LOCUS       sequence-491051-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of fluorescent membrane scaffold protein MSP1D1
            fused to sfGFP for nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  TITLE     Fluorescent Nanodisc Scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSP1D1_sfGFP
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggtcatc atcatcatca tcatcacgat tatgatattc ctactactga gaatttgtat
       61 tttcagggtt ctaccttcag taaacttcgc gaacaactgg gccccgtgac gcaggaattc
      121 ttcgacaacc tggaaaaaga aaccgaggga ctgcgtcagg aaatgtccaa agatctagaa
      181 gaggtgaagg ccaaggttca gccatatctc gatgactttc agaaaaaatt ccaggaagag
      241 atggaattat atcgtcaaaa ggtggaaccg ctgcgtgcgg aactgcaaga gggagcaaga
      301 caaaaactcc atgagctcca agagaagctc agcccattag gcgaagaaat gcgcgatcgc
      361 gcccgtgcac atgttgatgc actccggact catttggcgc cgtattcgga tgaacttcgc
      421 cagcgtttgg ccgcacgtct cgaggcgctg aaagaaaacg ggggtgcccg cttggctgag
      481 taccacgcga aagcgacaga acacctgagc accttgagcg aaaaagcgaa accggcgctg
      541 gaagatctac gccagggctt attgcctgtt cttgagagct ttaaagtcag ttttctgtca
      601 gctctggaag aatatactaa aaagctgaat acccag
//
