LOCUS       sequence-49110-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: H2AFY2
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..357
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             364..384
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt atgagcgata aaattattca cctgactgac
       61 gacagttttg acacggatgt actcaaagcg gacggggcga tcctcgtcga tttctgggca
      121 gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc tgacgaatat
      181 cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
      241 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc
      301 aaagtgggcg cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggccggt
      361 accgagaact tgtacttcca atccatgaaa gaaggaactt caaattccac ctctgaagat
      421 gggccagggg atggattcac cattctgtct tctaagagcc ttgttctggg acagaagctg
      481 tccttaaccc agagtgacat cagccatatt ggctccatga gagtggaggg cattgtccac
      541 ccaaccacag ccgaaattga cctcaaagaa gatataggta aagccttgga aaaggctggg
      601 ggaaaagagt tcttggaaac ggtaaaggag cttcgcaaat cccaaggccc tttggaagtc
      661 gccgaagccg ccgtcagcca atccagtgga ctcgcagcca aatttgtcat ccactgtcac
      721 atccctcagt ggggctccga caaatgtgaa gaacagcttg aagagaccat caaaaactgc
      781 ctgtcagcgg cggaggacaa gaagctaaag tccgtcgcgt tcccgccttt ccccagcggc
      841 agaaactgct ttcccaaaca gactgcggcc caggtgaccc tcaaagccat ctcagcccac
      901 tttgatgact cgagcgcgtc ctcgctgaag aacgtgtact tcctgctctt cgacagcgag
      961 agcatcggca tctacgtgca ggagatggcc aagctcgacg ccaagtgaca gtaaaggtgg
     1021 atacggatcc gaattcgagc tccgtcgaca agctt
//
