttgcctccc tttaagatgg aaaaaactgg agactatttc cgagttactg gagagatcct
     2341 gtctgtgtta ggaggagtct acttcttttt ccgagggatt cagtatttcc tgcagaggcg
     2401 gccgtcgatg aagaccctgt ttgtggacag ctacagtgag atgcttttct ttctgcagtc
     2461 actgttcatg ctggccaccg tggtgctgta cttcagccac ctcaaggagt atgtggcttc
     2521 catggtattc tccctggcct tgggctggac caacatgctc tactacaccc gcggtttcca
     2581 gcagatgggc atctatgccg tcatgataga gaagatgatc ctgagagacc tgtgccgttt
     2641 catgtttgtc tacatcgtct tcttgttcgg gttttccaca gcggtggtga cgctgattga
     2701 agacgggaag aatgactccc tgccgtctga gtccacgtcg cacaggtggc gggggcctgc
     2761 ctgcaggccc cccgatagct cctacaacag cctgtactcc acctgcctgg agctgttcaa
     2821 gttcaccatc ggcatgggcg acctggagtt cactgagaac tatgacttca aggctgtctt
     2881 catcatcctg ctgctggcct atgtaattct cacctacatc ctcctgctca acatgctcat
     2941 cgccctcatg ggtgagactg tcaacaagat cgcacaggag agcaagaaca tctggaagct
     3001 gcagagagcc atcaccatcc tggacacgga gaagagcttc cttaagtgca tgaggaaggc
     3061 cttccgctca ggcaagctgc tgcaggtggg gtacacacct gatggcaagg acgactaccg
     3121 gtggtgcttc agggtggacg aggtgaactg gaccacctgg aacaccaacg tgggcatcat
     3181 caacgaagac ccgggcaact gtgagggcgt caagcgcacc ctgagcttct ccctgcggtc
     3241 aagcagagtt tcaggcagac actggaagaa ctttgccctg gtcccccttt taagagaggc
     3301 aagtgctcga gataggcagt ctgctcagcc cgaggaagtt tatctgcgac agttttcagg
     3361 gtctctgaag ccagaggacg ctgaggtctt caagagtcct gccgcttccg gggagaagtg
     3421 accgctggtc ctttagtgag ggttaattaa ccagcctcaa gaacacccga atggagtctc
     3481 taagctacat aataccaact tacactttac aaaatgttgt cccccaaaat gtagccattc
     3541 gtatctgctc ctaataaaaa gaaagtttct tcacattcta aaaaaaaaaa aaaaaaccgg
     3601 ggggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3661 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3721 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3781 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3841 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     3901 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3961 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4021 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4081 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4141 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4201 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4261 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4321 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4381 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4501 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4561 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4621 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4681 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     4741 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4801 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4861 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     4921 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     4981 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5041 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5101 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5161 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5221 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5281 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5341 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5401 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5461 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5521 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5581 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5641 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5701 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5821 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6061 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
