LOCUS       sequence-491063-         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of fluorescent membrane scaffold protein MSP1D1
            fused to sfCherry for nanodisc formation (longer linker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  TITLE     Fluorescent Nanodisc Scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSP1D1_sfCherryL
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggacatc accaccacca ccaccatgat tacgacatcc cgaccaccga aaatctgtac
       61 ttccaaggct cgacctttag caaactgcgt gaacagttgg gtccggttac ccaagagttc
      121 tgggataacc tggagaaaga aaccgagggc ctgcgccaag aaatgagcaa ggacctcgag
      181 gaagtgaagg cgaaggtcca gccgtatctg gatgattttc agaaaaagtg gcaggaggaa
      241 atggagctgt accgccaaaa agtagaaccg ttgcgtgcgg agttgcaaga gggcgcgcgt
      301 cagaagctgc acgagctgca agagaaactg tccccgctgg gtgaagagat gcgtgatcgt
      361 gcgagagcac atgttgatgc gttgcgcacc cacctggcac cgtacagcga tgaactacgc
      421 caacgtctgg cggcacgtct tgaggcgctg aaagagaacg gcggcgctcg tttggctgag
      481 tatcatgcaa aggctaccga acacttatct acgctgtctg agaaggcgaa accggctttg
      541 gaggacttgc gccaaggcct gctgccggtg ctggagagct tcaaagtgag cttcctgagc
      601 gcattggagg agtacaccaa aaaattaaat acccag
//
