LOCUS       sequence-491070-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Halo-tagged membrane scaffold protein MSP1D1
            fused to HaloTag for nanodisc formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  TITLE     Fluorescent Nanodisc Scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMSP1D1_HaloTag
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             901..918
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(971..989)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      985..1032
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcgatcggga caggattccc atttgatccc cactatgttg aagttctggg cgagcgcatg
       61 cattacgtgg acgttggtcc aagagatggc accccggttc tgttccttca tggtaacccg
      121 acgagctcct acgtgtggcg taatatcatt ccgcacgtcg cgccaaccca tcgttgtatc
      181 gcaccggacc tgatcggcat gggcaagtct gataaaccgg atttgggcta tttctttgat
      241 gaccacgtgc gcttcatgga tgcgtttatt gaagcgttgg gcctggagga ggtggtgctg
      301 gttatccatg actggggttc tgccctgggt tttcattggg caaagcgtaa cccggagcgt
      361 gttaaaggta ttgcgttcat ggagttcatc cgtccgattc cgacttggga tgaatggccg
      421 gagttcgcgc gtgaaacctt tcaggcattc cgcaccaccg acgtcggtcg taaactgatt
      481 atcgatcaca acgtgtttat cgaaggtacg ctgcgcatgg gtgtggtacg tccgttgacc
      541 gaagttgaga tggaccacta ccgtgagccg tttctgaatc cggttgaccg tgagccgctg
      601 tggcgtttcc cgaatgagct gcctatcgcg ggcgaaccgg ctaatattgt cgccttggtg
      661 gaagagtata tggactggct gcaccagtcg ccggtgccga agttgctgtt ttggggcacc
      721 ccgggcgttc tgattcctcc ggcggaggcg gcacgcctgg ctaagagctt gccgaactgc
      781 aaagctgtgg atattggtcc gggtctgaac ctgcttcaag aagacaaccc cgacctcatc
      841 ggctccgaaa tcgcccgctg gttaagcacc ctggagatta gctaagcggc cgcactcgag
      901 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      961 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1021 aggggttttt tgctgaaagg aggaactata tccggat
//
