LOCUS       sequence-491027-        6252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CytoTape signal monomer for recording phosphorylated CREB protein 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Zheng L, Yan Y, Zhou B, Lim J, Shi D, An B, Ko B, Klyder E, 
            Pitchiaya S, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Multiplexed, scalable analog recording of gene regulation dynamics 
            over weeks using intracellular protein tapes
  JOURNAL   bioRxiv 2025.05.10.653182
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.10.653182"
COMMENT     Sequence Label: pAAV-CRE-CytoTape-V5
FEATURES             Location/Qualifiers
     source          1..6252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     protein_bind    280..304
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(567..587)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        603..640
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     637..661
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    723..743
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        770..788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(852..870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(894..918)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3238..3279
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3304..3511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3681..3810
                     /label=AAV2 ITR
     rep_origin      complement(4047..4635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4127..4146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4806..5597,5598..5666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5429..5448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5667..5771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5797..6252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5934..5955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6146..6165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaacgcgta caagtttgta caaaaaagca
      301 ggctccctaa ctggccggta ccgagctctt acgcgtgcta gcgcaccaga cagtgacgtc
      361 agctgccaga tcccatggcc gtcatactgt gacgtctttc agacacccca ttgacgtcaa
      421 tgggagaaca gatctgccgc cccgactagc gcaccagaca gtgacgtcag ctgccagatc
      481 ccatggccgt catactgtga cgtctttcag acaccccatt gacgtcaatg ggagaacaga
      541 tctgccgccc cgactagcgg ggggctcaac ttttctatac aaagttgcgg tacccgggtc
      601 gagtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
      661 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      721 cccaactttg tataataaag ttgagctagt tgctaagtct gccgagtaga attaaccctc
      781 actaaagggt aggtgacact atcaaaacat tactacattc taacacggtg ttctacaaac
      841 ttttactctt tcctatagtg agtcgtatta gtagacgcga gtcaatgcat gtacgacagc
      901 tttcttgtac aaagtggttc gatatcaaga tctggcctcg gcggccaagc ttggcaatcc
      961 ggtactgttg gtaaagccac catgattgac tatactgctg ctggtttcac cctcctccaa
     1021 ggtgcacatc tgtacgctcc tgaagacaga ggtatctgcg atgtgctggt tgccaacggt
     1081 aagatcatcg ctgtggcctc caacattcct agcgacatcg ttccaaactg caccgttgtc
     1141 gatctgagcg gacagatcct ctgtccagga ttcattgatc agcacgtgca cctcattgga
     1201 ggaggaggcg aagctggtcc tactactaga actcccgaag tcgcactgtc tcgcctcaca
     1261 gaggcaggcg ttacctccgt cgtgggactc ctgggtactg atagcatctc taggcaccct
     1321 gagtctctgc tggcaaagac cagagccctc aatgaggagg gtatctccgc ttggatgctg
     1381 actggtgctt atcacgtccc atctcgcacc attactggca gcgtggagaa ggacgttgca
     1441 atcatcgaca gagtgatcgg tgttaagtgc gctatctccg accacaggag cgctgctccc
     1501 gacgtctatc acctggccaa catggcagcc gagagcagag tcggtggtct gctcggtggc
     1561 aagccaggag ttaccgtgtt ccacatgggt gactctaaga aggcactgca acccatctat
     1621 gacctgctgg agaactgtga cgtcccaatc tccaagctgc tgcctaccca cgtcaacagg
     1681 aacgtgcctc tcttctacca agctctggag ttcgcaagaa agggtggcac catcgacatc
     1741 acctctagca ttgatgaacc cgtcgctcca gctgagggaa tcgcaagggc tgtccaagca
     1801 ggtatcccac tggcaagggt gacactcagc tctgatggca acggctccca gccattcttc
     1861 gatgatgaag gaaatctgac tcacattggc gtggctggat ttgaaactct cctggaaaca
     1921 gttcaggttc tggttaagga ctacgacttc agcatttctg acgctctcag accactcacc
     1981 tctagcgtcg ctggcttcct gaacctgact ggtaaaggcg aaatcaaacc tggtaatgac
     2041 gctgatctgc tggtgatgac acccgaactg cgcattgagc aggtctatgc aagaggaaag
     2101 ctgatggtta aggacggaaa ggcttgtgtc aagggcacat tcgagactgc tggcaaactg
     2161 gttatctgga tcaatggcga taagggctac aatggcctgg cagaggtcgg taagaagttc
     2221 gagaaagata caggcatcaa agttactgtg gaacacccag ataagctgga ggagaagttc
     2281 cctcaggtcg cagcaactgg cgacggtcca gacatcatct tctgggcaca cgataggttc
     2341 ggtggctatg cccaatctgg cctgctggct gagattactc ctgataaggc tttccaggac
     2401 aagctgtatc cattcacctg ggatgctgtg agatacaatg gcaagctgat cgcataccct
     2461 attgctgtgg aagctctgag cctgatctac aacaaggacc tgctgcctaa tcctcctaag
     2521 acatgggaag aaatccctgc actggacaag gaactgaagg ccaaaggcaa gtccgcactg
     2581 atgttcaacc tgcaggagcc ttactttacc tggccactga ttgctgccga cggtggttac
     2641 gctttcaagt acgagaatgg taagtacgac atcaaagatg tgggtgtgga caatgctggt
     2701 gccaaagctg gtctgacttt cctggtggat ctgatcaaga acaagcacat gaatgcagac
     2761 actgactatt ctatcgcaga ggctgctttc aacaaaggcg aaaccgcaat gactatcaat
     2821 ggtccttggg catggtctaa catcgacact agcaaagtca actacggtgt caccgttctg
     2881 ccaaccttca agggtcagcc aagcaaacct ttcgttggcg tgctgagcgc aggtatcaac
     2941 gctgcctctc ctaacaaaga gctggctaag gagtttctgg agaactacct gctgacagac
     3001 gaaggtctgg aggcagtgaa caaggacaag ccactgggtg ccgtggctct gaagagctac
     3061 gaagaagaac tggccaagga ccctcgcatc gctgctacaa tggagaacgc acagaagggt
     3121 gagatcatgc caaacatccc acagatgagc gcattctggt atgccgtgag gaccgctgtt
     3181 atcaacgcag cttctggcag acagaccgtg gatgaagccc tgaaagacgc acagaccggt
     3241 aagcctatcc ctaaccctct gctcggtctc gattctacct aagcggccgc tcataaagct
     3301 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3361 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3421 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3481 attgggaaga caatagcagg catgctgggg atgcggtccg gactgtacaa ttgcattcat
     3541 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     3601 aaatgtggta aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg
     3661 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     3721 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3781 ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg
     3841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5761 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     5821 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5881 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5941 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6001 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6061 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6121 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6181 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6241 tacagggcgc gt
//
