LOCUS       sequence-49098-e        1509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1509)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DNPEP
FEATURES             Location/Qualifiers
     source          1..1509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1438..1458
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1459..1476
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1477..1500
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgagcggac acagccccac gcgcggggcc atgcaggtgg ccatgaacgg taaggcccgc
       61 aaagaggcgg tgcagactgc ggctaaggaa ctcctcaagt tcgtgaaccg gagtccctct
      121 cctttccatg ctgtggctga atgccgcaac cgccttctcc aggctggctt cagtgaactc
      181 aaggagactg agaaatggaa tattaagccc gagagcaagt acttcatgac caggaactcc
      241 tccaccatca tagcttttgc tgtagggggc cagtacgttc ctggcaatgg cttcagcctc
      301 atcggggccc acacggacag cccctgcctc cgggtgaaac gtcggtctcg ccgcagccag
      361 gtgggcttcc agcaagtcgg tgtggagacc tatggtggtg ggatctggag cacctggttt
      421 gaccgtgacc tgactctggc tggacgcgtc attgtcaagt gccctacctc aggtcggctg
      481 gagcagcagc tggtgcacgt ggagcggccc attcttcgca tcccacacct ggccatccat
      541 ctgcagcgaa atatcaacga gaactttggg cccaacacag agatgcatct agtccccatt
      601 cttgccacag ccatccagga ggagctggag aaggggactc ctgagccagg gcctctcaat
      661 gctgtggatg agcggcacca ttcggtcctc atgtccctgc tctgtgccca tctggggctg
      721 agccccaagg acatagtgga gatggagctc tgccttgcag acacccagcc tgcggtcttg
      781 ggtggtgcct atgatgagtt catctttgct cctcggctgg acaatctgca cagctgcttc
      841 tgtgccctgc aggccttgat agattcctgt gcaggccctg gctccctggc cacagagcct
      901 cacgtgcgca tggtcacact ctatgacaac gaagaggtgg ggtctgagag tgcacaggga
      961 gcacagtcac tgctgacaga gctggtgctg cggcggatct cagcctcgtg ccagcacccg
     1021 acagccttcg aggaagccat acccaagtcc ttcatgatca gcgcagacat ggcccatgct
     1081 gtgcatccca actacctgga caagcatgag gagaaccacc ggcctttatt ccacaagggc
     1141 cccgtgatca aggtgaacag caagcaacgc tatgcttcaa acgcggtgtc agaggccctg
     1201 atccgagagg tggccaacaa agtcaaggtc cccctgcagg atctcatggt ccggaatgac
     1261 accccctgtg gaaccaccat tggacctatc ttggcttctc ggctggggct gcgggtgctg
     1321 gatttaggca gcccccaact ggccatgcac tctatccggg agatggcctg caccacagga
     1381 gtcctccaga ccctcaccct cttcaagggc ttctttgagc tgttcccttc tctagcagag
     1441 aacctctact tccaatcgca ccatcatcac caccatgatt acaaggatga cgacgataag
     1501 tgaggatcc
//
