LOCUS       sequence-490980-        4874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2639m is an enhancer sequence designed to drive AAV-mediated 
            transgene expression in specific population sof brain cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4874)
  TITLE     Basal Ganglia/CERP Enhancer AAVs
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2115 - pAAV-AiE2639m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..4874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      886..895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(892..894,895..897,898..1608)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(937..958)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1198..1217)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1545..1566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1701..1721)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1908..2019
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2137..2277
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2137..2266
                     /label=AAV2 ITR
     rep_origin      2352..2807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2439..2458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2649..2670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2824..2843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2943..2965
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3003..3021)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3089..3193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3194..3262,3263..4054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3412..3431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4225..4813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4714..4733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttccact atggtaacag tgccgtaaga gtcagacccc
      181 ctcacccctg actattaatg ttgtatggtg ctgcattgaa aaggaagatc tgacttactg
      241 tgagcttcat ggcggcagga gtgtggtgga gcagaggatc aggcggcgtg ctcacagaca
      301 ttcagccctc tgctatttgt acctgaccag ctttgctcaa gctaggtgtg ctcaggtgga
      361 gtccacggct tatctgcttc cctctcctgt atctgtggag ctgcagaggc ccggaagcct
      421 ttagctgcct gctcagtagg gcccttaggc aggaggcctc tgactcatcc tctgacgaag
      481 gtatctcagt aaactctaca cggtttgtga taagttctgg aatatggaag ccatgggtgt
      541 gggtatttta cgtattgctg ctgctcagat tcgggagaag agtaatccct aaaccaaaca
      601 tggccttaga tgagatctgt gtgtacacct gcccttcctg gtactggctc ctatgggaat
      661 gcgggaatgg tttcttctcc ttccctttac ccagtcctat tgtaagcttt ctccagctcc
      721 ttccaccctt atttccccct ctctatgctt cttgaccatg ctcaagtcat actgaaaact
      781 ttttcgccgg cggagctcgg gctgggcata aaagtcaggg cagagccatc tattgcttac
      841 atttgcttct gggatccaga tctttcgaag ctagcgctac cggtcgccac catggtgagc
      901 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      961 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1021 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     1081 accctgggct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     1141 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1201 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1261 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1321 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     1381 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     1441 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1501 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1561 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agtcgacggc
     1621 gcgccgcggc cgcgaattcg atatcataat caacctctgg attacaaaat ttgtgaaaga
     1681 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1741 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1801 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     1861 gctcggctgt tgggcactga caattccgtg gctcgagaga tcttcgactg tgccttctag
     1921 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     1981 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2041 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2101 caggcatgag atctcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     2161 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     2221 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     2281 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     2341 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2401 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     2461 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2521 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     2581 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2641 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     2701 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     2761 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     2821 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2881 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2941 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3001 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3061 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3421 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3721 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3781 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3841 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3901 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3961 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4021 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4081 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4141 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4201 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4261 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4321 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4381 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4441 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4501 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4561 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4621 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4681 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4741 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4801 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4861 ttttgctcac atgt
//
