LOCUS       sequence-49093-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GUCY1B3
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             643..663
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             664..681
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcacaagc gtccggttcc ggccaaacgc tatgacaacg tgactattct gttctccggt
       61 atcgtagggt ttaatgcatt ttgtagcaaa catgcgtcgg gcgagggtgc tatgaagatt
      121 gttaacctgc tgaatgatct gtacacacgt ttcgatacgc tgaccgactc tcgcaaaaac
      181 ccgtttgtgt ataaagtcga aactgttggc gataagtata tgacagtgag tggtctgccg
      241 gagccgtgca tccatcacgc ccgttcaatt tgtcatctgg cactggacat gatggaaatt
      301 gcggggcagg tacaggtcga tggcgaaagc gttcaaatca cgattggtat ccacaccggg
      361 gaggtggtaa ctggcgtcat tggtcagcgc atgccgcgtt actgcctgtt cggcaatacg
      421 gttaacctga cctcccgcac agaaaccact ggtgaaaaag ggaaaatcaa tgtgtcggag
      481 tatacatacc gttgtctgat gtctccggaa aacagtgacc cgcaatttca tctggagcac
      541 cgcggcccgg taagcatgaa gggtaaaaaa gaaccgatgc aggtctggtt cctgtcacgt
      601 aagaatacgg gcaccgaaga gactaaacag gatgatgacg cagagaacct ctacttccaa
      661 tcgcaccatc atcaccacca tgattacaag gatgacgacg ataagtgagg atcc
//
