LOCUS       sequence-491052-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of fluorescent membrane scaffold protein MSP1D1
            fused to sfGFP for nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  TITLE     Fluorescent Nanodisc Scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSP1D1_sfGFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tctaaaggtg aggaactgtt taccggcgtt gttccgattc tggtcgagct ggatggtgac
       61 gtaaacggcc acaaattctc cgtgcgtggt gagggcgagg gcgacgcgac caatggcaag
      121 ctgaccctca agttcatctg caccacgggt aagctgccgg ttccgtggcc tacgctggtg
      181 accacgttga cctatggtgt tcaatgtttt tctcgttacc cggaccacat gaaacgccat
      241 gattttttta aaagcgcaat gccggaaggc tatgttcagg agagaactat tagctttaag
      301 gacgacggca cctacaagac ccgtgcggaa gtcaaatttg aaggtgacac cctggtgaat
      361 cgtattgaat tgaagggtat cgacttcaaa gaagatggaa acattctggg tcacaagttg
      421 gagtacaatt tcaactctca taacgtctat atcacggctg ataaacagaa aaacggtatc
      481 aaagccaact tcaagatccg ccacaacgtg gaggacggca gcgtgcaact ggcggatcac
      541 taccagcaaa acaccccgat tggtgacggc ccagttttgc ttccggataa tcattacctg
      601 tccacccaga gcgtgctgag caaggacccg aatgaaaaac gtgatcacat ggttttgctg
      661 gagttcgtga ctgcggcagg gatcacccac ggtatggatg aattatataa g
//
