LOCUS       sequence-49099-e        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MACROD1
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..357
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             364..384
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt atgagcgata aaattattca cctgactgac
       61 gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga tttctgggca
      121 gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc tgacgaatat
      181 cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
      241 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc
      301 aaagtgggcg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggccggt
      361 accgagaact tgtacttcca atccatgtcg gcgggagttg gggcgtgggg ggcggcggcg
      421 gtggggcgga cagccggggt gcgcacttgg gcccccctgg ccatggcggc gaaggtggac
      481 ctgagcacct ccaccgactg gaaggaggcg aaatcctttc tgaagggcct gagtgacaag
      541 cagcgggagg aacattactt ctgcaaggac tttgtcaggc tgaagaagat cccgacatgg
      601 aaggagatgg cgaaaggggt ggctgtgaag gtggaggagc ccaggtataa aaaggacaag
      661 cagctcaatg agaaaatctc cctgctccgc agcgacatca ccaagctgga ggtggacgcc
      721 atcgtcaacg ccgccaacag ctccctgctc ggaggcggtg gcgtggacgg ctgcattcat
      781 cgggccgccg gccccctgct taccgacgag tgccggaccc tgcagagctg taagactggc
      841 aaggccaaga tcaccggcgg ctatcggctc ccggccaagt acgtcatcca cacagtgggg
      901 cccatcgcct acggggagcc cagcgccagc caggctgccg agctccgcag ctgctacctg
      961 agcagtctgg acctgctgct ggagcaccgg ctccgctcgg tggcgttccc ctgcatctcc
     1021 accggcgtgt ttggctaccc ctgtgaggcg gccgccgaga tcgtgctggc cacgctgcga
     1081 gagtggctgg agcagcacaa ggacaaggtg gaccggctga tcatctgcgt gttcctcgag
     1141 aaggacgagg acatctaccg gagccggctc ccccactact tccccgtggc ctgacagtaa
     1201 aggtggatac ggatccgaat tcgagctccg tcgacaagct t
//
