LOCUS       sequence-491024-        6087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CytoTape signal monomer for recording SARE-ArcMin promoter 
            transcriptional activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: pAAV-Arc-CytoTape-V5
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             3002..3043
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3068..3275
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3445..3574
                     /label=AAV2 ITR
     rep_origin      complement(3811..4399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3891..3910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4570..5361,5362..5430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5193..5212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5431..5535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5561..6016)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5698..5719)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5910..5929
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg taggttaatt aaagcgcaca gagccttcct gcgtggggaa gctccttgct
      241 gcgtcatggc tcagctattc tcagcctctc tccttttatg gtgccggaag caggcaggct
      301 gctgctagat ctcgcgcagc agagcacatt agtcactcgg ggctgtgaag gggcgggtcc
      361 ttgagggcac ccacgggagg ggagcgagta ggcgcggaag gcggggcctg cggcaggaga
      421 gggcgcgggc gggctctggc gcggagcctg ggcgccgcca atgggagcca gggctccacg
      481 agctgccgcc cacgggcccc gcgcagcata aatagccgct ggtggcggtt tcggtgcaga
      541 gctcaagcga gttctcccgc agccgcagtc tctgggcctc tctagcttca gcggcgacga
      601 gcctgccaca ctcgctaagc tcctccggca ccgcacacct gccactgccg ctgcagccgc
      661 cggctctgct cccttccggc ttctgcctca gaggagttct tagcctgttc ggagccgcag
      721 caccgacgac cagactagtg ccaccatgat tgactatact gctgctggtt tcaccctcct
      781 ccaaggtgca catctgtacg ctcctgaaga cagaggtatc tgcgatgtgc tggttgccaa
      841 cggtaagatc atcgctgtgg cctccaacat tcctagcgac atcgttccaa actgcaccgt
      901 tgtcgatctg agcggacaga tcctctgtcc aggattcatt gatcagcacg tgcacctcat
      961 tggaggagga ggcgaagctg gtcctactac tagaactccc gaagtcgcac tgtctcgcct
     1021 cacagaggca ggcgttacct ccgtcgtggg actcctgggt actgatagca tctctaggca
     1081 ccctgagtct ctgctggcaa agaccagagc cctcaatgag gagggtatct ccgcttggat
     1141 gctgactggt gcttatcacg tcccatctcg caccattact ggcagcgtgg agaaggacgt
     1201 tgcaatcatc gacagagtga tcggtgttaa gtgcgctatc tccgaccaca ggagcgctgc
     1261 tcccgacgtc tatcacctgg ccaacatggc agccgagagc agagtcggtg gtctgctcgg
     1321 tggcaagcca ggagttaccg tgttccacat gggtgactct aagaaggcac tgcaacccat
     1381 ctatgacctg ctggagaact gtgacgtccc aatctccaag ctgctgccta cccacgtcaa
     1441 caggaacgtg cctctcttct accaagctct ggagttcgca agaaagggtg gcaccatcga
     1501 catcacctct agcattgatg aacccgtcgc tccagctgag ggaatcgcaa gggctgtcca
     1561 agcaggtatc ccactggcaa gggtgacact cagctctgat ggcaacggct cccagccatt
     1621 cttcgatgat gaaggaaatc tgactcacat tggcgtggct ggatttgaaa ctctcctgga
     1681 aacagttcag gttctggtta aggactacga cttcagcatt tctgacgctc tcagaccact
     1741 cacctctagc gtcgctggct tcctgaacct gactggtaaa ggcgaaatca aacctggtaa
     1801 tgacgctgat ctgctggtga tgacacccga actgcgcatt gagcaggtct atgcaagagg
     1861 aaagctgatg gttaaggacg gaaaggcttg tgtcaagggc acattcgaga ctgctggcaa
     1921 actggttatc tggatcaatg gcgataaggg ctacaatggc ctggcagagg tcggtaagaa
     1981 gttcgagaaa gatacaggca tcaaagttac tgtggaacac ccagataagc tggaggagaa
     2041 gttccctcag gtcgcagcaa ctggcgacgg tccagacatc atcttctggg cacacgatag
     2101 gttcggtggc tatgcccaat ctggcctgct ggctgagatt actcctgata aggctttcca
     2161 ggacaagctg tatccattca cctgggatgc tgtgagatac aatggcaagc tgatcgcata
     2221 ccctattgct gtggaagctc tgagcctgat ctacaacaag gacctgctgc ctaatcctcc
     2281 taagacatgg gaagaaatcc ctgcactgga caaggaactg aaggccaaag gcaagtccgc
     2341 actgatgttc aacctgcagg agccttactt tacctggcca ctgattgctg ccgacggtgg
     2401 ttacgctttc aagtacgaga atggtaagta cgacatcaaa gatgtgggtg tggacaatgc
     2461 tggtgccaaa gctggtctga ctttcctggt ggatctgatc aagaacaagc acatgaatgc
     2521 agacactgac tattctatcg cagaggctgc tttcaacaaa ggcgaaaccg caatgactat
     2581 caatggtcct tgggcatggt ctaacatcga cactagcaaa gtcaactacg gtgtcaccgt
     2641 tctgccaacc ttcaagggtc agccaagcaa acctttcgtt ggcgtgctga gcgcaggtat
     2701 caacgctgcc tctcctaaca aagagctggc taaggagttt ctggagaact acctgctgac
     2761 agacgaaggt ctggaggcag tgaacaagga caagccactg ggtgccgtgg ctctgaagag
     2821 ctacgaagaa gaactggcca aggaccctcg catcgctgct acaatggaga acgcacagaa
     2881 gggtgagatc atgccaaaca tcccacagat gagcgcattc tggtatgccg tgaggaccgc
     2941 tgttatcaac gcagcttctg gcagacagac cgtggatgaa gccctgaaag acgcacagac
     3001 cggtaagcct atccctaacc ctctgctcgg tctcgattct acctaagcgg ccgctcataa
     3061 agctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3121 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3181 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3241 gaggattggg aagacaatag caggcatgct ggggatgcgg tccggactgt acaattgcat
     3301 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     3361 ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc
     3421 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     3481 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3541 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc
     3601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     5581 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5641 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5701 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5761 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5821 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5881 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5941 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6001 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6061 gtgcgggcct cttcgctatt acgccag
//
