LOCUS       sequence-49095-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GMFG
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg tgtgcgaggt agacccagag ctaacagaaa agctgaggaa attccgcttc
      121 cgaaaagaga cagacaatgc agccatcata atgaaggtgg acaaagaccg gcagatggtg
      181 gtgctggagg aagaatttca gaacatttcc ccagaggagc tcaaaatgga gttgccggag
      241 agacagccca ggttcgtggt ttacagctac aagtacgtgc atgacgatgg ccgagtgtcc
      301 taccctttgt gtttcatctt ctccagccct gtgggctgca agccggaaca acagatgatg
      361 tatgcaggga gtaaaaacag gctggtgcag acagcagagc tcacaaaggt gttcgaaatc
      421 cgcaccactg atgacctcac tgaggcctgg ctccaagaaa agttgtcttt ctgacagtaa
      481 aggtggatac ggatccgaa
//
