LOCUS       sequence-49109-e         478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 478)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ASH1L
FEATURES             Location/Qualifiers
     source          1..478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg aagtggcgcg tgcggcgcgt ctggcccaga tttttaaaga aatttgcgat
      121 ggcattatta gctataaaga tagcagccgt caggcgctgg ccgccccgct gctgaacctg
      181 ccgccgaaaa aaaaaaatgc cgattattat gaaaaaatta gcgatccgct ggatctgatt
      241 accatcgaaa aacagattct gaccggttat tataaaaccg tggaagcgtt tgatgccgat
      301 atgctgaaag tgtttcgtaa tgccgaaaaa tattatggtc gcaaaagccc ggttggccgc
      361 gatgtttgtc gcctgcgtaa agcgtattat aacgcccgtc atgaagcgag cgcccagatt
      421 gatgaaattg tgggcgaaac cgccagcgaa tgacagtaaa ggtggatacg gatccgaa
//
