LOCUS       sequence-491025-        5895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CytoTape signal monomer for recording Npas4 protein activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: pAAV-N-RAM-CytoTape-V5
FEATURES             Location/Qualifiers
     source          1..5895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     CDS             2881..2922
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    2947..3154
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3324..3453
                     /label=AAV2 ITR
     rep_origin      complement(3690..4278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3770..3789)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4449..5240,5241..5309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5072..5091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5310..5414)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5440..5895)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5577..5598)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5789..5808
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaacgcgta ccctagaagt ttgttcgtga
      301 ctgtgactag aagtttgttc gtgactgtga ctagaagttt gttcgtgact gtgactagaa
      361 gtttgttcgt gactgtgaac tcattcataa aacgcttgtt ataaaagcag tggctgcggc
      421 gcctcgtact ccaaccgcat ctgcagcgag caactgagaa gccaagactg agccggcggc
      481 cggatccagc tcatgagcca tggcttcccg ccggaggtgg aggagcagga tgatggcacg
      541 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
      601 gctaggatca atgtggaatt caccatgatt gactatactg ctgctggttt caccctcctc
      661 caaggtgcac atctgtacgc tcctgaagac agaggtatct gcgatgtgct ggttgccaac
      721 ggtaagatca tcgctgtggc ctccaacatt cctagcgaca tcgttccaaa ctgcaccgtt
      781 gtcgatctga gcggacagat cctctgtcca ggattcattg atcagcacgt gcacctcatt
      841 ggaggaggag gcgaagctgg tcctactact agaactcccg aagtcgcact gtctcgcctc
      901 acagaggcag gcgttacctc cgtcgtggga ctcctgggta ctgatagcat ctctaggcac
      961 cctgagtctc tgctggcaaa gaccagagcc ctcaatgagg agggtatctc cgcttggatg
     1021 ctgactggtg cttatcacgt cccatctcgc accattactg gcagcgtgga gaaggacgtt
     1081 gcaatcatcg acagagtgat cggtgttaag tgcgctatct ccgaccacag gagcgctgct
     1141 cccgacgtct atcacctggc caacatggca gccgagagca gagtcggtgg tctgctcggt
     1201 ggcaagccag gagttaccgt gttccacatg ggtgactcta agaaggcact gcaacccatc
     1261 tatgacctgc tggagaactg tgacgtccca atctccaagc tgctgcctac ccacgtcaac
     1321 aggaacgtgc ctctcttcta ccaagctctg gagttcgcaa gaaagggtgg caccatcgac
     1381 atcacctcta gcattgatga acccgtcgct ccagctgagg gaatcgcaag ggctgtccaa
     1441 gcaggtatcc cactggcaag ggtgacactc agctctgatg gcaacggctc ccagccattc
     1501 ttcgatgatg aaggaaatct gactcacatt ggcgtggctg gatttgaaac tctcctggaa
     1561 acagttcagg ttctggttaa ggactacgac ttcagcattt ctgacgctct cagaccactc
     1621 acctctagcg tcgctggctt cctgaacctg actggtaaag gcgaaatcaa acctggtaat
     1681 gacgctgatc tgctggtgat gacacccgaa ctgcgcattg agcaggtcta tgcaagagga
     1741 aagctgatgg ttaaggacgg aaaggcttgt gtcaagggca cattcgagac tgctggcaaa
     1801 ctggttatct ggatcaatgg cgataagggc tacaatggcc tggcagaggt cggtaagaag
     1861 ttcgagaaag atacaggcat caaagttact gtggaacacc cagataagct ggaggagaag
     1921 ttccctcagg tcgcagcaac tggcgacggt ccagacatca tcttctgggc acacgatagg
     1981 ttcggtggct atgcccaatc tggcctgctg gctgagatta ctcctgataa ggctttccag
     2041 gacaagctgt atccattcac ctgggatgct gtgagataca atggcaagct gatcgcatac
     2101 cctattgctg tggaagctct gagcctgatc tacaacaagg acctgctgcc taatcctcct
     2161 aagacatggg aagaaatccc tgcactggac aaggaactga aggccaaagg caagtccgca
     2221 ctgatgttca acctgcagga gccttacttt acctggccac tgattgctgc cgacggtggt
     2281 tacgctttca agtacgagaa tggtaagtac gacatcaaag atgtgggtgt ggacaatgct
     2341 ggtgccaaag ctggtctgac tttcctggtg gatctgatca agaacaagca catgaatgca
     2401 gacactgact attctatcgc agaggctgct ttcaacaaag gcgaaaccgc aatgactatc
     2461 aatggtcctt gggcatggtc taacatcgac actagcaaag tcaactacgg tgtcaccgtt
     2521 ctgccaacct tcaagggtca gccaagcaaa cctttcgttg gcgtgctgag cgcaggtatc
     2581 aacgctgcct ctcctaacaa agagctggct aaggagtttc tggagaacta cctgctgaca
     2641 gacgaaggtc tggaggcagt gaacaaggac aagccactgg gtgccgtggc tctgaagagc
     2701 tacgaagaag aactggccaa ggaccctcgc atcgctgcta caatggagaa cgcacagaag
     2761 ggtgagatca tgccaaacat cccacagatg agcgcattct ggtatgccgt gaggaccgct
     2821 gttatcaacg cagcttctgg cagacagacc gtggatgaag ccctgaaaga cgcacagacc
     2881 ggtaagccta tccctaaccc tctgctcggt ctcgattcta cctaagcggc cgctcataaa
     2941 gctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3001 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3061 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3121 aggattggga agacaatagc aggcatgctg gggatgcggt ccggactgta caattgcatt
     3181 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     3241 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca
     3301 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3361 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3421 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcatt aatgaatcgg ccaacgcgcg
     3481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5401 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     5461 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     5521 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     5581 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     5641 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     5701 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5761 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     5821 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     5881 cgctacaggg cgcgt
//
